scholarly journals Evaluation of Flow Rate, pH, Buffering Capacity, Calcium, Total Proteins and Total Antioxidant Capacity Levels of Saliva in Caries Free and Caries Active Children: An In Vivo Study

2010 ◽  
Vol 25 (4) ◽  
pp. 425-428 ◽  
Author(s):  
B. P. Preethi ◽  
Dodawad Reshma ◽  
Pyati Anand
Keyword(s):  
Antioxidant Capacity ◽  
Flow Rate ◽  
Total Antioxidant Capacity ◽  
Buffering Capacity ◽  
In Vivo Study ◽  
Total Proteins ◽  
Ph Buffering ◽  
Total Antioxidant ◽  
Ph Buffering Capacity

Salivary Flow Rate, pH, Buffering Capacity, Total Protein, Oxidative Stress and Antioxidant Capacity in Children with and without Dental Caries

2018 ◽  
Vol 42 (6) ◽  
pp. 445-449 ◽  
Author(s):  
Sudharani A Pyati ◽  
R Naveen Kumar ◽  
Vinod Kumar ◽  
N H Praveen Kumar ◽  
K M Parveen Reddy
Keyword(s):  
Antioxidant Capacity ◽  
Flow Rate ◽  
Total Protein ◽  
Chemical Properties ◽  
Salivary Flow ◽  
Buffering Capacity ◽  
Salivary Flow Rate ◽  
Ph Buffering ◽  
Total Antioxidant ◽  
Ph Buffering Capacity

Objectives: To measure and compare the levels of salivary flow rate, pH, buffering capacity, total protein, malondialdehyde (MDA) and total antioxidant capacity (TAC) between caries active and caries free children and to study the correlation between the DMFS/dfs score and above salivary parameters in caries active children. Study design: 50 caries active (DMFS/dfs ≥ 5) and 50 caries free (DMFS/dfs = 0) children aged between 6 to 12 years were included in the study. From all the children, unstimulated, mid-morning saliva samples were collected and salivary flow rate was calculated. Salivary pH, buffering capacity, total protein, MDA and TAC were measured. Results: The mean levels of salivary flow rate, pH, buffering capacity were significantly decreased (p < 0.05) and total protein, MDA and TAC were significantly increased (p < 0.05) in caries active children when compared to caries free controls. There was a proportionate decrease (p < 0.05) in salivary flow rate, pH and buffering capacity and proportionate increase (p > 0.05) in salivary total protein, MDA and TAC as DMFS/dfs score increased in caries active children. Conclusions: Significant alteration in the levels of salivary flow rate, pH, total proteins, MDA and TAC and their correlation with DMFS/dfs score in caries active children suggest, the levels of these physico-chemical properties of saliva can act as strong indicators of caries status in children.

scholarly journals Evaluation of pH, buffering capacity, viscosity and flow rate levels of saliva in caries-free, minimal caries and nursing caries children: An in vivo study

2014 ◽  
Vol 5 (3) ◽  
pp. 324 ◽  
Author(s):  
Dwitha Animireddy ◽  
VenkataThimma Reddy Bekkem ◽  
Pranitha Vallala ◽  
SunilBabu Kotha ◽  
Swetha Ankireddy ◽  
...  
Keyword(s):  
Flow Rate ◽  
Buffering Capacity ◽  
In Vivo Study ◽  
Ph Buffering ◽  
Ph Buffering Capacity

scholarly journals Method validation to assess in vivo cellular and subcellular changes in buccal mucosa cells and saliva following CBCT examinations

2019 ◽  
Vol 48 (6) ◽  
pp. 20180428 ◽  
Author(s):  
Niels Belmans ◽  
Liese Gilles ◽  
Piroska Virag ◽  
Mihaela Hedesiu ◽  
Benjamin Salmon ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Cone Beam Ct ◽  
Immunocytochemical Staining ◽  
Dna Double Strand Breaks ◽  
Age Related ◽  
Dna Dsbs ◽  
Total Antioxidant

Objectives: Cone-beam CT (CBCT) is a medical imaging technique used in dental medicine. However, there are no conclusive data available indicating that exposure to X-ray doses used by CBCT are harmless. We aim, for the first time, to characterize the potential age-dependent cellular and subcellular effects related to exposure to CBCT imaging. Current objective is to describe and validate the protocol for characterization of cellular and subcellular changes after diagnostic CBCT. Methods: Development and validation of a dedicated two-part protocol: 1) assessing DNA double strand breaks (DSBs) in buccal mucosal (BM) cells and 2) oxidative stress measurements in saliva samples. BM cells and saliva samples are collected prior to and 0.5 h after CBCT examination. BM cells are also collected 24 h after CBCT examination. DNA DSBs are monitored in BM cells via immunocytochemical staining for γH2AX and 53BP1. 8-oxo-7,8-dihydro-2’-deoxyguanosine (8-oxo-dG) and total antioxidant capacity are measured in saliva to assess oxidative damage. Results: Validation experiments show that sufficient BM cells are collected (97.1 ± 1.4 %) and that γH2AX/53BP1 foci can be detected before and after CBCT examination. Collection and analysis of saliva samples, either sham exposed or exposed to IR, show that changes in 8-oxo-dG and total antioxidant capacity can be detected in saliva samples after CBCT examination. Conclusion: The DIMITRA Research Group presents a two-part protocol to analyze potential age-related biological differences following CBCT examinations. This protocol was validated for collecting BM cells and saliva and for analyzing these samples for DNA DSBs and oxidative stress markers, respectively.

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