scholarly journals Chlamydospores production enhancement of Duddingtonia flagrans in a solid-state fermentation system

DYNA ◽  
2021 ◽  
Vol 88 (216) ◽  
pp. 152-159
Author(s):  
Paula Daniela Cuadrado Osorio ◽  
Carlos Rafael Castillo Saldarriaga ◽  
Jaime Andres Cubide Cardenas ◽  
Martha Isabel Gómez Alvarez ◽  
Eddy Johana Bautista Bautista

Resistance structures such as chlamydospores produced by the fungus Duddingtonia flagrans allow the reduction of infectious larvae from gastrointestinal nematodes. The objective of this research was to study the effect of carbon and nitrogen sources on the production of chlamydospores of the fungus in a solid state fermentation system (SSF). Twelve substances were studied using a statistical strategy, evaluating their effect on the production of Chlamydospores Finally, using an optimization strategy, the modifications of the substances that favor the production of chlamydospores were defined, and the effect of these on the predatory capacity of the fungus was evaluated. Optimal conditions were the variability of 0.25% w / w ammonium sulfate and 0.56% w / w sodium acetate in broken rice. The maximum concentration reached under this condition was 2.27x107 chlamydospores g of dry substrate-1, with a productivity of 1.62x106 chlamydospores g of dry substrate-1 day-1.

2020 ◽  
Vol 49 (1) ◽  
pp. 135-140
Author(s):  
Roheena Abdullah ◽  
Maria Hanif ◽  
Afshan Kaleem ◽  
Mehwish Iqtedar ◽  
Kinza Nisar ◽  
...  

The experiment was conducted to isolate and screen fungal strain and optimization of solid-state fermentation conditions for enhanced production of β-glucosidase. Different fungal cultures were isolated and screened for β-glucosidase production. The physicochemical and nutritional parameters were optimized for enhanced production of β-glucosidase from higher producer. Among all the isolates the isolate which exhibited highest β-glucosidase potential was identified and assigned the code as Aspergillus niger MBT-2. The optimum β-glucosidase production was obtained in M5 medium containing wheat bran after 72 hrs of incubation at 40°C, pH 6 and 20 ml of moisture contents. In addition to this 2% fructose and 2% yeast extract proved to be best carbon and nitrogen sources, respectively and gave maximal enzyme productivity. The exploitation of agricultural by products as a substrate reduced the production cost of enzyme and makes the process economical. The Aspergillus niger MBT-2 has promising potential of bioconversion of low-cost material into valuable product like β-glucosidase.


2020 ◽  
Vol 192 (3) ◽  
pp. 1044-1059
Author(s):  
Paula Daniela Cuadrado Osorio ◽  
Carlos Rafael Castillo-Saldarriaga ◽  
Martha Isabel Gómez Alvárez ◽  
Eddy J Bautista

2018 ◽  
Vol 47 (3) ◽  
pp. 473-478 ◽  
Author(s):  
Roheena Abdullah ◽  
Iqra Farooq ◽  
Afshan Kaleem ◽  
Mehwish Iqtedar ◽  
Tehreema Iftikhar

Different fungal strains were isolated from the local soil, fruits and vegetables on the basis of pectin hydrolysis. All the isolated strains were identified through microscopic studies and screened for pectinase production using solid state fermentation. The fungal strain identified as Aspergillus niger IBT-7 showed the highest pectinase production. The selected strain was further subjected to optimization through different physical and nutritional parameters to enhance the production of pectinase. Amongst seven different media tested M1 containing rice bran, moistened with Czapek’s nutrient medium showed the highest pectinase production. During optimization maximum pectinase production was achieved after 72 hrs of incubation at 30 ml of moisture content, pH 5.0 and 30°C. Xylose (1.5%) and yeast extract (1%) proved to be best supplemented carbon and nitrogen sources, respectively which gave the highest pectinase production (39.1 U/ml/min).


2018 ◽  
Vol 11 (1) ◽  
Author(s):  
Changzheng Wu ◽  
Feng Zhang ◽  
Lijun Li ◽  
Zhedong Jiang ◽  
Hui Ni ◽  
...  

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