scholarly journals Formation of interspecies fusants of Agaricus bisporus and Agaricus bitorquis mushroom by protoplast fusion

2007 ◽  
Vol 47 (4) ◽  
pp. 369-372 ◽  
Author(s):  
Rana Inder Singh ◽  
Kanojiya Aarti ◽  
Sandhu Sardul Singh
Keyword(s):  
Protoplast Fusion ◽  
Agaricus Bisporus ◽  
Agaricus Bitorquis

Homology of Agaricus mitochondrial plasmids with mitochondrial DNA

Genome ◽  
1988 ◽  
Vol 30 (5) ◽  
pp. 710-716 ◽  
Author(s):  
Robert J. Meyer ◽  
William A. Hintz ◽  
Madan Mohan ◽  
Mary Robison ◽  
James B. Anderson ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Mitochondrial Genome ◽  
Agaricus Bisporus ◽  
Restriction Maps ◽  
Linear Plasmids ◽  
Homologous Sequences ◽  
Hybridization Probes ◽  
Extrachromosomal Element ◽  
Agaricus Bitorquis ◽  
Bacterial Plasmid

A strain of Agaricus bitorquis (Ag4) contains two linear plasmids, pEM and pMPJ. Restriction maps for these two plasmids were determined. Similar plasmid-like DNAs were found to be present in some other strains of A. bitorquis but none were found in strains of the commercial mushroom Agaricus bisporus. The three internal EcoRI fragments of pEM were cloned into a bacterial plasmid and were subsequently used as hybridization probes. The cloned portion of pEM has homology with sequences present in the mitochondrial genome of strains of A. bisporus and A. bitorquis, even those without pEM or any obvious extrachromosomal element. In addition, there are pEM homologous sequences within the mitochondrial genome of the source strain Ag4, which contain pEM and pMPJ as extrachromosomal elements.Key words: Agaricus, plasmid, mitochondrial DNA.

Properties of Isolated Mitochondria of Agaricus Bisporus: Their Relationship to Dormancy and the Germination of Spores

1973 ◽  
Vol 31 ◽  
pp. 458-459
Author(s):  
K. S. McCarty ◽  
R. F. Weave ◽  
L. Kemper ◽  
F. S. Vogel
Keyword(s):  
Mitochondrial Dna ◽  
Ethidium Bromide ◽  
Microscopic Examination ◽  
Agaricus Bisporus ◽  
Osmotic Shock ◽  
Electron Microscopic Examination ◽  
Electron Microscopic ◽  
Isolated Mitochondria ◽  
Dna Strands ◽  
Cytoplasmic Ribosomes

During the prodromal stages of sporulation in the Basidiomycete, Agaricus bisporus, mitochondria accumulate in the basidial cells, zygotes, in the gill tissues prior to entry of these mitochondria, together with two haploid nuclei and cytoplasmic ribosomes, into the exospores. The mitochondria contain prominent loci of DNA [Fig. 1]. A modified Kleinschmidt spread technique1 has been used to evaluate the DNA strands from purified whole mitochondria released by osmotic shock, mitochondrial DNA purified on CsCl gradients [density = 1.698 gms/cc], and DNA purified on ethidium bromide CsCl gradients. The DNA appeared as linear strands up to 25 u in length and circular forms 2.2-5.2 u in circumference. In specimens prepared by osmotic shock, many strands of DNA are apparently attached to membrane fragments [Fig. 2]. When mitochondria were ruptured in hypotonic sucrose and then fixed in glutaraldehyde, the ribosomes were released for electron microscopic examination.

Artificial Hybridization of Ganoderma lucidum and G. tsugae Murrill by Protoplast Fusion for Sustainability

2005 ◽  
Vol 7 (1-2) ◽  
pp. 263-280 ◽  
Author(s):  
Siu Wai Chiu ◽  
Vivien Wing Yan Luk ◽  
Stephen Yu ◽  
Peggy Lee ◽  
Natalie Wai ◽  
...  
Keyword(s):  
Protoplast Fusion ◽  
Ganoderma Lucidum ◽  
Artificial Hybridization
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