Relations Between Moso Bamboo Surface Properties Pretreated by Kraft Cooking and Dilute Acid with Enzymatic Digestibility

2017 ◽  
Vol 183 (4) ◽  
pp. 1526-1538 ◽  
Author(s):  
Juan He ◽  
Caoxing Huang ◽  
Chenhuan Lai ◽  
Chen Huang ◽  
Qiang Yong
Keyword(s):  
Surface Properties ◽  
Moso Bamboo ◽  
Dilute Acid ◽  
Enzymatic Digestibility ◽  
Kraft Cooking

scholarly journals Understanding the effects of different residual lignin fractions in acid-pretreated bamboo residues on its enzymatic digestibility

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Wenqian Lin ◽  
Jinlai Yang ◽  
Yayue Zheng ◽  
Caoxing Huang ◽  
Qiang Yong
Keyword(s):  
Enzymatic Hydrolysis ◽  
Negative Impact ◽  
Dilute Acid Pretreatment ◽  
Residual Lignin ◽  
Dilute Acid ◽  
Enzymatic Digestibility ◽  
Acid Pretreatment ◽  
Organic Reagents ◽  
Milled Wood Lignin ◽  
Affinity Constants

Abstract Background During the dilute acid pretreatment process, the resulting pseudo-lignin and lignin droplets deposited on the surface of lignocellulose and inhibit the enzymatic digestibility of cellulose in lignocellulose. However, how these lignins interact with cellulase enzymes and then affect enzymatic hydrolysis is still unknown. In this work, different fractions of surface lignin (SL) obtained from dilute acid-pretreated bamboo residues (DAP-BR) were extracted by various organic reagents and the residual lignin in extracted DAP-BR was obtained by the milled wood lignin (MWL) method. All of the lignin fractions obtained from DAP-BR were used to investigate the mechanism for interaction between lignin and cellulase using surface plasmon resonance (SPR) technology to understand how they affect enzymatic hydrolysis Results The results showed that removing surface lignin significantly decreased the yield for enzymatic hydrolysis DAP-BR from 36.5% to 18.6%. The addition of MWL samples to Avicel inhibited its enzymatic hydrolysis, while different SL samples showed slight increases in enzymatic digestibility. Due to the higher molecular weight and hydrophobicity of MWL samples versus SL samples, a stronger affinity for MWL (KD = 6.8–24.7 nM) was found versus that of SL (KD = 39.4–52.6 nM) by SPR analysis. The affinity constants of all tested lignins exhibited good correlations (r > 0.6) with the effects on enzymatic digestibility of extracted DAP-BR and Avicel. Conclusions This work revealed that the surface lignin on DAP-BR is necessary for maintaining enzyme digestibility levels, and its removal has a negative impact on substrate digestibility.

scholarly journals Investigation of interaction between cellulase and residual lignin fractions in acid-pretreated bamboo residues and their effect on enzymatic digestibility

2021 ◽  
Author(s):  
Wenqian Lin ◽  
Jinlai Yang ◽  
Yayue Zheng ◽  
Caoxing Huang ◽  
Qiang Yong
Keyword(s):  
Enzymatic Hydrolysis ◽  
Negative Impact ◽  
Interaction Mechanism ◽  
Dilute Acid Pretreatment ◽  
Affinity Constant ◽  
Residual Lignin ◽  
Dilute Acid ◽  
Enzymatic Digestibility ◽  
Acid Pretreatment ◽  
Hydrolysis Of

Abstract Background: During dilute acid pretreatment, pseudo lignin and lignin form droplets which deposit on the surface of lignocellulose, and further inhibit its enzymatic hydrolysis. However, how this lignin interacts with cellulase enzymes and then affects enzymatic hydrolysis is still unknown. In this work, different fractions of surface lignin (SL) obtained from dilute acid pretreated bamboo residues (DAP-BR) were extracted by various organic reagents and the residual lignin in extracted DAP-BR was obtained by milled wood lignin (MWL) method. All the obtained lignin fractions from DAP-BR were used to investigate the interaction mechanism between lignin and cellulase using surface plasmon resonance (SPR) technology in order to understand how they affect enzymatic hydrolysisResults: Results showed that removing surface lignin significantly decrease the enzymatic hydrolysis of DAP-BR from 36.5% to 18.6%. The addition of MWL samples to Avicel decreased enzymatic hydrolysis of Avicel, while different SL samples showed a slight increase to its enzymatic digestibility. Due to the higher molecular weight and hydrophobicity of MWL samples versus the SL samples, stronger affinity for MWL (KD = 6.8-24.7 nM) was found versus that of SL (KD = 39.4-52.6 nM) by SPR analysis. The affinity constant of all tested lignin had good correlations (R2>0.6) with their effects on enzymatic digestibility of extracted DAP-BR and Avicel.Conclusions: This work reveals that the surface lignin on DAP-BR is necessary towards maintaining enzyme digestibility levels, and its removal has a negative impact on the substrate’s digestibility.

Effects of Alkali Pretreatment on Surface Properties and Green Color Conservation of Moso Bamboo (Phyllostachys pubescens Mazel)

Holzforschung ◽  
2000 ◽  
Vol 54 (5) ◽  
pp. 487-491 ◽  
Author(s):  
Shang-Tzen Chang ◽  
Ting-Feng Yeh
Keyword(s):  
Surface Properties ◽  
Moso Bamboo ◽  
Surface Wettability ◽  
Color Appearance ◽  
Phyllostachys Pubescens ◽  
Type B ◽  
Alkali Pretreatment ◽  
Green Color ◽  
Copper Arsenate ◽  
Better Than

SummaryStanding bamboo culms have an attractive greenish skin. Once harvested, however, they turn yellow and the color starts to decay. These adverse properties limit their applications and degrade their performance. Chromated copper arsenate (CCA) based preservatives have been reported to be good protectors for preserving the green color of bamboo culms. An alkali pretreatment was required before applying CCA. However, the mechanism of alkali pretreatment on color conservation is not fully understood. In this study, morphology, wettability, chemical and pigment changes of moso bamboo (Phyllostachys pubescensMazel) were studied after treating with four alkali reagents. The results showed that 2% KOH effectively removed the waxes and capes of silica cells on moso bamboo surface and also increased the surface wettability much better than treatments with 4% K2CO3, 10% K2CO3, 4% Na2CO3, or 4% NaHCO3. The KOH pretreatment also results in a better penetration and reaction with the green color protector (5% Boliden K-33, type-B CCA), and subsequently preserved the green color appearance of moso bamboo.

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