Production of an endoinulinase from Aspergillus niger AUMC 9375, by solid state fermentation of agricultural wastes, with purification and characterization of the free and immobilized enzyme

2014 ◽  
Vol 52 (5) ◽  
pp. 389-398 ◽  
Author(s):  
Manal M. Housseiny
BioResources ◽  
2014 ◽  
Vol 9 (4) ◽  
Author(s):  
Valesca Weingartner Montibeller ◽  
Luciana Porto de Souza Vandenberghe ◽  
Antonella Amore ◽  
Carlos Ricardo Soccol ◽  
Leila Birolo ◽  
...  

2018 ◽  
Vol 81 (1) ◽  
Author(s):  
Halifah Pagarra ◽  
Roshanida A. Rahman ◽  
Nur Izyan Wan Azelee ◽  
Rosli Md Illias

Polygalacturonases represent an important member of pectinases group of enzymes with immense industrial applications. The activity of exo-polygalacturonase produced by Aspergillus niger was studied in solid state fermentation (SSF) using Nephrolepis biserrata leaves as substrate. Central composite design (CCD) was used to optimize four significant variables resulted from the screening process that has been initially analyzed for the production of exo-polygalacturonase which are incubation time, temperature, concentration of pectin and moisture content. The optimum exo-polygalacturonase production obtained was 54.64 U/g at 120 hours of incubation time, temperature at 340C, 5.0 g/L of pectin concentration and 75.26% of moisture content. For partial characterization of exo-polygalacturonase, the optimum temperature and pH were obtained at 50°C and pH 4.0, respectively. SDS-PAGE analysis showed that molecular weight of exo-polygalacturonase were 35 and 71 kDa. This study has revealed a significant production of exo-polygalacturonase by A. niger under SSF using cheap and easily available substrate and thus could found immense potential application in industrial sectors and biotechnology


BioResources ◽  
2013 ◽  
Vol 8 (4) ◽  
Author(s):  
Jorge Álvarez-Cervantes ◽  
Edna Maria Hernández-Domínguez ◽  
Ainhoa Arana-Cuenca ◽  
Gerardo Díaz-Godínez ◽  
Yuridia Mercado-Flores

2020 ◽  
Vol 142 ◽  
pp. 104106 ◽  
Author(s):  
Fatma Kaaniche ◽  
Abdelaaty Hamed ◽  
Lobna Elleuch ◽  
Ahlem Chakchouk-Mtibaa ◽  
Slim Smaoui ◽  
...  

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