Combined pluronic P85- and ultrasound contrast agents-mediated gene transfection to HepG2 cells

2011 ◽  
Vol 31 (6) ◽  
pp. 842-845 ◽  
Author(s):  
Xijun Zhang ◽  
Kaiyan Li ◽  
Xian Cui ◽  
Liangjun Hu ◽  
Yunchao Chen
Keyword(s):  
Contrast Agents ◽  
Hepg2 Cells ◽  
Gene Transfection ◽  
Ultrasound Contrast Agents ◽  
Ultrasound Contrast ◽  
Pluronic P85

Preparation of Cationic Lipid-coated Ultrasound Contrast Agents and Noninvasive Gene Transfection Via Ultrasound-targeted Microbubble Destruction

2018 ◽  
Vol 24 (30) ◽  
pp. 3587-3595 ◽  
Author(s):  
Feng Yang ◽  
Yue Li ◽  
Chun Liufu ◽  
Yi Wang ◽  
ZhiYi Chen
Keyword(s):  
Thin Film ◽  
Contrast Agents ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Mechanical Vibration ◽  
Great Influence ◽  
Ultrasound Contrast Agents ◽  
Therapeutic Drug ◽  
Ultrasound Contrast ◽  
Ultrasound Parameters

Purpose: Ultrasound contrast agents involving a therapeutic drug applied during enhanced imaging can be used for targeted therapy. The preparation of contrast agents is a precondition and basis for the use of multifunctional contrast agents in molecular imaging. Methods: This study uses thin-film hydration-mechanical vibration to carry out the preliminary preparation of a cationic contrast agent (CCA); characterizes the particle diameter, potential, distribution, and concentration of the agents; and optimizes the factors affecting the preparation of the agents. Results: This study found that thin-film hydration-mechanical vibration methods offer a better preparation effectiveness and achieve smaller particle diameters and more even distributions, as well as give better imaging performance. Different concentrations of CCA and plasmid and different gene transfection methods can produce different degrees of sonoporation to achieve optimal transfection efficiency. Ultrasound parameters have a great influence on transfection efficiency and plasmid integrity. A previous study confirmed that the ultrasound parameters of 1 MHz, 1 W/cm2, a duty cycle controller (DC) of 20%, and irradiation for 1 min can well deliver genes to tumor cells, with little impact on cell survival. Conclusion: Our findings indicate that ultrasound-targeted microbubble destruction (UTMD)-mediated CCA destruction facilitates gene transfection and may represent an effective gene delivery method for cervical cancer therapy.

Ultrasound contrast agents: microbubbles made simple for the pediatric radiologist

2021 ◽  
Author(s):  
Anush Sridharan ◽  
John R. Eisenbrey ◽  
Flemming Forsberg ◽  
Norbert Lorenz ◽  
Ludwig Steffgen ◽  
...  
Keyword(s):  
Contrast Agents ◽  
Ultrasound Contrast Agents ◽  
Pediatric Radiologist ◽  
Ultrasound Contrast

scholarly journals New Applications in Echocardiography for Ultrasound Contrast Agents in the 21st Century

2020 ◽  
Vol 46 (5) ◽  
pp. 1071-1081 ◽  
Author(s):  
Thomas R. Porter ◽  
Steve B. Feinstein ◽  
Folkert J. Ten Cate ◽  
Annemien E. van den Bosch
Keyword(s):  
Contrast Agents ◽  
21St Century ◽  
Ultrasound Contrast Agents ◽  
Ultrasound Contrast ◽  
New Applications

Emerging Applications of Ultrasound-Contrast Agents in Radiation Therapy

2021 ◽  
Vol 47 (6) ◽  
pp. 1465-1474
Author(s):  
Quezia Lacerda ◽  
Mohamed Tantawi ◽  
Dennis B. Leeper ◽  
Margaret A. Wheatley ◽  
John R. Eisenbrey
Keyword(s):  
Radiation Therapy ◽  
Contrast Agents ◽  
Ultrasound Contrast Agents ◽  
Ultrasound Contrast
Sign in / Sign up

Export Citation Format

Share Document