DNA authentication of So-jutsu (Atractylodes lancea rhizome) and Byaku-jutsu (Atractylodes rhizome) obtained in the market based on the nucleotide sequence of the 18S–5.8S rDNA internal transcribed spacer region

2006 ◽  
Vol 60 (2) ◽  
pp. 149-156 ◽  
Author(s):  
Yahong Guo ◽  
Kenji Kondo ◽  
Susumu Terabayashi ◽  
Yutaka Yamamoto ◽  
Hiroshi Shimada ◽  
...  
1992 ◽  
Vol 20 (1) ◽  
pp. 161-162 ◽  
Author(s):  
K. H. Asay ◽  
C. Hsiao ◽  
N. J. Chatterton ◽  
K. B. Jensen ◽  
R. R-C. Wang

1997 ◽  
Vol 75 (5) ◽  
pp. 693-698 ◽  
Author(s):  
Richard C. Hamelin ◽  
Jimmy Rail

Sequences in the 5.8S rDNA and internal transcribed spacer region were obtained for taxa belonging to Gremmeniella spp., and a phylogenetic analysis was carried out using parsimony and distance methods. A set of hypotheses concerning evolutionary relationships between members of that genus was tested. The hypothesis that all members of Gremmeniella abietina var. abietina are closely related was rejected. It appears that the Asian race is quite divergent from the European and North American races, but could be more closely related to G. abietina var. balsamea. The phylogeny was consistent with host specificity: members of G. abietina var. abietina generally do not infect Abies or Picea spp. but the Asian race occurs on Abies sachalinensis in Japan. The hypothesis that host specialization has created divergence within G. abietina var. balsamea was supported by our analysis because the levels of divergence between isolates originating from Picea spp. and Abies spp. were similar to those found between the North American and European races of G. abietina var. abietina. The hypothesis that greater divergence occurred between Gremmeniella abietina and Gremmeniella laricina than between G. abietina var. abietina and G. abietina var. balsamea was not supported. The level of divergence was as large between the two varieties within G. abietina as between the two species G. abietina and G. laricina. These results indicate that similar taxonomic levels should be applied to these three taxa. Key words: scleroderris canker, internal transcribed spacer (ITS).


1992 ◽  
Vol 20 (1) ◽  
pp. 159-160 ◽  
Author(s):  
N. J. Chatterton ◽  
C. Hsiao ◽  
K. H. Asay ◽  
R. R-C Wang ◽  
K. B. Jensen

1992 ◽  
Vol 20 (1) ◽  
pp. 165-166 ◽  
Author(s):  
N. J. Chatterton ◽  
C. Hsiao ◽  
K. H. Asay ◽  
K. B. Jensen ◽  
R. R-C. Wang

Genome ◽  
1997 ◽  
Vol 40 (4) ◽  
pp. 479-486 ◽  
Author(s):  
S. Bekal ◽  
J. P. Gauthier ◽  
R. Rivoal

This study examined the restriction polymorphism (RFLP) of the nuclear ribosomal DNA in Heterodera avenae, H. filipjevi, H. mani, H. latipons, and the taxonomically unclear Gotland strain in order to establish a molecular characterization and phylogenetic relationships in the complex of cereal cyst nematodes (CCN). The internal transcribed spacer (ITS) and 5.8S rDNA were amplified by PCR from a single female or a cyst of 27 different geographic isolates of the CCN complex and one population of H. schachtii, used as outgroup. The amplified product was 1.2 kb long and 14 of 15 enzymes produced restriction fragments for each isolate. Relationships between populations were determined from UPGMA analysis based on distance values calculated from RFLP data. Digestions with TaqI clearly differentiated H. avenae, H. latipons, and a group composed of H. filipjevi and the Gotland strain. Six endonucleases (HaeIII, HinfI, ItaI, PstI, TaqI, and Tru9I) produced the same restriction pattern with H. filipjevi and the Gotland strain, and both were clearly separated from H. avenae with PstI. Restriction sites have revealed a mixture of the species H. latipons and H. avenae, and possible infraspecific variation in H. avenae. The inferred phylogenetic relationships of species in the CCN complex are in agreement with their morphological characterization.Key words: cereal cyst nematodes, Heterodera avenae, PCR, RFLP, ribosomal diversity.


1992 ◽  
Vol 20 (1) ◽  
pp. 163-164 ◽  
Author(s):  
N. J. Chatterton ◽  
C. Hsiao ◽  
K. H. Asay ◽  
K. B. Jensen ◽  
R. R-C. Wang

Planta Medica ◽  
2014 ◽  
Vol 80 (10) ◽  
Author(s):  
P Jiao ◽  
J Tseng-Crank ◽  
B Corneliusen ◽  
M Yimam ◽  
M Hodges ◽  
...  

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