Morphological structure of propagules and electrophoretic karyotype analysis of false smut Villosiclava virens in rice

2012 ◽  
Vol 50 (2) ◽  
pp. 263-269 ◽  
Author(s):  
Rongtao Fu ◽  
Lei Ding ◽  
Jun Zhu ◽  
Ping Li ◽  
Ai-ping Zheng
Keyword(s):  
Karyotype Analysis ◽  
Morphological Structure ◽  
Electrophoretic Karyotype ◽  
Villosiclava Virens ◽  
False Smut

Loop-mediated Isothermal Amplification for Rapid Detection of Mating Types of Villosiclava virens

Plant Disease ◽  
2021 ◽  
Author(s):  
Xiayan Pan ◽  
Xiao Wang ◽  
Junjie Yu ◽  
Mina Yu ◽  
Huijuan Cao ◽  
...  
Keyword(s):  
Mating Type ◽  
Genomic Dna ◽  
High Efficiency ◽  
Lamp Assay ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Villosiclava Virens ◽  
False Smut ◽  
Mating Type Genes ◽  
Polymerase Chain

Rice false smut (RFS), caused by Villosiclava virens, is an important fungal disease in panicle of rice. V. virens is a heterothallic ascomycete that controlled by two opposite idiomorphs, MAT1-1 and MAT1-2. Previous study showed sexual reproduction of V. virens plays an important role in the epidemic of RFS. In this study, we have developed a loop-mediated isothermal amplification (LAMP) assay to detect mating type of V. virens easily and rapidly by using specific primers designed on the mating type genes MAT1-1-2 and MAT1-2-1, respectively. The LAMP assay required only a water/dry bath and could recognize the mating type of V. virens in just 45 min. The LAMP assay was so sensitive that could detect small amounts of V. virens genomic DNA (as low as 2.0 pg of MAT1-1, and 200.0 pg of MAT1-2), which was 10-fold more sensitive than polymerase chain reaction (PCR). In addition, the application of mating type using LAMP assay was demonstrated feasibly by assessing the genomic DNA of V. virens isolated from rice fields. The high efficiency and specificity of this LAMP assay suggested it can be used as a rapid testing tool in mating type recognition of V. virens isolates in the field.

scholarly journals Characterization of mating-type loci in rice false smut fungus Villosiclava virens

2015 ◽  
Vol 362 (9) ◽  
Author(s):  
Jun-jie Yu ◽  
Wen-xian Sun ◽  
Mi-na Yu ◽  
Xiao-le Yin ◽  
Xiang-kun Meng ◽  
...  
Keyword(s):  
Mating Type ◽  
Smut Fungus ◽  
Rice False Smut ◽  
Villosiclava Virens ◽  
False Smut

Electrophoretic karyotype analysis of Botrytis cinerea

1993 ◽  
Vol 99 (S3) ◽  
pp. 119-128 ◽  
Author(s):  
J. A. L. Kan ◽  
A. Goverse ◽  
C. J. B. Vlugt-Bergmans
Keyword(s):  
Botrytis Cinerea ◽  
Karyotype Analysis ◽  
Electrophoretic Karyotype

scholarly journals Survey and examination of the potential alternative hosts of Villosiclava virens, the pathogen of rice false smut, in China

2015 ◽  
Vol 14 (7) ◽  
pp. 1332-1337 ◽  
Author(s):  
Qi-de DENG ◽  
Ming-li YONG ◽  
Dan-yang LI ◽  
Chao-hui LAI ◽  
Hong-ming CHEN ◽  
...  
Keyword(s):  
Alternative Hosts ◽  
Rice False Smut ◽  
Villosiclava Virens ◽  
False Smut ◽  
Potential Alternative

New Ustilaginoidins from Rice False Smut Balls Caused by Villosiclava virens and Their Phytotoxic and Cytotoxic Activities

2017 ◽  
Vol 65 (25) ◽  
pp. 5151-5160 ◽  
Author(s):  
Weibo Sun ◽  
Ali Wang ◽  
Dan Xu ◽  
Weixuan Wang ◽  
Jiajia Meng ◽  
...  
Keyword(s):  
Cytotoxic Activities ◽  
Rice False Smut ◽  
Villosiclava Virens ◽  
False Smut

New nitrogen-containing metabolites from cultures of rice false smut pathogen Villosiclava virens

2019 ◽  
Vol 35 (2) ◽  
pp. 272-281
Author(s):  
Ali Wang ◽  
Peng Li ◽  
Peipei Han ◽  
Gan Gu ◽  
Tijiang Shan ◽  
...  
Keyword(s):  
Rice False Smut ◽  
Villosiclava Virens ◽  
False Smut

scholarly journals A BAC based physical map and genome survey of the rice false smut fungus Villosiclava virens

BMC Genomics ◽  
2013 ◽  
Vol 14 (1) ◽  
pp. 883 ◽  
Author(s):  
Xiaoming Wang ◽  
Qingli Liu ◽  
Hao Wang ◽  
Chao-Xi Luo ◽  
Gejiao Wang ◽  
...  
Keyword(s):  
Physical Map ◽  
Smut Fungus ◽  
Rice False Smut ◽  
Genome Survey ◽  
Villosiclava Virens ◽  
False Smut
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