Molecular analysis of the hexon, penton base, and fiber-2 genes of Korean fowl adenovirus serotype 4 isolates from hydropericardium syndrome-affected chickens

AbstractFowl adenovirus serotype 4 (FAdV-4), the causative agent of hepatitis-hydropericardium syndrome (HHS), distributed widely in the poultry farms in China. Hexon is one of the major capsid proteins associated with viral species or serotypes. However, the epitopes of Hexon protein remain largely unknown. In this study, a monoclonal antibody (mAb) specific to Hexon protein of FAdV-4, designated as 3G8, was generated. Subsequently, the linear peptide recognized by 3G8 was mapped and identified as 213AYGAYVK219 using a series of overlapping peptides generated from Hexon protein. Amino acid sequence analysis revealed that the epitope recognized by 3G8 was highly conserved across all the FAdVs. The epitope was immunogenic and could be recognized by FAdV-4 positive chicken serum samples. These findings will enrich our knowledge regarding the epitope on Hexon and provide valuable information for further characterization of the antigenicity of Hexon protein.

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Pathogenesis of Hypervirulent Fowl Adenovirus Serotype 4: The Contributions of Viral and Host Factors

Viruses ◽

10.3390/v11080741 ◽

2019 ◽

Vol 11 (8) ◽

pp. 741 ◽

Cited By ~ 3

Author(s):

Zeng Wang ◽

Jun Zhao

Keyword(s):

Host Defense ◽

Host Factors ◽

Present Review ◽

Defense System ◽

Adenovirus Serotype ◽

Vaccine Candidates ◽

Hydropericardium Syndrome ◽

Fowl Adenovirus ◽

Host Defense System ◽

Fowl Adenovirus Serotype 4

Since 2015, severe outbreaks of hepatitis-hydropericardium syndrome (HHS), caused by hypervirulent fowl adenovirus serotype 4 (FAdV-4), have emerged in several provinces in China, posing a great threat to poultry industry. So far, factors contributing to the pathogenesis of hypervirulent FAdV-4 have not been fully uncovered. Elucidation of the pathogenesis of FAdV-4 will facilitate the development of effective FAdV-4 vaccine candidates for the control of HHS and vaccine vector. The interaction between pathogen and host defense system determines the pathogenicity of the pathogen. Therefore, the present review highlights the knowledge of both viral and host factors contributing to the pathogenesis of hypervirulent FAdV-4 strains to facilitate the related further studies.

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Penton-dodecahedron of fowl adenovirus serotype 4 as a vaccine candidate for the control of related diseases

10.1101/394072 ◽

2018 ◽

Author(s):

Qiuxia Tang ◽

Ruyi Dangi ◽

Li Qiu ◽

Zengqi Yang ◽

Xinglong Wang

Keyword(s):

Immune Response ◽

Recombinant Proteins ◽

Vaccine Antigen ◽

Penton Base ◽

Adenovirus Serotype ◽

Vaccine Candidates ◽

Fowl Adenovirus ◽

Infected Cells ◽

High Level ◽

Fowl Adenovirus Serotype 4

AbstractIn some serotypes of adenovirus (Ad), the penton base protein and attached trimeric fiber assemble into dodecameric virus-like particles called penton-dodecahedron (Pt-Dd), which can internalize into cells and can be used to deliver the vaccine antigen and drugs. Fowl adenovirus serotype 4 (FAdV-4) is an important poultry pathogens and causing seriously economic lost to poultry industry in China and several other counties. The produce of Pt-Dd in FAdV-4 infected cells as well as in those infected with the recombinant human Ad expressing fiber-1, fiber-2, and penton base was determine by Transmission electron microscopy (TEM). For the First time, we proved that FAdV-4 produced Pt-Dd in infected cells, which can also be assembled by the overexpressed recombinant proteins fiber-1, fiber-2, and penton base. Pt-Dd, as well as the recombinant proteins fiber-1, fiber-2, and penton base, were then used to immunize chickens. The humoral immune response, cell mediate immune response (CMI), and challenge results were used to evaluate the immune efficacy of the vaccine candidates. Pt-Dd induced the highest level of enzyme-linked immunosorbent assay antibodies and high levels of CMI, showing a significantly (p < 0.05) high level of interferon γ, interleukin-4, and major histocompatibility complex II expressions in peripheral blood mononuclear cells at 48 h post-infection. The challenge results showed that Pt-Dd, inactivated FAdV-4 vaccine, as well as fiber-1 induced the best protection (100%), followed by fiber-2 (80%) and penton (67%). The present study showed that FAdV-4-Pt-Dd and recombinant fiber-1 are good FAdV-4 vaccine candidates and could be used to replace the tissue-sourced inactivated FAdV-4 vaccine.ImportanceFAdV-4-Pt-Dds were discovered in FAdV-4 infected cells, and which were also assembled in cells transduced with recombinant human adenovirus expressing fiber-1, fiber-2, and penton base. FAdV-4-Pt-Dds internalize into cells with high efficiency, so that it can be used for delivery vaccine antigen or drugs. Immunization chickens with Pt-Dd and fiber-1 obtained by transduction HEK-293T cells induced significant high level humoral and cellular mediated immune responses, and also 100% challenge protection in chickens indicating that they are good FAdV-4 vaccine candidates. What more, the Pt-Dd obtained by transduction HEK-293T cell would have no DNA and adenovirus contamination as adenovirus could not package in HEK-293T cells.

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Complete genome sequence and pathogenicity of fowl adenovirus serotype 4 involved in hydropericardium syndrome in Southwest China

Microbial Pathogenesis ◽

10.1016/j.micpath.2018.02.012 ◽

2018 ◽

Vol 117 ◽

pp. 290-298 ◽

Cited By ~ 15

Author(s):

Ru Guan ◽

Yiming Tian ◽

Xiaoxiao Han ◽

Xin Yang ◽

Hongning Wang

Keyword(s):

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Southwest China ◽

Adenovirus Serotype ◽

Hydropericardium Syndrome ◽

Fowl Adenovirus ◽

Fowl Adenovirus Serotype 4

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Hydropericardium syndrome caused by fowl adenovirus serotype 4 in replacement pullets

Journal of Veterinary Medical Science ◽

10.1292/jvms.18-0168 ◽

2019 ◽

Vol 81 (2) ◽

pp. 245-251 ◽

Cited By ~ 2

Author(s):

Zhen CHEN ◽

Shaohua SHI ◽

Baoming QI ◽

Su LIN ◽

Cuiteng CHEN ◽

...

Keyword(s):

Adenovirus Serotype ◽

Hydropericardium Syndrome ◽

Fowl Adenovirus ◽

Fowl Adenovirus Serotype 4

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Requirement of Cellular Protein CCT7 for the Replication of Fowl Adenovirus Serotype 4 (FAdV-4) in Leghorn Male Hepatocellular Cells Via Interaction with the Viral Hexon Protein

Viruses ◽

10.3390/v11020107 ◽

2019 ◽

Vol 11 (2) ◽

pp. 107 ◽

Cited By ~ 2

Author(s):

Junfeng Gao ◽

Mingliang Zhao ◽

Xueyan Duan ◽

Yongqiang Wang ◽

Hong Cao ◽

...

Keyword(s):

Molecular Mechanisms ◽

Ectopic Expression ◽

Cellular Protein ◽

Economic Losses ◽

Adenovirus Serotype ◽

Hydropericardium Syndrome ◽

Fowl Adenovirus ◽

Infected Cells ◽

Lmh Cells ◽

Fowl Adenovirus Serotype 4

Fowl adenovirus serotype 4 (FAdV-4) causes hepatitis-hydropericardium syndrome (HHS), leading to severe economic losses in the poultry industry. Although the pathogenesis of FAdV-4 infection has caused much attention, the underlying molecular mechanisms remain poorly understood. Here, we identified chaperonin containing TCP-1 subunit eta (CCT7) as an interacting partner of the FAdV-4 capsid protein hexon. We found that ectopic expression of CCT7 in leghorn male hepatocellular (LMH) cells enhanced hexon expression in pRK5-flag-hexon transfected cells. On the contrary, knockdown of cellular CCT7 by RNAi markedly reduced hexon expression in FAdV-4-infected cells and suppressed viral replication. These data suggest that CCT7 is required for FAdV-4 replication and may serve as a potential target for controlling FAdV-4 infection.

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