Relative toxicity of two insecticides to Coccinella septempunctata and Hippodamia variegata (Coleoptera: Coccinellidae): Implications for Integrated Management of the aphids, Myzus persicae and Aphis fabae (Hemiptera: Aphididae)

2021 ◽  
Author(s):  
Panagiotis J. Skouras ◽  
Vasilis Demopoulos ◽  
Marina Mprokaki ◽  
Kostas Anagnostelis ◽  
Anastasios I. Darras ◽  
...  
Author(s):  
Тамара Алексеевна Шелабина ◽  
Марина Николаевна Берим

Существенным элементом в комплексе специальных и агротехнических приемов, направленных на ограничение распространения вирусной инфекции, является мониторинг численности, динамики и видового состава тлей, мигрирующих в посадках оригинального картофеля. Двухлетний мониторинг (2016 – 2017 гг.) на опытных полях Гатчинского района Ленинградской области проведен с использованием желтых водных ловушек Мёрике. Изучены количество тлей, сроки их миграции на посадки картофеля, сроки наступления пика численности. Наибольшее влияние на насекомых оказывала среднесуточная температура воздуха. За сезон наблюдений отловлено: в 2016 г. — 481 крылатая особь 31 вида, в 2017 г. — 131 особь 30 видов. Доля возможных переносчиков вирусов картофеля от общего числа зарегистрированных крылатых тлей составила в 2016 г. 14,8 %, в 2017 г. — 32,8 %. Оценка скрытой зараженности вирусной инфекцией (MBK, YBK вирусы) методом ИФА в 2015 – 2017 гг. показала, что на растениях картофеля сорта Ломоносовский скрытая зараженность составила 2,6 – 7,3 %, сорта Чародей — 1,9 – 13,3 %, сорта Невский — 0,8 – 10,5 %, сорта Романо — 4,1 – 9 %. Из известных переносчиков вирусов картофеля в оба года в энтомологических сборах с сосудов отмечены Aphis nasturtii — 6 – 12,2 %, Aphis fabae — 7,2 – 4,6 % от общего числа отловленных; Myzus persicae (13 %) в составе мигрирующей генерации тлей отмечены только в дождливом и прохладном 2017 г. В прогностических целях желательно пользоваться в регионе также данными со всасывающей ловушки, поскольку появление в ней насекомых отмечено на 10 – 12 дней раньше, чем на полях.


2018 ◽  
Author(s):  
Imran Rauf ◽  
Muhammad Asif ◽  
Imran Amin ◽  
Rubab Zahra Naqvi ◽  
Noroza Umer ◽  
...  

AbstractGut-expressed aphid genes, which may be more easily inhibited by RNA interference (RNAi) constructs, are attractive targets for pest control efforts involving transgenic plants. Here we show that expression of cathepsin L, a cysteine protease that functions in aphid guts, can be reduced by expression of an RNAi construct in transgenic tobacco. The effectiveness of this approach is demonstrated by up to 80% adult mortality, reduced fecundity, and delayed nymph production of Myzus persicae (green peach aphids) when cathepsin L expression was reduced by plant-mediated RNAi. Consistent with the function of cathepsin L as a gut protease, M. persicae fed on the RNAi plants had a lower protein content in their bodies and excreted more protein in their honeydew. Larvae of Coccinella septempunctata (seven-spotted ladybugs) grew more slowly on aphids having reduced cathepsin L expression, suggesting that prey insect nutritive value, and not just direct negative effects of the RNAi construct, needs to be considered when producing transgenic plants for RNAi-mediated pest control.HighlightsSilencing expression of cathepsin L by RNA interference reduces protein content of Myzus persicae (green peach aphid) bodies.Honeydew of aphids with cathepsin L silenced contains elevated protein.Cathepsin L is required for efficient protein uptake from phloem sap.Aphids with cathepsin L expression silenced have increased mortality and fewer offspring.Coccinella septempunctata (seven-spotted ladybugs) grow more slowly on aphids with expression of cathepsin L silenced.


1970 ◽  
Vol 27 ◽  
pp. 31-34
Author(s):  
M Khalequzzaman ◽  
Jesmun Nahar

Indirect application was used to assay the toxicity of five insecticides; viz. malathion, carbosulfan, cymbush, imidacloprid and azadirachtin against four important crop infesting aphid species, Aphis craccivora Koch, Aphis gossypii Glover, Myzus persicae (Sulzer) and Lipaphis erysimi (Kaltenbach), reared on bean, brinjal, potato and cauliflower plants respectively. Residual film technique was applied by bringing leaves with tested aphids of the vegetable plants in the laboratory. Malathion was the least toxic to all aphids having LC50 as 327.97, 333.92, 305.26 and 313.77 μg cm-2 for A. craccivora, A. gossypii, M. persicae and M. persicae respectively. Cypermethrin was the most toxic showing LC50 as 12.55, 12.29, 12.55 and 12.10 μg cm-2 in the above mentioned species of aphid respectively. Carbosulfan and imidacloprid showed moderate toxicity. Azadirachtin as a natural plant origin insecticide proved to be the most toxic having LC50 as 0.41 μg cm-2 for A. craccivora, 0.34 μg cm-2 for A. gossypii and 0.44 μg cm-2 for both M. persicae and L. erysimi. Key words: Insecticide, toxicity, azadirachtin, Aphis craccivora, Aphis gossypii, Myzus persicae, Lipaphis erysimi   doi:10.3329/ujzru.v27i0.1950 Univ. j. zool. Rajshahi Univ. Vol. 27, 2008 pp. 31-34


1974 ◽  
Vol 64 (4) ◽  
pp. 669-676 ◽  
Author(s):  
G. D. Heathcote

AbstractLong-term records of aphid catches on two sticky traps, one at Rothamsted (1942–64) and one at Broom's Barn (1960–64) are extended to 1973 and are supplemented by data from up to 12 sticky traps in sugar-beet crops in eastern England (1960–73). The incidence of yellowing viruses of sugar-beet between 1965 and 1973 showed no relation to aphid numbers, but between 1942 and 1973 the pattern of annual incidence was similar to that of numbers of Myzus persicae (Sulz.) trapped in May and June. Linear regressions of the incidence of yellowing viruses on numbers of M. persicae in May and June accounted for 28–57% of the variance, and on Aphis fabae group for 2–40% of the variance. It is concluded that sticky traps provide useful information for advisory entomologists and that there has been no general increase in aphid numbers over the last 30 years.


BioControl ◽  
2005 ◽  
Vol 50 (4) ◽  
pp. 565-588 ◽  
Author(s):  
Ioanna Katsarou ◽  
John T. Margaritopoulos ◽  
John A. Tsitsipis ◽  
Dionyssios Ch. Perdikis ◽  
Kostas D. Zarpas

2015 ◽  
Vol 95 (1) ◽  
pp. 27-31
Author(s):  
K. Shankarganesh ◽  
Sachin Suresh Suroshe ◽  
Bishwajeet Paul

A study was undertaken to assess the effectiveness of five insecticides against the Delhi and Bikaner populations of mustard aphid, Lipaphis erysimi (Kalt.), using the leaf dip method, and against Coccinella septempunctata L. in semi-field conditions. Acetamiprid and thiamethoxam were found to be more toxic than other insecticides. After 24 h, the LC50 values for the Bikaner population against different insecticides were 7.0, 6.0, 4.0, 3.0 and 2.0 ppm for carbosulfan, bifenthrin, imidacloprid, acetamiprid and thiamethoxam, respectively. Similarly, the descending order of toxicity for the Delhi population was acetamiprid (7.0 ppm), thiamethoxam (9.0 ppm), imidacloprid (15.0 ppm), carbosulfan (32.0 ppm) and bifenthrin (36.0 ppm). The relative toxicity values suggest that in both populations, thiamethoxam and acetamiprid show the highest toxicity. Carbosulfan and bifenthrin were highly toxic to coccinellid grubs and resulted in 100% mortality in semi-field conditions, whereas the neonicotinoids acetamiprid and thiamethoxam showed less mortality. It showed the tolerance of coccinellidae against neonicotinoids under semi-field conditions.


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