scholarly journals Elicitation of Isatis tinctoria L. hairy root cultures by salicylic acid and methyl jasmonate for the enhanced production of pharmacologically active alkaloids and flavonoids

2019 ◽  
Vol 137 (1) ◽  
pp. 77-86 ◽  
Author(s):  
Qing-Yan Gai ◽  
Jiao Jiao ◽  
Xin Wang ◽  
Yu-Ping Zang ◽  
Li-Li Niu ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Methyl Jasmonate ◽  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Isatis Tinctoria ◽  
Enhanced Production ◽  
Pharmacologically Active

scholarly journals Enhanced production of valtrate in hairy root cultures of Valeriana jatamansi Jones by methyl jasmonate, jasmonic acid and salicylic acid elicitors

2020 ◽  
Vol 48 (2) ◽  
pp. 839-848
Author(s):  
Shuang ZHAO ◽  
Hong TANG
Keyword(s):  
Salicylic Acid ◽  
Jasmonic Acid ◽  
Methyl Jasmonate ◽  
Hairy Root ◽  
Hairy Roots ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Potent Inducer ◽  
Valeriana Jatamansi ◽  
Pharmacologically Active

Valtrate is a pharmacologically active epoxyiridoid ester found in the roots and rhizomes of Valeriana jatamansi Jones. The plant produces only small amounts of this metabolite naturally, and so induction of hairy roots as well as elicitation can be useful to increase its commercial production. In this study, strain R1601 of Agrobacterium rhizogenes was used to induce hairy roots in V. jatamansi, and stable hairy root cultures of V. jatamansi were established successfully. The influence of three exogenous elicitors including methyl jasmonate (MJ), jasmonic acid (JA) and salicylic acid (SA) on valtrate production in the hairy root cultures of V. jatamansi was also investigated, and the 25-day-old hairy root cultures were treated with different concentrations of the elicitors at exposure time of 7 days. This present study showed that MJ (100 mg/L) highly promoted valtrate production at 7 days after elicitation, to a level of 3.63 times higher than that of non-elicited control. SA did not significantly increase the production of valtrate. This is the first-time study to assess the elicitation of hairy root cultures to promote valtrate biosynthesis in V. jatamansi and the resulting experiments demonstrated that MJ was indeed a potent inducer of valtrate biosynthesis.

Enhanced Production of Resveratrol, Piceatannol, Arachidin-1, and Arachidin-3 in Hairy Root Cultures of Peanut Co-treated with Methyl Jasmonate and Cyclodextrin

2015 ◽  
Vol 63 (15) ◽  
pp. 3942-3950 ◽  
Author(s):  
Tianhong Yang ◽  
Lingling Fang ◽  
Cesar Nopo-Olazabal ◽  
Jose Condori ◽  
Luis Nopo-Olazabal ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Enhanced Production

Enhanced production of asiaticoside from hairy root cultures of Centella asiatica (L.) Urban elicited by methyl jasmonate

2007 ◽  
Vol 26 (11) ◽  
pp. 1941-1949 ◽  
Author(s):  
Ok-Tae Kim ◽  
Kyong-Hwan Bang ◽  
Yu-Su Shin ◽  
Min-Jeong Lee ◽  
Su-Jin Jung ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Hairy Root ◽  
Centella Asiatica ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Enhanced Production

Increased production of withanolide A, withanone, and withaferin A in hairy root cultures of Withania somnifera (L.) Dunal elicited with methyl jasmonate and salicylic acid

2013 ◽  
Vol 114 (1) ◽  
pp. 121-129 ◽  
Author(s):  
Ganeshan Sivanandhan ◽  
Gnanajothi Kapil Dev ◽  
Murugaraj Jeyaraj ◽  
Manoharan Rajesh ◽  
Annavi Arjunan ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Methyl Jasmonate ◽  
Hairy Root ◽  
Withania Somnifera ◽  
Withaferin A ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Withanolide A

Enhanced production of falcarinol-type polyacetylenes in hairy roots of cultivated carrot (Daucus carota subsp. sativus)

2021 ◽  
Author(s):  
Frank Dunemann ◽  
Christoph Böttcher
Keyword(s):  
Hairy Root ◽  
Large Scale ◽  
Higher Plants ◽  
Leaf Tissue ◽  
Hairy Root Cultures ◽  
Scale Production ◽  
Root Cultures ◽  
Tissue Samples ◽  
Positive Effects ◽  
Enhanced Production

Abstract Polyacetylenes (PAs) are a large group of bioactive phytochemicals, which are primarily produced by higher plants of the families Apiaceae and Araliaceae. Especially aliphatic C17-polyacetylenes of the falcarinol-type such as falcarinol (FaOH) and falcarindiol (FaDOH) are known for their numerous positive effects on human health. In this study we investigate the potential of carrot hairy root cultures for production of PAs. Three individual plants of seven differently coloured carrot cultivars were used for the development of hairy root cultures by transformation of root discs with the wild-type Rhizobium rhizogenes strain 15834. A total of 51 individual hairy root (HR) lines were obtained and quantitatively analysed together with root, petiole and leaf tissue samples for FaOH and FaDOH. Among the five tissues sampled from the donor plants, root periderm samples generally exhibited the highest PA levels with FaDOH as prevailing PA and large differences between cultivars. In comparison to periderm tissue, FaOH levels were highly increased in HR lines of all cultivars. In contrast, FaDOH levels were not significantly altered. Considering the low to moderate PA concentration in root and leaf tissues of the orange cultivars there was an up to more than 10-fold increase of the FaOH concentration in HRs of these genotypes. Within this study a reproducible method for Rrhi-mediated transformation of carrot root discs was applied which provides an efficient tool to assess the function of candidate genes involved in the biosynthesis of key PAs in carrot but might be used in future also for the large-scale production of falcarinol-type PAs.

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