In vitro propagation of Gerbera jamesonii Bolus ex Hooker f. in a temporary immersion bioreactor

Psychotria ipecacuanha, is a plant species with known medicinal properties that is critically endangered due to overexploitation of natural populations. Although the difficulties in conventional propagation by seed and by vegetative propagation are generally understood, the present study enhances our knowledge by describing efficient plant regeneration and root induction protocols for P. ipecacuanha while comparing alkaloid content (emetine and cephaeline) in in vitro-derived tissues. Stem node explants were cultured on MS medium MS supplemented with indolbutiric acid (IBA) in semi-solid media and the RITA® temporary immersion bioreactor. The highest root formation (81%) was in MS + 1.5 mg L−1 IBA in the bioreactor. After 24 months of acclimatization, the plants cultivated in MS + 0.50 and 1.0 mg L-1 of IBA had the highest number of roots (3), with mean values of 10.47 and 9.40 cm, respectively. The cultures coming from 1.0 mg L−1 and 0.5 mg L−1 IBA in the bioreactor contained higher cephaeline content, with a relative area of 14.2 and 14.9%, respectively. For emetine, the 1.0 mg L−1 IBA cultures in the bioreactor, 0.5 mg L−1 IBA and MS0 cultures contained higher content than the other treatments, with a relative area of 10.2, 10.2 and 10.1%, respectively.

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Improvement of In Vitro Proliferation and Elongation of Habanero Pepper Shoots (Capsicum chinense Jacq.) by Temporary Immersion

HortScience ◽

10.21273/hortsci.45.7.1093 ◽

2010 ◽

Vol 45 (7) ◽

pp. 1093-1098 ◽

Cited By ~ 9

Author(s):

Jericó J. Bello-Bello ◽

Adriana Canto-Flick ◽

Eduardo Balam-Uc ◽

Eunice Gómez-Uc ◽

Manuel L. Robert ◽

...

Keyword(s):

Shoot Elongation ◽

Nodal Segments ◽

High Survival ◽

Shoot Induction ◽

Capsicum Chinense ◽

Temporary Immersion Bioreactor ◽

In Vitro Proliferation ◽

Temporary Immersion ◽

Habanero Pepper

This article describes the performance of nodal segments from Habanero pepper (Capsicum. chinense) during shoot induction and elongation under different semisolid and liquid culture conditions with various degrees of ventilation in which they were exposed to different levels of immersion and growth regulators. The ethylene content in non-ventilated containers, the age of the explant donor plants as well as the effect of thidiazuron and paclobutrazol on shoot induction and of gibberellic acid and AgNO3 on shoot elongation were also evaluated. A temporary immersion bioreactor (BioMINT™) was used for the multiplication and elongation of isolated shoots with very good results. We report an efficient protocol for the in vitro propagation of Habanero pepper that produces plants with a high survival rate when transplanted to soil.

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Use of temporary immersion bioreactors and solid culture medium in the in vitro propagation of pear rootstocks

Acta Horticulturae ◽

10.17660/actahortic.2021.1303.17 ◽

2021 ◽

pp. 113-120

Author(s):

A.L. Arruda ◽

F.R. Nerbass ◽

A.A. Kretzschmar ◽

L. Rufato ◽

A.J. Posser ◽

...

Keyword(s):

In Vitro Propagation ◽

Culture Medium ◽

Solid Culture ◽

Temporary Immersion ◽

Solid Culture Medium

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In vitro-grown roots: a superior explant for prolific shoot regeneration of St. John's wort (Hypericum perforatum L. cv ‘New Stem’) in a temporary immersion bioreactor

Plant Science ◽

10.1016/s0168-9452(03)00064-5 ◽

2003 ◽

Vol 165 (3) ◽

pp. 463-470 ◽

Cited By ~ 47

Author(s):

S.M.A. Zobayed ◽

P.K. Saxena

Keyword(s):

Shoot Regeneration ◽

Hypericum Perforatum ◽

Temporary Immersion Bioreactor ◽

Temporary Immersion ◽

St John’S Wort ◽

St John's Wort ◽

Hypericum Perforatum L ◽

Prolific Shoot

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Biorreator na micropropagação de abacaxi ornamental

Ornamental Horticulture ◽

10.14295/oh.v24i2.1181 ◽

2018 ◽

Vol 24 (2) ◽

pp. 182-187

Author(s):

Caroline Oliveira Reis ◽

Adriano Bortolotti Silva ◽

Paulo Roberto Landgraf ◽

Jessica Azevedo Batisita ◽

Guilherme Antonio Jacome

Keyword(s):

Natural Ventilation ◽

Ananas Comosus ◽

Basic Medium ◽

In Vitro Cultivation ◽

Temporary Immersion Bioreactor ◽

Temporary Immersion ◽

Time Period ◽

Culture Growth ◽

Short Time

Ornamental pineapple is a hard plant with significant landscaping value. Typically, conventional propagation is performed by clump division with low yields, and may even spread diseases. Plant tissue culture is viable, yielding plants with a high phytosanitary and genetic quality over a short time period. This study aimed to verify the in vitro multiplication of ornamental pineapple plants (Ananas comosus var. bracteatus L.) in different micropropagation systems, in association with BAP concentrations. Plants with about 2 cm were used, transplanted to the different treatments: bioreactor, natural ventilation and conventional micropropagation, combined with 3 BAP concentrations (0, 1 and 2 mg L-1). The basic medium used consisted of MS salts. The highest number of shoots and in vitro culture growth were obtained with the use of bioreactor and culture medium containing 2 mg L-1 BAP. The temporary immersion bioreactor allows air renewal inside the bottles, leading to a better performance of in vitro cultivation of ornamental pineapple, when compared to conventional micropropagation.

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Propagasi in vitro tanaman kurma (Phoenix dactylifera L.) pada bioreaktor dengan perendaman sesaat

E-Journal Menara Perkebunan ◽

10.22302/iribb.jur.mp.v88i2.394 ◽

2020 ◽

Vol 88 (2) ◽

Author(s):

Rizka Tamania SAPTARI ◽

Masna Maya SINTA ◽

Imron RIYADI ◽

. PRIYONO ◽

. SUMARYONO

Keyword(s):

Somatic Embryogenesis ◽

Somatic Embryos ◽

In Vitro Propagation ◽

Date Palm ◽

Phoenix Dactylifera ◽

Embryogenic Calli ◽

Temporary Immersion Bioreactor ◽

Phoenix Dactylifera L ◽

Immersion Period

The cultivation of date palm in Indonesia has increased since the last decade. However, the superior date palm seedlings are still limited and most of them are imported from other countries. The mass supply of superior date palm seedlings can be provided by in vitro propagation in the bioreactor. Therefore, the research was conducted to develop a protocol of date palm in vitro propagation by using Temporary Immersion Bioreactor (TIB). The in vitro propagation was carried out through somatic embryogenesis technique using meristematic tissues isolated from offshoots of date palm female clone cv. Zambli as explants. The explants were sterilized and then cultured to produce embryogenic calli and somatic embryos. Afterwards, somatic embryos germination and plantlets formation were conducted in TIB with treatments of immersion period: 3, 10, and 30 minutes every 6 hours, with 8 replications, The results showed that the optimal somatic embryo germination in TIB was with the immersion period of 30 min every 6 h, resulting in the most formation of shoots and fresh biomass weight increment up to nearly threefold in 6 weeks. Thereafter, plantlets formation in TIB with immersion period of 10 min and 30 min every 6 h exhibited similar performances in producing more plantlets with higher total fresh weight and better vigor than those of 3 min every 6 h. However, there were more rooted plantlets in the TIB with immersion period of 10 min every 6 h. Based on the results, an in vitro propagation protocol via somatic embryogenesis in TIB has been successfully developed for mass propagation of date palm cv. Zambli, which produced plantlets with good vigor and rooting.

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