Production, purification and characterization of cholesterol oxidase from a newly isolated Streptomyces sp.

2013 ◽  
Vol 29 (11) ◽  
pp. 2077-2085 ◽  
Author(s):  
Ram Niwas ◽  
Vineeta Singh ◽  
Rajbir Singh ◽  
Divya Tripathi ◽  
C. K. M. Tripathi
Keyword(s):  
Cholesterol Oxidase ◽  
Purification And Characterization ◽  
Streptomyces Sp

scholarly journals Partial purification and characterization of an endo-α-N-acetylgalactosaminidase from the culture medium of Streptomyces sp. OH-11242

1992 ◽  
Vol 288 (2) ◽  
pp. 475-482 ◽  
Author(s):  
I Ishii-Karakasa ◽  
H Iwase ◽  
K Hotta ◽  
Y Tanaka ◽  
S Omura
Keyword(s):  
Enzyme Activity ◽  
Culture Medium ◽  
Gel Filtration ◽  
Partial Purification ◽  
Gastric Mucus ◽  
Purification And Characterization ◽  
Streptomyces Sp ◽  
Optimum Ph ◽  
New Type

For the purification of a new type of endo-alpha-N-acetylgalactosaminidase from the culture medium of Streptomyces sp. OH-11242 (endo-GalNAc-ase-S) [Iwase, Ishii, Ishihara, Tanaka, Omura & Hotta (1988) Biochem. Biophys. Res. Commun. 151, 422-428], a method for assaying enzyme activity was established. Using purified pig gastric mucus glycoprotein (PGM) as the substrate, oligosaccharides liberated from PGM were pyridylaminated, and the reducing terminal sugars of oligosaccharides larger than Gal beta 1-3GalNAc were analysed by h.p.1.c. The crude enzyme of endo-GalNAc-ase-S was prepared as an 80% (w/v) ammonium sulphate precipitate from the concentrated culture medium. The enzyme was partially purified by gel chromatofocusing and subsequent DEAE-Toyopearl chromatography. Endo-enzyme activity eluted around pI 4.8 on a gel chromatofocusing column and eluted with 0.19-0.25 M-NaCl on a DEAE-Toyopearl column. In the enzyme fraction obtained, no exo-glycosidases or proteases could be detected. The molecular mass of the enzyme was estimated as 105 kDa by gel filtration, and the optimum pH was 5.5. Endo-GalNAc-ase-S hydrolysed the O-glycosidic linkage between GalNAc and Ser (Thr) in 3H-labelled and unlabelled asialofetuin, liberating both the disaccharide (Gal beta 1-3GalNAc) and the tetrasaccharide [Gal beta 1-3 (Gal beta 1-4GlcNAc beta 1-6)GalNAc]. When endo-alpha-N-acetylgalactosaminidase from Alcaligenes sp. (endo-GalNac-ase-A) was incubated with 3H-labelled and unlabelled asialofetuin, only the disaccharide (Gal beta 1-3GalNAc) was liberated.

Purification and Characterization of Extra-Cellular Cholesterol Oxidase From Rhodococcus sp. PTCC 1633

2008 ◽  
Vol 7 (4) ◽  
pp. 751-756 ◽  
Author(s):  
M.T. Yazdi ◽  
Z.T. Yazdi ◽  
A. Ghasemian ◽  
G. Zarrini ◽  
N.H. Olyaee ◽  
...  
Keyword(s):  
Cholesterol Oxidase ◽  
Purification And Characterization ◽  
Cellular Cholesterol ◽  
Rhodococcus Sp

Purification and characterization of a fibrinolytic enzyme from Streptomyces sp. XZNUM 00004

2012 ◽  
Vol 28 (7) ◽  
pp. 2479-2486 ◽  
Author(s):  
Xiuyun Ju ◽  
Xiaoying Cao ◽  
Yong Sun ◽  
Zhe Wang ◽  
Chengliang Cao ◽  
...  
Keyword(s):  
Fibrinolytic Enzyme ◽  
Purification And Characterization ◽  
Streptomyces Sp
Sign in / Sign up

Export Citation Format

Share Document