Integrated fabrication-conjugation approaches for biomolecular assembly and protein sensing with hybrid microparticle platforms and biofabrication - A focused minireview

OBPs play a crucial role in the recognition of ligands and are involved in the initial steps of semiochemical perception. The diverse expression of OBP genes allows them to participate in different physiological functions in insects. In contrast to classic OBPs with typical olfactory roles in A. lineolatus, the physiological functions of Plus-C OBPs remain largely unknown. In addition, detection of the expression of insect OBP genes by conventional methods is difficult in vitro. Here, we focused on AlinOBP14, a Plus-C OBP from A. lineolatus, and we developed a PNA-GO-based mRNA biosensor to detect the expression of AlinOBP14. The results demonstrated that AlinOBP14 plays dual roles in A. lineolatus. The AlinOBP14 is expressed beneath the epidermis of the vertex and gena in heads of A. lineolatus, and it functions as a carrier for three terpenoids, while AlinOBP14 is also expressed in the peripheral antennal lobe and functions as a carrier for endogenous compounds such as precursors for juvenile hormone (JH) and JHⅢ. Our investigation provides a new method to detect the expression of OBP genes in insects, and the technique will facilitate the use of these genes as potential targets for novel insect behavioral regulation strategies against the pest.

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Nanomolar Protein Sensing with Embedded Receptor Molecules

Journal of the American Chemical Society ◽

10.1021/ja045785d ◽

2005 ◽

Vol 127 (3) ◽

pp. 904-915 ◽

Cited By ~ 110

Author(s):

Reza Zadmard ◽

Thomas Schrader

Keyword(s):

Protein Sensing

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Surface Plasmon Resonance Enhanced Real-Time Photoelectrochemical Protein Sensing by Gold Nanoparticle-Decorated TiO2 Nanowires

Analytical Chemistry ◽

10.1021/ac501406x ◽

2014 ◽

Vol 86 (13) ◽

pp. 6633-6639 ◽

Cited By ~ 70

Author(s):

Peimei Da ◽

Wenjie Li ◽

Xuan Lin ◽

Yongcheng Wang ◽

Jing Tang ◽

...

Keyword(s):

Surface Plasmon Resonance ◽

Real Time ◽

Gold Nanoparticle ◽

Surface Plasmon ◽

Plasmon Resonance ◽

Tio2 Nanowires ◽

Protein Sensing

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Hybrid Material for Protein Sensing Based on Electrosynthesized MIP on a Mannose Terminated Self-Assembled Monolayer

Advanced Functional Materials ◽

10.1002/adfm.201303148 ◽

2013 ◽

Vol 24 (15) ◽

pp. 2233-2239 ◽

Cited By ~ 49

Author(s):

Decha Dechtrirat ◽

Nenad Gajovic-Eichelmann ◽

Frank F. Bier ◽

Frieder W. Scheller

Keyword(s):

Hybrid Material ◽

Self Assembled Monolayer ◽

Protein Sensing ◽

Self Assembled

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ATP compartmentation in plastids and cytosol ofArabidopsis thalianarevealed by fluorescent protein sensing

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1711497115 ◽

2018 ◽

Vol 115 (45) ◽

pp. E10778-E10787 ◽

Cited By ~ 16

Author(s):

Chia Pao Voon ◽

Xiaoqian Guan ◽

Yuzhe Sun ◽

Abira Sahu ◽

May Ngor Chan ◽

...

Keyword(s):

Arabidopsis Thaliana ◽

Fluorescent Protein ◽

Higher Plants ◽

Electron Flow ◽

Cyclic Electron Flow ◽

Linear Electron ◽

Protein Sensing ◽

Atp Production ◽

Respiratory Inhibitors ◽

Sensor Protein

Matching ATP:NADPH provision and consumption in the chloroplast is a prerequisite for efficient photosynthesis. In terms of ATP:NADPH ratio, the amount of ATP generated from the linear electron flow does not meet the demand of the Calvin–Benson–Bassham (CBB) cycle. Several different mechanisms to increase ATP availability have evolved, including cyclic electron flow in higher plants and the direct import of mitochondrial-derived ATP in diatoms. By imaging a fluorescent ATP sensor protein expressed in livingArabidopsis thalianaseedlings, we found that MgATP2−concentrations were lower in the stroma of mature chloroplasts than in the cytosol, and exogenous ATP was able to enter chloroplasts isolated from 4- and 5-day-old seedlings, but not chloroplasts isolated from 10- or 20-day-old photosynthetic tissues. This observation is in line with the previous finding that the expression of chloroplast nucleotide transporters (NTTs) inArabidopsismesophyll is limited to very young seedlings. Employing a combination of photosynthetic and respiratory inhibitors with compartment-specific imaging of ATP, we corroborate the dependency of stromal ATP production on mitochondrial dissipation of photosynthetic reductant. Our data suggest that, during illumination, the provision and consumption of ATP:NADPH in chloroplasts can be balanced by exporting excess reductants rather than importing ATP from the cytosol.

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