Characterization of cell death in Escherichia coli mediated by XseA, a large subunit of exonuclease VII

2015 ◽  
Vol 53 (12) ◽  
pp. 820-828 ◽  
Author(s):  
Hyeim Jung ◽  
Junwei Liang ◽  
Yuna Jung ◽  
Dongbin Lim
Keyword(s):  
Escherichia Coli ◽  
Cell Death ◽  
Large Subunit ◽  
Exonuclease Vii

scholarly journals Escherichia coli exonuclease VII. Cloning and sequencing of the gene encoding the large subunit (xseA).

1986 ◽  
Vol 261 (32) ◽  
pp. 14929-14935
Author(s):  
J W Chase ◽  
B A Rabin ◽  
J B Murphy ◽  
K L Stone ◽  
K R Williams
Keyword(s):  
Escherichia Coli ◽  
Large Subunit ◽  
Cloning And Sequencing ◽  
Gene Encoding ◽  
Exonuclease Vii

scholarly journals Characterization of Cell Lysis in Pseudomonas putida Induced upon Expression of Heterologous Killing Genes

1998 ◽  
Vol 64 (12) ◽  
pp. 4904-4911 ◽  
Author(s):  
M. Carmen Ronchel ◽  
Lázaro Molina ◽  
Angela Witte ◽  
Werner Lutbiz ◽  
Søren Molin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cell Death ◽  
Pseudomonas Putida ◽  
Membrane Permeability ◽  
Cell Lysis ◽  
Physiological Effects ◽  
Extracellular Medium ◽  
Biological Containment ◽  
Potential Applications

ABSTRACT Active biological containment systems are based on the controlled expression of killing genes. These systems are of interest for thePseudomonadaceae because of the potential applications of these microbes as bioremediation agents and biopesticides. The physiological effects that lead to cell death upon the induction of expression of two different heterologous killing genes in nonpathogenicPseudomonas putida KT2440 derivatives have been analyzed.P. putida CMC4 and CMC12 carry in their chromosomes a fusion of the PA1-04/03 promoter to the Escherichia coli gef gene and the φX174 lysis gene E, respectively. Expression of the killing genes is controlled by the LacI protein, whose expression is initiated from the XylS-dependent Pm promoter. Under induced conditions, killing of P. putidaCMC12 cells mediated by φX174 lysis protein E was faster than that observed for P. putida CMC4, for which the Gef protein was the killing agent. In both cases, cell death occurred as a result of impaired respiration, altered membrane permeability, and the release of some cytoplasmic contents to the extracellular medium.

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