Establishment of a high-frequency plant regeneration system from rhizome-derived embryogenic cell-suspension cultures of Curcuma longa L.

2019 ◽  
Vol 13 (2) ◽  
pp. 123-129 ◽  
Author(s):  
Eun Yee Jie ◽  
Myung Suk Ahn ◽  
Jiyoung Lee ◽  
Ye In Cheon ◽  
Cha Young Kim ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
High Frequency ◽  
Curcuma Longa ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Regeneration System ◽  
Embryogenic Cell Suspension ◽  
Embryogenic Cell

High-frequency plant regeneration from embryogenic cell suspension cultures of Gynura procumbens

2018 ◽  
Vol 13 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Eun Yee Jie ◽  
Nornita Sham Atong ◽  
Woo Seok Ahn ◽  
Myung Suk Ahn ◽  
Byung Hwan Min ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
High Frequency ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Embryogenic Cell Suspension ◽  
Embryogenic Cell

scholarly journals Efficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hyun-A Woo ◽  
Seong Sub Ku ◽  
Eun Yee Jie ◽  
HyeRan Kim ◽  
Hyun-Soon Kim ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
Embryogenic Callus ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Regeneration System ◽  
Embryogenic Cell ◽  
Embryogenic Callus Formation

AbstractTo establish an efficient plant regeneration system from cell suspension cultures of Euonymus alatus, embryogenic callus formation from immature embryos was investigated. The highest frequency of embryogenic callus formation reached 50% when the immature zygotic embryos were incubated on Murashige and Skoog (MS) medium supplemented with 1 mg/L 2,4-dichlorophenoxy acetic acid (2,4-D). At higher concentrations of 2,4-D (over 2 mg/L), the frequency of embryogenic callus formation declined significantly. The total number of somatic embryos development was highest with the 3% (w/v) sucrose treatment, which was found to be the optimal concentration for somatic embryo formation. Activated charcoal (AC) and 6-benzyladenine (BA) significantly increased the frequency of plantlet conversion from somatic embryos, but gibberellic acid (GA3) had a negative effect on plantlet conversion and subsequent development from somatic embryos. Even though the cell suspension cultures were maintained for more than 1 year, cell aggregates from embryogenic cell suspension cultures were successfully converted into normal somatic embryos with two cotyledons. To our knowledge, this is the first successful report of a plant regeneration system of E. alatus via somatic embryogenesis. Thus, the embryogenic cell line and plant regeneration system established in this study can be applied to mass proliferation and production of pharmaceutical metabolite in E. alatus.

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