Produksi Enzim Fibrinolitik Tempe oleh Rhizopus oryzae FNCC 6078

ackground Rhizopus oryzae FNCC 6078 had been evaluated producing fibrinolytic enzyme under solid state fermentation. Soybean had been used to produce fibrinolytic enzyme through fermentation in tempeh. The main purpose of this study was to reveal optimum condition for fermentation. The parameters of the condition were inoculum volume, incubation period and temperature. Optimum condition was defined by maximum fibrinolytic activity. Methode Fibrinolytic activity was measured using spectrophotometry at 274 nm. Result optimum condition for producing fibrinolytic enzyme was 1,5 mL volume of inoculum of Rhizopus oryzae suspension in 25%T, 42 hours for incubation period and 35oC temperature incubation.

Fibrin-derived Peptide Bβ15-42 and Earthworm Fibrinolytic Enzyme A Inhibit Atherosclerosis and Inflammation via the VE-cadherin Pathway

Elevated fibrinogen increases risk for cardiovascular and cerebrovascular diseases. The biological effects of the fibrin-derived peptide Bβ15–42 are different from fibrin and promote vascular and anti-inflammatory effects. Fragments of fibrinogen cleavage by earthworm fibrinolytic enzyme A (EFEa) are structurally similar to Bβ15–42. Therefore, we evaluated if Bβ15–42 and EFEa have anti-atherosclerotic effects. To investigate the anti-atherosclerotic effect of Bβ15–42 and EFEa, we used New Zealand rabbits fed a high cholesterol diet to promote atherosclerosis. Human umbilical vascular endothelial cells with high expression of VE-cadherin were used to determine the mechanism of action of Bβ15–42 and EFEa to inhibit the deleterious effect of fibrin. Both Bβ15–42 and EFEa significantly reduced atherosclerosis in rabbits fed a high cholesterol diet. Vascular fibrinogen deposition and inflammatory cell aggregation was significantly reduced in rabbits treated with Bβ15–42 or EFEa. In addition, Bβ15–42 and EFEa stabilized the structure of endothelial cells and decreased inflammatory cell migration. Bβ15–42 and EFEa protected endothelial cell function by reducing the effect of fibrinogen in the VE-cadherin pathway. Therefore, the fibrin-derived Bβ15–42 peptide exhibited anti-atherosclerotic effects and reduced vascular fibrinogen deposition and inflammatory cell aggregation in the aorta. Furthermore, EFEa has similar Bβ15-42-like anti-atherosclerotic effects.

Optimization of Cultural Conditions for Maximum Production of Fibrinolytic Enzymes from the Local Marine Bacterial Isolates and Evaluation of their Wound Healing and Clot Dissolving Properties

Fibrinolytic enzymes find necessary applications to treat and prevent cardiovascular diseases. In this study, optimal conditions for enhancing the production of fibrinolytic enzyme from local marine bacterial strains were evaluated. The present study also focuses on screening of wound healing efficacy of the isolated fibrinolytic enzymes.Various physical parameters such as temperature, pH, incubation time and medium components viz. inoculum size, substrate (nitrogen and carbon) concentrations were optimized. A cultivation medium was designed using optimized conditions for mass production of fibrinolytic enzyme and specific activity of enzyme was analyzed. The maximum enzyme production was observed at 37 °C temperature, 8.0 pH,substrate concentration with 3 ml inoculum size and 32 h. of incubation time. Among the different carbon sources tested, Mannitol showed maximum enzyme activity i.e 538 U/ml. yeast extract was found to be the best nitrogen source with an enzyme activity of 498 U/ml. The best substrate for the production fibrinolytic enzyme was found to be kernelwith high activity of 1056U/ml. The crude enzyme displayed potent activity and digested blood clot completely in in vitro condition and exhibited potent activity on wound healing property in macrophages.

Isolation, production and application of fibrinolytic enzyme from fermented food sources

The accumulation of the fibrin in bacterial fibrinolytic enzymes finds applications to treat and prevent cardiovascular diseases which fail in hemostasis that leads to the formation of undesirable blood clots in the blood vessels leading to condition called thrombosis. The fibrinolytic enzymes from food grade organisms are useful for thrombolytic therapy. Conventional thrombolytic agents such as streptokinase, nattokinase etc. Nattokinase is one such fibrinolytic enzyme with a wide range of applications in Pharmaceutical industry, health care and medicine etc. Hence, potent blood-clot dissolving protein used for the treatment of cardiovascular diseases is produced by the bacterium Bacillus subtilis during the fermentation of soybeans to produce Natto. The health benefits of some fermented foods are synthesis of nutrients, prevention of cancer, diabetes due to presence of functional microorganisms, which possess probiotics properties, antimicrobial, antioxidant, etc. The first report of fibrinolytic enzyme production of cow dung used as a cheap substrate from Bacillus species in SSF has been given earlier. This review describes different isolation methods which enable the screening and selection of promising organisms for industrial production. The purification and properties of these fibrinolytic proteases is discussed, and the use of fibrinolytic enzyme. In order to obtain Bacillus species producing fibrinolytic enzymes, the fermented food sample such as sprouted grain and processed grain etc were used. The heat tolerant isolates initially were selected for catalase test. Fibrinolytic activity of the selected isolates was determined by using Fibrin plate assay. From the above work, it can be concluded that the fibrinolytic enzyme produced by Bacillus from fermented food samples had the ability to degrade the fibrin and hence can be used for functional food formulation.