Bone marrow-derived cells are recruited by the melanoma tumor with endothelial cells contributing to tumor vasculature

2016 ◽  
Vol 19 (1) ◽  
pp. 125-133 ◽  
Author(s):  
R. Bonfim-Silva ◽  
L. E. B. Souza ◽  
F. U. F. Melo ◽  
V. C. Oliveira ◽  
D. A. R. Magalhães ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Endothelial Cells ◽  
Tumor Vasculature ◽  
Melanoma Tumor ◽  
Bone Marrow Derived Cells

Abstract 296: Bone Marrow Derived Cells Contribute to Cell Turnover in Ageing Murine Hearts

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Helge Möllmann ◽  
Holger M Nef ◽  
Christian Troidl ◽  
Sandra Voss ◽  
Mathias Heil ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Endothelial Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Cell Sorting ◽  
Physiological Role ◽  
Muscle Cells ◽  
Left Ventricular ◽  
Cell Turnover ◽  
Bone Marrow Derived Cells

Background: The paradigm that the heart is a terminally differentiated organ has recently been challenged since some studies reported the ability of bone marrow (BM) derived cells to transdifferentiate into cardiomyocytes after myocardial damage. However, the physiological role of bone marrow derived cells during the lifespan of an undamaged heart is widely unknown. We therefore examined the quantity and phenotype of bone marrow derived cells in aged murine hearts. Methods: 12-week-old mice (n=20) were sublethally irradiated and BM from enhanced green fluorescent-transgenic (eGFP) littermates was transplanted. After 1 month 5 mice were sacrificed and served as control. The remaining mice were sacrificed after 18.2±1.1 months. Immunohistochemistry was performed in 10 hearts using titin antibodies to identify cardiomyocytes, vimentin for fibroblasts, sMemb for myofibroblasts, α-smooth muscle actin for smooth muscle cells, F4/80 for macrophages, BS-1 and CD31 for endothelial cells. Additionally, anti-eGFP immunostaining was used to exclude autofluorescence. Sections were analyzed using fluorescence and confocal laser microscopy. The remaining 5 hearts were digested with collagenase and cell sorting was performed for a quantification of BM-derived cells in relation to eGFP negative cells. Results: BM transplantation was successful as FACS analysis showed 92±5% eGFP expressing leukocytes after 1 month and 78±6% after 18 months. In the juvenile hearts only few eGFP-positive cells were detected (<1 cell/mm2). Numerous eGFP-positive cells were found in left ventricular sections in the old hearts. Histological quantification revealed 9.3±3.3 cells/mm2 to be derived from BM cells. Most of these cells were fibroblasts and myofibroblasts. In addition, numerous endothelial cells and smooth muscle cells contributing to neoangiogenesis were detected. Few eGFP-positive cardiomyocytes could be identified. The cell sorting of eGFP-positive cells documented 4.8±1.9% of all cardiac cells to be derived of BM cells. Conclusion: The present study demonstrates for the first time a substantial recruitment and accumulation of BM derived cells in the ageing myocardium suggesting their contribution in cell turnover of the heart during the lifespan of mice.

Abstract LB-304: Bone marrow-derived endothelial cells migrate to tumor sites and contribute to functional tumor vasculature

2011 ◽  
Author(s):  
Aparecida M. Fontes ◽  
Ricardo Bonfim-Silva ◽  
Lucas Eduardo Botelho Souza ◽  
Fernanda Ursoli Ferreira Melo ◽  
Viviane Cassia Oliveira ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Endothelial Cells ◽  
Tumor Vasculature

scholarly journals The Contribution of Bone Marrow–Derived Cells to the Tumor Vasculature in Neuroblastoma Is Matrix Metalloproteinase-9 Dependent

2005 ◽  
Vol 65 (8) ◽  
pp. 3200-3208 ◽  
Author(s):  
Sonata Jodele ◽  
Christophe F. Chantrain ◽  
Laurence Blavier ◽  
Carolyn Lutzko ◽  
Gay M. Crooks ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Matrix Metalloproteinase ◽  
Tumor Vasculature ◽  
Matrix Metalloproteinase 9 ◽  
Bone Marrow Derived Cells ◽  
Metalloproteinase 9

A role for bone marrow–derived cells in the vasculature of noninjured CNS

Blood ◽  
2005 ◽  
Vol 105 (6) ◽  
pp. 2400-2402 ◽  
Author(s):  
Francesco Galimi ◽  
Robert G. Summers ◽  
Henriette van Praag ◽  
Inder M. Verma ◽  
Fred H. Gage
Keyword(s):  
Bone Marrow ◽  
Endothelial Cells ◽  
Fluorescent Protein ◽  
Hematopoietic Cells ◽  
Active Role ◽  
Large Numbers ◽  
The Central Nervous System ◽  
Bone Marrow Derived Cells ◽  
Vascular Structures ◽  
Green Fluorescent

AbstractThe contribution of hematopoietic cells to the formation of blood vessels is currently the focus of intense scrutiny. Bone marrow–derived endothelial progenitor cells are thought to generate endothelial cells in many tissues, including myocardium, muscle, and certain tumors. In the central nervous system (CNS), however, the possible role of bone marrow–derived angiocompetent cells remains unclear. Here we have investigated the long-term involvement of bone marrow–derived cells in the maintenance of endothelial structures in the brain, spinal cord, and retina. Using hematopoietic chimeras stably expressing green fluorescent protein (GFP) in bone marrow–derived tissues, we found large numbers of hematopoietic cells closely associated with vessels in the CNS. None of these cells, however, showed an endothelial phenotype. They were positive for monocytic and microglial surface markers and demonstrated active phagocytosis of neighboring endothelial elements. Bone marrow–derived, vasculature-associated cells in the noninjured adult CNS are distinct from endothelial cells, but play an active role in vascular structures.

scholarly journals Bone marrow-derived cells serve as proangiogenic macrophages but not endothelial cells in wound healing

Blood ◽  
2011 ◽  
Vol 117 (19) ◽  
pp. 5264-5272 ◽  
Author(s):  
Yuji Okuno ◽  
Ayako Nakamura-Ishizu ◽  
Kazuo Kishi ◽  
Toshio Suda ◽  
Yoshiaki Kubota
Keyword(s):  
Bone Marrow ◽  
Wound Healing ◽  
Endothelial Cells ◽  
Cancer Progression ◽  
Fluorescent Protein ◽  
Healing Process ◽  
Decubitus Ulcer ◽  
Endothelial Differentiation ◽  
Bone Marrow Derived Cells ◽  
Green Fluorescent

Abstract Bone marrow-derived cells (BMDCs) contribute to postnatal vascular growth by differentiating into endothelial cells or secreting angiogenic factors. However, the extent of their endothelial differentiation highly varies according to the angiogenic models used. Wound healing is an intricate process in which the skin repairs itself after injury. As a process also observed in cancer progression, neoangiogenesis into wound tissues is profoundly involved in this healing process, suggesting the contribution of BMDCs. However, the extent of the differentiation of BMDCs to endothelial cells in wound healing is unclear. In this study, using the green fluorescent protein-bone marrow chim-eric experiment and high resolution confocal microscopy at a single cell level, we observed no endothelial differentiation of BMDCs in 2 acute wound healing models (dorsal excisional wound and ear punch) and a chronic wound healing model (decubitus ulcer). Instead, a major proportion of BMDCs were macrophages. Indeed, colony-stimulating factor 1 (CSF-1) inhibition depleted approximately 80% of the BMDCs at the wound healing site. CSF-1–mutant (CSF-1op/op) mice showed significantly reduced neoangiogenesis into the wound site, supporting the substantial role of BMDCs as macrophages. Our data show that the proangiogenic effects of macrophages, but not the endothelial differentiation, are the major contribution of BMDCs in wound healing.

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