Characterization of Hemicellulolytic Enzymes Produced by Aspergillus niger NRRL 328 under Solid State Fermentation on Soybean Husks

Polygalacturonases represent an important member of pectinases group of enzymes with immense industrial applications. The activity of exo-polygalacturonase produced by Aspergillus niger was studied in solid state fermentation (SSF) using Nephrolepis biserrata leaves as substrate. Central composite design (CCD) was used to optimize four significant variables resulted from the screening process that has been initially analyzed for the production of exo-polygalacturonase which are incubation time, temperature, concentration of pectin and moisture content. The optimum exo-polygalacturonase production obtained was 54.64 U/g at 120 hours of incubation time, temperature at 340C, 5.0 g/L of pectin concentration and 75.26% of moisture content. For partial characterization of exo-polygalacturonase, the optimum temperature and pH were obtained at 50°C and pH 4.0, respectively. SDS-PAGE analysis showed that molecular weight of exo-polygalacturonase were 35 and 71 kDa. This study has revealed a significant production of exo-polygalacturonase by A. niger under SSF using cheap and easily available substrate and thus could found immense potential application in industrial sectors and biotechnology

Download Full-text

Statistical optimization of cellulases production by Aspergillus niger HQ-1 in solid-state fermentation and partial enzymatic characterization of cellulases on hydrolyzing chitosan

Annals of Microbiology ◽

10.1007/s13213-011-0300-z ◽

2011 ◽

Vol 62 (2) ◽

pp. 629-645 ◽

Cited By ~ 8

Author(s):

Hui Zhang ◽

Qing Sang ◽

Wenhui Zhang

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Solid State Fermentation ◽

Statistical Optimization ◽

Enzymatic Characterization

Download Full-text

Production of an endoinulinase from Aspergillus niger AUMC 9375, by solid state fermentation of agricultural wastes, with purification and characterization of the free and immobilized enzyme

The Journal of Microbiology ◽

10.1007/s12275-014-3561-y ◽

2014 ◽

Vol 52 (5) ◽

pp. 389-398 ◽

Cited By ~ 13

Author(s):

Manal M. Housseiny

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Solid State Fermentation ◽

Immobilized Enzyme ◽

Agricultural Wastes ◽

Purification And Characterization

Download Full-text

Production and Characterization of Amylases by Aspergillus niger under Solid State Fermentation Using Agro Industrials Products

International Journal of Food Engineering ◽

10.2202/1556-3758.1116 ◽

2006 ◽

Vol 2 (3) ◽

Cited By ~ 9

Author(s):

Michele Rigon Spier ◽

Adenise Lorenci Woiciechowski ◽

Luciana Porto de Souza Vandenberghe ◽

Carlos Ricardo Soccol

Keyword(s):

Solid State ◽

Aspergillus Niger ◽

Solid State Fermentation ◽

Sugar Cane ◽

Maximum Yield ◽

Initial Moisture Content ◽

Cassava Starch ◽

Sugar Cane Bagasse ◽

Production Costs

The use of agro industrials products cassava starch and sugar cane bagasse present a great potential as substrate and support, respectively, showing low production costs for amylases production under solid-state fermentation by Aspergillus niger LPB 28. The effect of various factors was examined and some variables were optimized to ?-amylase and amyloglucosidase production. Inoculum rate in different concentrations (105, 106, 107 and 108 spores/g of cassava starch) were used to inoculate the solid substrate for the SSF. The material was supplemented with nitrogen sources; calcium source and micronutrients solution. The ratios of cassava starch to sugar cane bagasse (1/1; 1.5/1; 1/1.5; 1/2; 2/1; 1/3; 3/1) were tested and the initial moisture content varying 80, 85 and 90%. Cultivation was carried out at temperatures 25, 30 and 35ºC for 48, 60 and 72 h. Study of influence of pH initial in SSF was conduced in pH 4.0, 5.0, 6.0, 7.0 and 8.0. Statistical analysis was carried out using the software STATISTICA 5.1 (StatSoft, Tulsa, OK, USA). A 32 complete factorial design (2 factors, 3 levels and 9 experiments) was used to study the effect of the factors in ?-amylase and amyloglucosidase production. The results showed cassava starch and sugar cane bagasse at the ratios of 2/1 was the best for optimum production of ?-amylase and amyloglucosidase. The maximum yield (?-amylase 1732.95 U/g of cassava starch and amyloglucosidase 2044.94 U/g of cassava starch) was achieved with optimized process parameters such as incubation period (60 hours), moisture level was 90%, inoculation rate (105 spores/g of dried material), pH 4.0 and fermentation temperature (30ºC). The nitrogen source (KNO3) also enhanced the ?-amylase and amyloglucosidase activities. In kinetic characterization of enzymes the Michaelis-Menten constant KM and maximum velocity Vmax (KM 10.84 g/l, Vmax 3.42 g/l) for ?-amylase and KM 11.32 g/l and Vmax 5.18 g/l for amyloglucosidase.

Download Full-text