Scale-Up of Duddingtonia flagrans Chlamydospores Production from Laboratory to Pilot-Scale Using a Solid-State Fermentation System

Resistance structures such as chlamydospores produced by the fungus Duddingtonia flagrans allow the reduction of infectious larvae from gastrointestinal nematodes. The objective of this research was to study the effect of carbon and nitrogen sources on the production of chlamydospores of the fungus in a solid state fermentation system (SSF). Twelve substances were studied using a statistical strategy, evaluating their effect on the production of Chlamydospores Finally, using an optimization strategy, the modifications of the substances that favor the production of chlamydospores were defined, and the effect of these on the predatory capacity of the fungus was evaluated. Optimal conditions were the variability of 0.25% w / w ammonium sulfate and 0.56% w / w sodium acetate in broken rice. The maximum concentration reached under this condition was 2.27x107 chlamydospores g of dry substrate-1, with a productivity of 1.62x106 chlamydospores g of dry substrate-1 day-1.

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Erratum to: Optimization of Xylanase Production by Filamentous Fungi in Solid-State Fermentation and Scale-up to Horizontal Tube Bioreactor

Applied Biochemistry and Biotechnology ◽

10.1007/s12010-015-1534-1 ◽

2015 ◽

Vol 176 (1) ◽

pp. 317-319 ◽

Cited By ~ 1

Author(s):

N. Pérez-Rodríguez ◽

F. Oliveira ◽

B. Pérez-Bibbins ◽

I. Belo ◽

A. Torrado Agrasar ◽

...

Keyword(s):

Solid State ◽

Filamentous Fungi ◽

Solid State Fermentation ◽

Scale Up ◽

Horizontal Tube ◽

Xylanase Production

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Solid-state fermentation of soybean residues for bioflocculant production in a pilot-scale bioreactor system

Water Science & Technology ◽

10.2166/wst.2014.329 ◽

2014 ◽

Vol 70 (6) ◽

pp. 1032-1039 ◽

Cited By ~ 3

Author(s):

Zufarzaana Zulkeflee ◽

Antoni Sánchez

Keyword(s):

Solid State ◽

Solid State Fermentation ◽

Microbial Growth ◽

Future Prospect ◽

Pilot Scale ◽

Scale Level ◽

Bioactive Materials ◽

Current Dependence ◽

Death Phase ◽

Bioreactor System

An innovative approach using soybean residues for the production of bioflocculants through solid-state fermentation was carried out in 4.5 L near-to-adiabatic bioreactors at pilot-scale level. An added inoculum of the strain Bacillus subtilis UPMB13 was tested in comparison with control reactors without any inoculation after the thermophilic phase of the fermentation. The flocculating performances of the extracted bioflocculants were tested on kaolin suspensions, and crude bioflocculants were obtained from 20 g of fermented substrate through ethanol precipitation. The production of bioflocculants was observed to be higher during the death phase of microbial growth. The bioflocculants were observed to be granular in nature and consisted of hydroxyl, carboxyl and methoxyl groups that aid in their flocculating performance. The results show the vast potential of the idea of using wastes to produce bioactive materials that can replace the current dependence on chemicals, for future prospect in water treatment applications.

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