Effect of phytohormones on fiber initiation of cotton ovule

Cotton (Gossypium hirsutum) fiber, an extremely elongated and thickened single cell of the seed epidermis, is the worlds most important natural and economical textile fiber. Unlike Arabidopsis leaf trichomes, fiber initials are randomly developed and frequently form in adjacent seed epidermal cells and follow no apparent pattern. Numerous publications suggested cotton fiber development shares a similar mechanism with Arabidopsis leaf trichome development. Here we show that H2O2 accumulation in cotton ovule epidermal cells by NBT staining ovules at different development stages between TM1 and N1n2, a lintless-fuzzless doubled mutant originated from TM1. In contrast, Arabidopsis and cotton leaf trichomes do not show H2O2 content. By adding DPI (H2O2 inhibitor) and SHAM (H2O2 activator) in vitro ovule cultures, we show fiber initiation directly involves with H2O2 accumulation. We propose that the directional accumulation of H2O2 in cotton ovule epidermal cell is the drive for fiber initiation, elongation.

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FIBER INITIATION ON THE COTTON OVULE (GOSSYPIUM HIRSUTUM)

American Journal of Botany ◽

10.1002/j.1537-2197.1975.tb14105.x ◽

1975 ◽

Vol 62 (7) ◽

pp. 723-730 ◽

Cited By ~ 55

Author(s):

James McD. Stewart

Keyword(s):

Gossypium Hirsutum ◽

Fiber Initiation ◽

Cotton Ovule

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Sucrose‐enhanced reactive oxygen species generation promotes cotton fiber initiation and secondary cell wall deposition

Plant Biotechnology Journal ◽

10.1111/pbi.13594 ◽

2021 ◽

Author(s):

Xiaoyan Ding ◽

Xianbi Li ◽

Lei Wang ◽

Jianyan Zeng ◽

Liang Huang ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Cell Wall ◽

Cotton Fiber ◽

Secondary Cell Wall ◽

Reactive Oxygen Species Generation ◽

Reactive Oxygen ◽

Oxygen Species ◽

Fiber Initiation ◽

Wall Deposition ◽

Cell Wall Deposition

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Cytokinin inhibits cotton fiber initiation by disrupting PIN3a-mediated asymmetric accumulation of auxin in the ovule epidermis

Journal of Experimental Botany ◽

10.1093/jxb/erz162 ◽

2019 ◽

Vol 70 (12) ◽

pp. 3139-3151 ◽

Cited By ~ 7

Author(s):

Jianyan Zeng ◽

Mi Zhang ◽

Lei Hou ◽

Wenqin Bai ◽

Xingying Yan ◽

...

Keyword(s):

Cotton Fiber ◽

Plasma Membranes ◽

Antagonistic Effect ◽

Fiber Cell ◽

In Planta ◽

Polar Localization ◽

Fiber Cells ◽

Fiber Initiation ◽

Fiber Cell Initiation

AbstractAuxin-dependent cell expansion is crucial for initiation of fiber cells in cotton (Gossypium hirsutum), which ultimately determines fiber yield and quality. However, the regulation of this process is far from being well understood. In this study, we demonstrate an antagonistic effect between cytokinin (CK) and auxin on cotton fiber initiation. In vitro and in planta experiments indicate that enhanced CK levels can reduce auxin accumulation in the ovule integument, which may account for the defects in the fiberless mutant xu142fl. In turn, supplementation with auxin can recover fiber growth of CK-treated ovules and mutant ovules. We further found that GhPIN3a is a key auxin transporter for fiber-cell initiation and is polarly localized to the plasma membranes of non-fiber cells, but not to those of fiber cells. This polar localization allows auxin to be transported within the ovule integument while specifically accumulating in fiber cells. We show that CKs antagonize the promotive effect of auxin on fiber cell initiation by undermining asymmetric accumulation of auxin in the ovule epidermis through down-regulation of GhPIN3a and disturbance of the polar localization of the protein.

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