A one-step real-time reverse transcription-polymerase chain reaction detection of classical swine fever virus using a minor groove binding probe

2010 ◽  
Vol 34 (4) ◽  
pp. 359-369 ◽  
Author(s):  
Guoyuan Wen ◽  
Jun Yang ◽  
Qingping Luo ◽  
Zhibin Hu ◽  
Nianhua Song ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Minor Groove Binding ◽  
Polymerase Chain Reaction Detection ◽  
Groove Binding ◽  
Chain Reaction ◽  
One Step ◽  
Polymerase Chain ◽  
A Minor

Differentiation of classical swine fever virus from ruminant pestiviruses by reverse transcription and polymerase chain reaction (RT-PCR)

1996 ◽  
Vol 48 (3-4) ◽  
pp. 373-379 ◽  
Author(s):  
Cláudio Wageck Canal ◽  
Isidro Hotzel ◽  
Laura Lopes de Almeida ◽  
Paulo Michel Roehe ◽  
Aoi Masuda
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Differentiation of virulent Shimen and vaccine C strains of classical swine fever virus by duplex reverse-transcription polymerase chain reaction

2017 ◽  
Vol 31 (5) ◽  
pp. 880-885
Author(s):  
Hai-min Li ◽  
Zi-yin Zhao ◽  
Kang-kang Guo ◽  
Yan-fen Jiang ◽  
Wei-min Zhang ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcription ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Detection of classical swine fever virus by reverse transcriptase-polymerase chain reaction and molecular hybridization

1997 ◽  
Vol 13 (3) ◽  
pp. 239-244
Author(s):  
S. D. Kirilenko ◽  
O. M. Deriabin ◽  
O. G. Deriabina ◽  
O. L. Kirilenko ◽  
Ju. O. Cherednik ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Molecular Hybridization ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Detection of Classical Swine Fever Virus in Boar Semen by Reverse Transcription–Polymerase Chain Reaction

2003 ◽  
Vol 15 (1) ◽  
pp. 35-41 ◽  
Author(s):  
Changsun Choi ◽  
Chanhee Chae
Keyword(s):  
Polymerase Chain Reaction ◽  
Nucleic Acid ◽  
Reverse Transcription ◽  
Classical Swine Fever Virus ◽  
Seminal Fluid ◽  
Classical Swine Fever ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Boar Semen ◽  
Polymerase Chain

A seminested reverse transcription–polymerase chain reaction (RT-PCR) was developed for the detection of classical swine fever virus (CSFV) in semen. Five boars were inoculated intranasally with CSFV isolate propagated in PK15 cells. Two boars inoculated with the supernatant of noninfected PK15 cells were kept as controls. Semen and serum samples were collected twice weekly for 63 days postinoculation (dpi). Samples were tested for the presence of antibodies to CSFV by an enzyme-linked immunosorbent assay and for the presence of CSFV nucleic acid by seminested RT-PCR. Antibodies to CSFV could be detected as early as 7 dpi in 1 boar, and all 5 infected boars were found positive by 14 dpi. CSFV from boar semen was infrequently identified by virus isolation compared with seminested RT-PCR. CSFV nucleic acid was detected in semen by seminested RT-PCR as early as 7 dpi in 3 infected boars and persistently thereafter in all 5 infected boars until 63 dpi. When separated fractions of CSFV-contaminated semen were analyzed by the seminested RT-PCR, the CSFV nucleic acid was detected mainly in seminal fluid and occasionally in nonsperm cells. CSFV antigen was also detected in nonsperm cells from semen smear by immunohistochemistry. Thus, infection via semen, specially through CSFV-infected seminal fluid, seems to be a major route of transmission of CSFV.

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