Substances previously found to be highly toxic when applied to annuals as a spray were also found to be most toxic when added to culture solution. The results by the two methods, however, do not agree as far as the less poisonous chemicals are concerned, certain substances being comparatively more toxic in culture solution than as a spray, and vice versa. These discrepancies can be explained by the fact that the dosage in culture solution was varied by adjusting the concentration, whereas in the spraying test it was varied by altering the volume of spray.The time between treatment and death of the plant generally decreases as the dosage is increased over a limited dosage range, but varies with different chemicals, and appears to be independent of their inherent toxicity.The size of the plant is seriously reduced at dosages that produce no mortality. The final weight, however, was unsatisfactory as a criterion of toxicity since it was extremely variable. The interfering factors affecting the final weight were taken into account by computing the relative growth rate. The curve relating growth rate and dosage is slightly concave upwards when the dosage is plotted on an arithmetical scale and linear when plotted on a logarithmic scale. The position and slope of the line depends on the chemical. The standard error of duplicate tests increases as the growth rate decreases. On the average, complete mortality occurred at a growth rate of − 2.44% per day under the conditions of these experiments, but this is subject to variation due to differences between duplicates, chemicals, and series (plants grown at different times).Analyses of the culture solutions containing chlorates showed that the amount of chlorate taken up by the plant increased with the concentration present in the culture solution. Nevertheless, only a small, relatively constant proportion of the chlorate present was taken up by the plant at all concentrations.
Study on dosage range evaluation opioid analgesic for breakthrough pain in cancer patients: a retrospective study
