Targeted disruption of a G protein α subunit gene results in reduced growth and pathogenicity in Rhizoctonia solani

2007 ◽  
Vol 24 (3) ◽  
pp. 345-351 ◽  
Author(s):  
Kanlayani Charoensopharat ◽  
Nuntipa Aukkanit ◽  
Sudarat Thanonkeo ◽  
Weerasak Saksirirat ◽  
Pornthap Thanonkeo ◽  
...  
Keyword(s):  
Rhizoctonia Solani ◽  
G Protein ◽  
Subunit Gene ◽  
Targeted Disruption ◽  
Α Subunit ◽  
G Protein Α Subunit

Targeted disruption of a G protein α subunit gene results in reduced pathogenicity in Fusarium oxysporum

2002 ◽  
Vol 41 (6) ◽  
pp. 407-413 ◽  
Author(s):  
Sona Jain ◽  
Kouichi Akiyama ◽  
Kenjiro Mae ◽  
Tomizo Ohguchi ◽  
Renkichi Takata
Keyword(s):  
Fusarium Oxysporum ◽  
G Protein ◽  
Subunit Gene ◽  
Targeted Disruption ◽  
Α Subunit ◽  
G Protein Α Subunit

scholarly journals Targeted Disruption of a Fungal G-Protein β Subunit Gene Results in Increased Vegetative Growth but Reduced Virulence

1997 ◽  
Vol 10 (8) ◽  
pp. 984-993 ◽  
Author(s):  
Shin Kasahara ◽  
Donald L. Nuss
Keyword(s):  
Amino Acid ◽  
G Protein ◽  
Vegetative Growth ◽  
Synthetic Medium ◽  
Cryphonectria Parasitica ◽  
Β Subunit ◽  
Subunit Gene ◽  
Targeted Disruption ◽  
Fungal Virulence ◽  
Α Subunit

Targeted disruption of two G-protein α subunit genes in the chestnut blight fungus Cryphonectria parasitica revealed roles for the Giα subunit CPG-1 in fungal reproduction, virulence, and vegetative growth. A second Gα subunit, CPG-2, was found to be dispensable for these functions. We now report the cloning and targeted disruption of a C. parasitica G-protein β subunit gene. The deduced amino acid sequence encoded by this gene, designated cpgb-1, was found to share 66.2, 65.9, and 66.7% amino acid identity with Gβ homologues from human, Drosophila, and Dictyostelium origins, respectively, but only 39.7% identity with the Saccharomyces cerevisiae Gβ homologue STE4 product. Low stringency Southern hybridization failed to detect any related Gβ subunit genes in C. parasitica. Targeted disruption of cpgb-1 resulted in several of the changes previously reported to accompany disruption of the C. parasitica Giα subunit gene cpg-1. These included very significant reductions in pigmentation, asexual sporulation, and virulence. In contrast to results obtained for Giα gene disruption, the reduction in virulence resulting from the disruption of a Gβ gene was accompanied by increased, rather than decreased, vegetative growth on synthetic medium. The relevance of these results to mechanisms of fungal virulence is considered.

Regulated Expression of the Arabidopsis G Protein α Subunit Gene GPA1

1994 ◽  
Vol 155 (1) ◽  
pp. 3-14 ◽  
Author(s):  
Hai Huang ◽  
Catherine A. Weiss ◽  
Hong Ma
Keyword(s):  
G Protein ◽  
Subunit Gene ◽  
Α Subunit ◽  
Regulated Expression ◽  
G Protein Α Subunit

scholarly journals Mutations in the Cone Photoreceptor G-Protein α-Subunit Gene GNAT2 in Patients with Achromatopsia

2002 ◽  
Vol 71 (2) ◽  
pp. 422-425 ◽  
Author(s):  
Susanne Kohl ◽  
Britta Baumann ◽  
Thomas Rosenberg ◽  
Ulrich Kellner ◽  
Birgit Lorenz ◽  
...  
Keyword(s):  
G Protein ◽  
Cone Photoreceptor ◽  
Subunit Gene ◽  
Α Subunit ◽  
G Protein Α Subunit
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