Immobilized cells cultivated in semi-continuous mode in a fluidized bed reactor for xylitol production from sugarcane bagasse

2005 ◽  
Vol 21 (4) ◽  
pp. 531-535 ◽  
Author(s):  
J. C. Santos ◽  
S. S. Silva S. I. Mussatto ◽  
W. Carvalho ◽  
M. A. A. Cunha
2008 ◽  
Vol 19 (4) ◽  
pp. 1210-1215 ◽  
Author(s):  
J. C. Santos ◽  
W. Carvalho ◽  
S. S. Silva ◽  
A. Converti

2005 ◽  
Vol 40 (1) ◽  
pp. 113-118 ◽  
Author(s):  
Júlio C. Santos ◽  
Attilio Converti ◽  
Walter de Carvalho ◽  
Solange I. Mussatto ◽  
Silvio S. da Silva

2000 ◽  
Vol 55 (3-4) ◽  
pp. 213-217 ◽  
Author(s):  
Walter de Carvalho ◽  
Silvio Silvério da Silva ◽  
Michele Vitolo ◽  
Ismael Maciel de Mancilha

Abstract In this study we used the yeast Candida guilliermondii FTI 20037 immobilized by entrapment in Ca-alginate beads (2 .5 -3 mm diameter) for xylitol production from concentrated sugarcane bagasse hemicellulosic hydrolysate in a repeated batch system. The fermentation runs were carried out in 125- and 250-ml Erlenmeyer flasks placed in an orbital shaker at 30 °C and 200 rpm during 72 h, keeping constant the proportion between work volume and flask total volume. According to the results, cell viability was substantially high (98%) in all fermentative cycles. The values of parameters xylitol yield and volumetric productivity increased significantly with the reutilization of the immobilized biocatalysts. The highest values of xylitol final concentration (11.05 g/1), yield factor (0.47 gig) and volumetric productivity (0.22 g/lh) were obtained in 250-ml Erlenmeyer flasks containing 80 ml of medium plus 20 mi of immobilized biocatalysts. The support used in this study (Ca-alginate) presented stability in the experimental conditions used. The results show that the use of immobilized cells is a promising approach for increasing the xylitol production rates.


2020 ◽  
Vol 12 (4) ◽  
pp. 043102
Author(s):  
Marcelo Ricardo Queiroz Medeiros ◽  
Manuel Ernani Cruz ◽  
Albino José Kalab Leiroz ◽  
Rafaela Frota Reinaldo ◽  
Gabriel Lisbôa Verissimo

2010 ◽  
Vol 62 (4) ◽  
pp. 947-955 ◽  
Author(s):  
Xiao-ming Li ◽  
Qi Yang ◽  
Ying Zhang ◽  
Wei Zheng ◽  
Xiu Yue ◽  
...  

The performance of a fluidized bed reactor using immobilized Phanerochaete chrysosporium to remove 2,4-dichlorophenol (2,4-DCP) from aqueous solution was investigated. The contribution of lignin peroxidase (LiP) and manganese peroxidase (MnP) secreted by Phanerochaete chrysosporium to the 2,4-DCP degradation was examined. Results showed that Lip and Mnp were not essential to 2,4-DCP degradation while their presence enhanced the degradation process and reaction rate. In sequential batch experiment, the bioactivity of immobilized cells was recovered and improved during the culture and the maximum degradation rate constant of 13.95 mg (Ld)−1 could be reached. In continuous bioreactor test, the kinetic behavior of the Phanerochaete chrysosporium immobilized on loofa sponge was found to follow the Monod equation. The maximum reaction rate was 7.002 mg (Lh)−1, and the saturation constant was 26.045 mg L−1.


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