Thidiazuron-induced shoot organogenesis and production of hepatoprotective lignan phyllanthin and hypophyllanthin in Phyllanthus amarus

Alfalfa seeds of Cimarron VR, CW1446, CW2440, C94-118, C94-785, and WL311 were used as explants. BA, zeatin, and TDZ were evaluated on callus initiation, development, and shoot production. Callus initiation and development toward shoot organogenesis were enhanced when BA was added in the culture medium. Calli produced from BA treatments were compact, solid, and dark green. Similar results were obtained when zeatin was added in the culture medium. However, no shoots were produced from such calli. Multiple shoots were produced directly from each individual explant when TDZ was added to culture medium. However, when higher concentration of TDZ was used, number of shoots per explant was decreased, and dwarf shoots were produced. No apparent difference on shoot production was observed among the cultivars tested so far. Data on number of shoots per explant from two of these cultivars need to be statistically analyzed.

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Suppressive Effects of the Standardized Extract of Phyllanthus amarus on Type II Collagen-induced Rheumatoid Arthritis in Sprague Dawley Rats

Current Pharmaceutical Biotechnology ◽

10.2174/1389201020666181211124954 ◽

2019 ◽

Vol 19 (14) ◽

pp. 1156-1169

Author(s):

Javaid Alam ◽

Ibrahim Jantan ◽

Endang Kumolosasi ◽

Mohd A. Nafiah ◽

Muhammed A. Mesaik

Keyword(s):

Rheumatoid Arthritis ◽

Type Ii Collagen ◽

Phyllanthus Amarus ◽

Type Ii ◽

Sprague Dawley ◽

Sprague Dawley Rats

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Ảnh hưởng của dịch chiết cây diệp hạ châu (Phyllanthus amarus Schum. et Phonn) đến chất lượng cá bớp phi lê (Rachycentron canadum) trong điều kiện bảo quản lạnh

Can Tho University Journal of Science ◽

10.22144/ctu.jsi.2020.062 ◽

2020 ◽

Vol 56(Aquaculture) ◽

pp. 255

Author(s):

Trần Minh Phú ◽

Huỳnh Thị Kim Duyên ◽

Nguyễn Lê Anh Đào ◽

Nguyễn Thị Như Hạ ◽

Nguyễn Quốc Thịnh ◽

...

Keyword(s):

Phyllanthus Amarus ◽

Rachycentron Canadum

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Mechanistic Studies of the Antiallergic Activity of Phyllanthus amarus Schum. & Thonn. and Its Compounds

Molecules ◽

10.3390/molecules26030695 ◽

2021 ◽

Vol 26 (3) ◽

pp. 695

Author(s):

Nur Zahirah Abd Rani ◽

Kok Wai Lam ◽

Juriyati Jalil ◽

Hazni Falina Mohamad ◽

Mohd Shukri Mat Ali ◽

...

Keyword(s):

High Performance ◽

Binding Assay ◽

Radioligand Binding ◽

Positive Control ◽

Phyllanthus Amarus ◽

H1 Receptor ◽

Antiallergic Activity ◽

Ketotifen Fumarate ◽

Antihistamine Activity ◽

Basophilic Leukemia

Phyllanthus amarus Schum. & Thonn. (Phyllanthaceae) is a medicinal plant that is commonly used to treat diseases such as asthma, diabetes, and anemia. This study aimed to examine the antiallergic activity of P. amarus extract and its compounds. The antiallergic activity was determined by measuring the concentration of allergy markers release from rat basophilic leukemia (RBL-2H3) cells with ketotifen fumarate as the positive control. As a result, P. amarus did not stabilize mast cell degranulation but exhibited antihistamine activity. The antihistamine activity was evaluated by conducting a competition radioligand binding assay on the histamine 1 receptor (H1R). Four compounds were identified from the high performance liquid chromatography (HPLC) analysis which were phyllanthin (1), hypophyllanthin (2), niranthin (3), and corilagin (4). To gain insights into the binding interactions of the most active compound hypophyllanthin (2), molecular docking was conducted and found that hypophyllanthin (2) exhibited favorable binding in the H1R binding site. In conclusion, P. amarus and hypophyllanthin (2) could potentially exhibit antiallergic activity by preventing the activation of the H1 receptor.

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Natural Variation in Plant Pluripotency and Regeneration

Plants ◽

10.3390/plants9101261 ◽

2020 ◽

Vol 9 (10) ◽

pp. 1261

Author(s):

Robin Lardon ◽

Danny Geelen

Keyword(s):

De Novo ◽

Shoot Organogenesis ◽

In Vitro Regeneration ◽

Relative Role ◽

Root Explants ◽

Experimental Systems ◽

Complex Process ◽

Insight Into ◽

Molecular Framework

Plant regeneration is essential for survival upon wounding and is, hence, considered to be a strong natural selective trait. The capacity of plant tissues to regenerate in vitro, however, varies substantially between and within species and depends on the applied incubation conditions. Insight into the genetic factors underlying this variation may help to improve numerous biotechnological applications that exploit in vitro regeneration. Here, we review the state of the art on the molecular framework of de novo shoot organogenesis from root explants in Arabidopsis, which is a complex process controlled by multiple quantitative trait loci of various effect sizes. Two types of factors are distinguished that contribute to natural regenerative variation: master regulators that are conserved in all experimental systems (e.g., WUSCHEL and related homeobox genes) and conditional regulators whose relative role depends on the explant and the incubation settings. We further elaborate on epigenetic variation and protocol variables that likely contribute to differential explant responsivity within species and conclude that in vitro shoot organogenesis occurs at the intersection between (epi) genetics, endogenous hormone levels, and environmental influences.

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Optimisation of microwave-assisted extraction from Phyllanthus amarus for phenolic compounds-enriched extracts and antioxidant capacity

Chemical Papers ◽

10.1515/chempap-2016-0009 ◽

2016 ◽

Vol 70 (6) ◽

Cited By ~ 10

Author(s):

Van T. Nguyen ◽

Michael C. Bowyer ◽

Ian A. van Altena ◽

Christopher J. Scarlett

Keyword(s):

Phenolic Compounds ◽

Antioxidant Capacity ◽

Bioactive Compounds ◽

Pharmacological Activity ◽

Extraction Efficiency ◽

Phyllanthus Amarus ◽

Microwave Assisted Extraction ◽

Microwave Assisted ◽

Assisted Extraction ◽

Extraction Parameters

Abstractis known as a healing herb which has traditionally been used in the treatment of various diseases such as hepatitis, diabetes and cancer. The extraction parameters have great effects on the extraction efficiency of bioactive compounds and pharmacological activity of the extracts. This study sought to optimise the microwave-assisted extraction parameters for phenolic compounds-enriched extracts and antioxidant capacity from

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