In vitro plantlet regeneration and assessment of alkaloid contents from callus cultures of Ephedra foliata (Unth phog), a source of anti-asthmatic drugs

2014 ◽  
Vol 36 (11) ◽  
pp. 3071-3079 ◽  
Author(s):  
Deepika Lodha ◽  
Ashok Kumar Patel ◽  
Manoj K. Rai ◽  
N. S. Shekhawat
Keyword(s):  
Callus Cultures ◽  
Plantlet Regeneration ◽  
Ephedra Foliata ◽  
Alkaloid Contents

In vitro plantlet formation from flower petal explants of Hemerocallis cv. Chipper Cherry

1976 ◽  
Vol 54 (7) ◽  
pp. 616-618 ◽  
Author(s):  
Charles W. Heuser ◽  
Darrel A. Apps
Keyword(s):  
Acetic Acid ◽  
Callus Tissue ◽  
Callus Cultures ◽  
Plantlet Regeneration ◽  
Tissue Cultures ◽  
Flower Petal ◽  
Plantlet Formation ◽  
Yellowish Green ◽  
Dichlorophenoxy Acetic Acid

Plantlet regeneration has been induced from callus tissue cultures obtained from petal parts of Hemerocallis cv. Chipper Cherry. Callus cultures capable of regenerating whole plantlets were established on the agar-solidified Murashige and Skoog's medium supplemented with (2,4-dichlorophenoxy)acetic acid (2,4-D) (1.0 mg/litre) + kinetin (1.0 mg/litre). The callus formed was dense, yellowish-green in color, and had what appeared to be meristematic protuberances. Shoots and roots developed when the callus was subcultured on a medium lacking 2,4-D.

In Vitro Establishment of Powdery Mildew (Microsphaera pulchra) on Microshoots of Flowering Dogwood

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 506a-506
Author(s):  
L.A. Klein ◽  
M.T. Windham ◽  
R.N. Trigiano
Keyword(s):  
Powdery Mildew ◽  
Woody Plant ◽  
Infected Individual ◽  
Callus Cultures ◽  
Fungal Culture ◽  
Culture Collections ◽  
Flowering Dogwood ◽  
Plant Parasite ◽  
In Vitro Establishment

Microshoot and callus cultures of Cornus florida (flowering dogwood), which were grown on woody plant medium amended with BA, were inoculated with Microsphaera pulchra (an obligate plant parasite) by gently shaking infected leaves bearing numerous conidia over the tissue. Culture dishes were sealed with parafilm and incubated at 24 °C with 25 mol·m–2·s–1 provided by cool fluorescent bulbs for 15 h. Cultures were examined with a dissecting scope every 24 h and cultures transferred when contaminating fungi were present. Specimens were prepared light microscopy and SEM. The fungus infected individual callus cells, but did not sporulate. In contrast, powdery mildew was well-established (both primary and secondary hyphae) in 70% of the microshoot cultures after 6 days and sporulated on 20% by 7 to 8 days. The cellular relationship between host and pathogen in vitro was similar to that found in greenhouse-grown plants. This technique has possible applications in maintaining fungal culture collections and studying host–pathogen relationships under more stringently controlled conditions.

Comparative Investigation on Formation and Accumulation of Rare Phenylpropanoids in Plants and in vitro Cultures of Pimpinella major

1990 ◽  
Vol 45 (6) ◽  
pp. 602-606 ◽  
Author(s):  
B. Merkel ◽  
J. Reichling
Keyword(s):  
Callus Culture ◽  
Nutrient Medium ◽  
Callus Cultures ◽  
Comparative Investigation ◽  
In Vitro Cultures ◽  
Liquid Nutrient Medium ◽  
Whole Plants ◽  
Plant Seedlings

Abstract Unorganized callus and leaf/root-differentiating callus cultures of Pimpinella major have been established in liquid nutrient medium. Their capacity to accumulate rare phenylpropanoids such as epoxy-pseudoisoeugenol tiglate, epoxy-anol tiglate and anol tiglate was compared with that of seedlings and whole plants. The unorganized callus cultures were not able to accumulate any phenylpropanoids. In comparison, the leaf/root-differentiating callus culture promoted the accumulation of epoxy-pseudoisoeugenol tiglate (up to 90 mg/100 g fr.wt.) but not that of anol-derivatives. The accumulated amount of EPT in PMD-SH was comparable with that in plant seedlings.

scholarly journals In vitro metabolism of Tebuconazole, Flurtamone, Fenhexamid, Metalaxyl‐M and Spirodiclofen in Cannabis sativa L. (hemp) callus cultures

2021 ◽  
Author(s):  
Leonie Hillebrands ◽  
Marc Lamshoeft ◽  
Andreas Lagojda ◽  
Andreas Stork ◽  
Oliver Kayser
Keyword(s):  
Cannabis Sativa ◽  
Callus Cultures ◽  
In Vitro Metabolism

Isolation and quantification of antimalarial N -alkylamides from flower-head derived in vitro callus cultures of Spilanthes paniculata

2017 ◽  
Vol 124 (1) ◽  
pp. 99-107 ◽  
Author(s):  
Radhika Rajendran ◽  
Balaji Sitthu Narashimman ◽  
Vishal Trivedi ◽  
Rakhi Chaturvedi
Keyword(s):  
Callus Cultures ◽  
Flower Head

scholarly journals Arabidopsis Cell-free Extract, ACE, a New In Vitro Translation System Derived from Arabidopsis Callus Cultures

2012 ◽  
Vol 53 (3) ◽  
pp. 602-602
Author(s):  
K. Murota ◽  
Y. Hagiwara-Komoda ◽  
K. Komoda ◽  
H. Onouchi ◽  
M. Ishikawa ◽  
...  
Keyword(s):  
Callus Cultures ◽  
In Vitro Translation ◽  
Translation System ◽  
Cell Free Extract ◽  
Arabidopsis Callus

scholarly journals In vitro callus induction and plantlet regeneration of Indian red wood, Soymida febrifuga A. Juss (Roxb.)

2014 ◽  
Vol 8 (24) ◽  
pp. 847-856
Author(s):  
K. Chiruvella Kishore ◽  
Mohammed Arifullah ◽  
Gopal Ghanta Rama ◽  
K. Chiruvella Kishore ◽  
Mohammed Arifullah ◽  
...  
Keyword(s):  
Callus Induction ◽  
Plantlet Regeneration

scholarly journals Assessment of Acid Phosphatase Production by In vitro Cultures of Atropa acuminata

2016 ◽  
Vol 26 (1) ◽  
pp. 15-23
Author(s):  
Saima Khan ◽  
Meenu Katoch ◽  
Sharada Mallubhotla ◽  
Suphla Gupta ◽  
Manju Sambyal ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Specific Activity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Growth Period ◽  
Callus Cultures ◽  
Shoot Cultures ◽  
Crude Enzyme Extract ◽  
Atropa Acuminata

The potential of various culture lines of Atropa acuminata were investigated for resourcing acid phosphatase (ACP) (3.1.3.2). Crude enzyme extract comprised of a mixture of four isoforms, distinguishable by polyacrylamide gel electrophoresis (PAGE) with molecular weight ranging from 39 to 215 kDa. In vitro regenerated proliferative shoots, callus and roots showed higher specific activity (2.49, 3.41, 2.91 U/mg protein, respectively) as compared to in vivo grown plants (0.71 U/mg protein). ACP activity in root cultures increased progressively up to 4.6 U/mg during the entire growth period (2 ? 24 weeks), whereas in case of shoot cultures, the specific activity escalated to 2.49 U/mg at 8 weeks, which then declined subsequently (1.95 U/mg). Similarly, callus cultures initially showed a higher phosphohydrolytic activity (3.41 U/mg protein) until 8 weeks by which period, it decreased with the passage of growth period. The present studies reveal an alternate system for resourcing of ACP from Atropa acuminata.Plant Tissue Cult. & Biotech. 26(1): 15-23, 2016 (June)

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