Bacterial Ribonuclease (Binase) Promotes Decontamination of MDBK Cell Cultures From Bovine Diarrhea Virus

2021 ◽  
Author(s):  
Marina A. Efimova ◽  
Antonina G. Mukhamedzhanova ◽  
Raihan Shah Mahmud ◽  
Nail I. Khammadov ◽  
Konstantin V. Usoltcev ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Mdbk Cell ◽  
Diarrhea Virus ◽  
Bovine Diarrhea Virus

scholarly journals Bovine Diarrhea Virus

1968 ◽  
Vol 12 (2) ◽  
pp. 201-210 ◽  
Author(s):  
Yoshio Tanaka ◽  
Yuji Inaba ◽  
Tuneyoshi Omori ◽  
Minoru Matumoto
Keyword(s):  
Diarrhea Virus ◽  
Bovine Diarrhea Virus

 Evaluating large spontaneous deletions in a bovine cell line selected for bovine viral diarrhea virus resistance - MDBK reads mapping to regions in genome  not shared with CRIB cell line v1

2021 ◽  
Author(s):  
aspen.workman not provided ◽  
mike.heaton not provided ◽  
Dennis A. Webster ◽  
Gregory P Harhay ◽  
Tim Smith ◽  
...  
Keyword(s):  
Cell Line ◽  
Bovine Viral Diarrhea Virus ◽  
Virus Entry ◽  
Mdbk Cell ◽  
Genomic Variation ◽  
Host Factors ◽  
Bovine Viral Diarrhea ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Viral Diarrhea Virus

Bovine viral diarrhea virus (BVDV) entry into bovine cells involves attachment of virions to cellular receptors, internalization, and pH-dependent fusion with endosomal membranes. The primary host receptor for BVDV is CD46; however, the complete set of host factors required for virus entry is unknown. The Madin-Darby bovine kidney (MDBK) cell line is susceptible to BVDV infection, while a derivative cell line (CRIB) is resistant at the level of virus entry. We performed complete genome sequencing of each to identify genomic variation underlying the resistant phenotype with the aim of identifying host factors essential for BVDV entry. Three large compound deletions in the BVDV-resistant CRIB cell line were identified and predicted to disrupt the function or expression of the genesPTPN12,GRID2, andRABGAP1L. However, CRISPR/Cas9 mediated knockout of these genes, individually or in combination, in the parental MDBK cell line did not impact virus entry or replication. Therefore, resistance to BVDV in the CRIB cell line is not due to the apparent spontaneous loss ofPTPN12,GRID2, orRABGAP1Lgene function. Identifying the functional cause of BVDV resistance in the CRIB cell line may require more detailed comparisons of the genomes and epigenomes.

scholarly journals Isolation and Serial Propagation of Porcine Epidemic Diarrhea Virus in Cell Cultures and Partial Characterization of the Isolate.

1992 ◽  
Vol 54 (2) ◽  
pp. 313-318 ◽  
Author(s):  
Ko-ichi KUSANAGI ◽  
Hiroyoshi KUWAHARA ◽  
Tetsuo KATOH ◽  
Tetsuo NUNOYA ◽  
Yoshihisa ISHIKAWA ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Porcine Epidemic Diarrhea Virus ◽  
Partial Characterization ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea ◽  
Serial Propagation

153 EFFECTS OF OVINE INTERFERON-β ON REPLICATION OF BOVINE VIRAL DIARRHEA VIRUS AND BOVINE HERPESVIRUS-1

2008 ◽  
Vol 20 (1) ◽  
pp. 156 ◽  
Author(s):  
P. K. Galik ◽  
J. A. Gard ◽  
T. S. Spencer ◽  
M. S. Marley ◽  
D. A. Stringfellow ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Cultures ◽  
Bovine Herpesvirus ◽  
Bovine Viral Diarrhea ◽  
Bovine Herpesvirus 1 ◽  
Diarrhea Virus ◽  
Viral Diarrhea ◽  
Mdbk Cells ◽  
Herpesvirus 1 ◽  
Viral Diarrhea Virus

Bovine viral diarrhea virus (BVDV) and bovine herpesvirus-1 (BHV-1) are the most commonly isolated viruses from abattoir-origin materials utilized in embryo production and known to associate with zona pellucida-intact (ZP-I) embryos after exposure and washing. Some evidence indicates that developing embryos may produce substances that are able to inhibit viral replication in adjacent cells. Interferons such as recombinant human interferon-α are known to have anti-BVDV activity but no effect against BHV-1. In some preliminary studies, bovine interferon-τ has shown antiviral activities against BVDV but not against BHV-1. However, interferon-τ in other species has not been evaluated for anti-BVDV and anti-BHV-1 effects. Thus, the objective of this study was to evaluate the cytotoxicity and anti-viral effect of ovine interferon-τ against a non-cytopathic high affinity strain of BVDV (SD-1) and BHV-1 (Colorado) in cell culture. Serial dilutions (1:10) beginning with an initial concentration of 1 mg mL–1 of interferon-τ were made in 96-well plates and then Madin Darby bovine kidney (MDBK) cells were seeded in the wells. Cells and interferon-τ were incubated at 37.5�C in 5% CO2 and air for 24 h prior to addition of virus. The following concentrations of BVDV were added to the wells: 6000, 3500, 1000, 625, and 350 cell culture infective doses (CCID50) (50% endpoint) per well. In addition, four viral concentrations of BHV-1, 1000, 500, 250, and 100 CCID50/mL were evaluated in separate cell cultures. Virus isolation was utilized to determine if the addition of interferon-τ decreased the amount of infective virus. Ovine interferon-τ produced no observable cytotoxicity in MDBK cells in any of the assays. Also, the three highest concentrations of interferon-τ significantly decreased the amount of BVDV in all of the concentrations of BVDV tested but had no apparent effect on the concentration of BHV-1 in cell cultures. Therefore ovine interferon-τ has anti-BVDV effects similar to those seen with bovine interferont-τ and neither has any apparent antiviral activity on BHV-1 in cell culture. Additionally, ovine and bovine interferon-τ might serve to limit or prevent the transmission of BVDV and curtail the negative effects of BVDV on oocyte and embryo development. However, a similar effect is not expected for BHV-1.

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