Histological and cytological study on meristematic nodule induction and shoot organogenesis in Paeonia ostii ‘Feng Dan’

Cytological examination of smears from the sediment after centrifugation of pleural fluids was performed in 479 patients who underwent examination and treatment at our institution in 2014-2016. In 249 (52%) patients tumor cells were not detected in smears, in 230 (48%) observations a suspicion (28 observations) or a confident conclusion (202 observations) on the presence of malignant tumor cells in the exudates was cytologically expressed. In 38 cases immunocytochemical studies was additionally performed. In two observations a false-negative conclusion about the absence of tumor cells in smears was expressed. The sensitivity of the cytological study in the diagnosis of malignant pleuritis was 99.0%. Affirmative cytological conclusions on the presence of malignant pleuritis were given in 87.0% of observations, suspicious cytological responses - in 12.0% of cases. Immunocytochemical studies significantly expanded the possibilities of cytological research and were of great importance in the diagnosis of metastases of tumors of unknown primary localization.

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Influences of Growth Regulators and Cultivars on Callus and Shoot Production of Alfalfa

HortScience ◽

10.21273/hortsci.33.3.461f ◽

1998 ◽

Vol 33 (3) ◽

pp. 461f-462

Author(s):

Guochen Yang ◽

Marihelen Kamp-Glass

Keyword(s):

Growth Regulators ◽

Culture Medium ◽

Shoot Organogenesis ◽

Multiple Shoots ◽

Apparent Difference ◽

Shoot Production ◽

Callus Initiation ◽

Dark Green ◽

Number Of Shoots ◽

Alfalfa Seeds

Alfalfa seeds of Cimarron VR, CW1446, CW2440, C94-118, C94-785, and WL311 were used as explants. BA, zeatin, and TDZ were evaluated on callus initiation, development, and shoot production. Callus initiation and development toward shoot organogenesis were enhanced when BA was added in the culture medium. Calli produced from BA treatments were compact, solid, and dark green. Similar results were obtained when zeatin was added in the culture medium. However, no shoots were produced from such calli. Multiple shoots were produced directly from each individual explant when TDZ was added to culture medium. However, when higher concentration of TDZ was used, number of shoots per explant was decreased, and dwarf shoots were produced. No apparent difference on shoot production was observed among the cultivars tested so far. Data on number of shoots per explant from two of these cultivars need to be statistically analyzed.

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Fragile X Syndrome: Clinical & Cytological Study in North Karnataka

International Journal of Pharma and Bio Sciences ◽

10.22376/ijpbs.2017.8.2.b27-30 ◽

2017 ◽

Vol 8 (2) ◽

Author(s):

DR G.S.KADA KOL ◽

DR M M PA TIL ◽

DR S V PATIL ◽

DR.BHEEMSHETTY S. PATIL ◽

DR R S BULA GOUDA

Keyword(s):

Fragile X Syndrome ◽

Cytological Study ◽

Fragile X

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Cytological Study on Induced Apospory in Ferns

CYTOLOGIA ◽

10.1508/cytologia.27.79 ◽

1962 ◽

Vol 27 (1) ◽

pp. 79-96 ◽

Cited By ~ 9

Author(s):

Chihiro Takahashi

Keyword(s):

Cytological Study

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Thidiazuron-induced shoot organogenesis and production of hepatoprotective lignan phyllanthin and hypophyllanthin in Phyllanthus amarus

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-010-9796-3 ◽

2010 ◽

Vol 104 (1) ◽

pp. 101-110 ◽

Cited By ~ 19

Author(s):

K. M. Nitnaware ◽

D. G. Naik ◽

T. D. Nikam

Keyword(s):

Shoot Organogenesis ◽

Phyllanthus Amarus

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Natural Variation in Plant Pluripotency and Regeneration

Plants ◽

10.3390/plants9101261 ◽

2020 ◽

Vol 9 (10) ◽

pp. 1261

Author(s):

Robin Lardon ◽

Danny Geelen

Keyword(s):

De Novo ◽

Shoot Organogenesis ◽

In Vitro Regeneration ◽

Relative Role ◽

Root Explants ◽

Experimental Systems ◽

Complex Process ◽

Insight Into ◽

Molecular Framework

Plant regeneration is essential for survival upon wounding and is, hence, considered to be a strong natural selective trait. The capacity of plant tissues to regenerate in vitro, however, varies substantially between and within species and depends on the applied incubation conditions. Insight into the genetic factors underlying this variation may help to improve numerous biotechnological applications that exploit in vitro regeneration. Here, we review the state of the art on the molecular framework of de novo shoot organogenesis from root explants in Arabidopsis, which is a complex process controlled by multiple quantitative trait loci of various effect sizes. Two types of factors are distinguished that contribute to natural regenerative variation: master regulators that are conserved in all experimental systems (e.g., WUSCHEL and related homeobox genes) and conditional regulators whose relative role depends on the explant and the incubation settings. We further elaborate on epigenetic variation and protocol variables that likely contribute to differential explant responsivity within species and conclude that in vitro shoot organogenesis occurs at the intersection between (epi) genetics, endogenous hormone levels, and environmental influences.

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