A probe-based quantitative PCR assay for detecting Tetracapsuloides bryosalmonae in fish tissue and environmental DNA water samples

2017 ◽  
Vol 10 (3) ◽  
pp. 317-319 ◽  
Author(s):  
Patrick R. Hutchins ◽  
Adam J. Sepulveda ◽  
Renee M. Martin ◽  
Lacey R. Hopper
Keyword(s):  
Quantitative Pcr ◽  
Water Samples ◽  
Environmental Dna ◽  
Fish Tissue ◽  
Pcr Assay ◽  
Tetracapsuloides Bryosalmonae ◽  
Quantitative Pcr Assay

scholarly journals Development of a Quantitative PCR Assay for Arcobacter spp. and its Application to Environmental Water Samples

2018 ◽  
Vol 33 (3) ◽  
pp. 309-316 ◽  
Author(s):  
Rajani Ghaju Shrestha ◽  
Yasuhiro Tanaka ◽  
Bikash Malla ◽  
Sarmila Tandukar ◽  
Dinesh Bhandari ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Water Samples ◽  
Environmental Water ◽  
Environmental Water Samples ◽  
Pcr Assay ◽  
Quantitative Pcr Assay

Development of a quantitative PCR assay for detecting Egeria densa in environmental DNA samples

2020 ◽  
Vol 12 (4) ◽  
pp. 545-548
Author(s):  
Dorothy M. Chase ◽  
Lauren M. Kuehne ◽  
Julian D. Olden ◽  
Carl O. Ostberg
Keyword(s):  
Quantitative Pcr ◽  
Environmental Dna ◽  
Egeria Densa ◽  
Pcr Assay ◽  
Quantitative Pcr Assay

scholarly journals Development of a Reverse Transcription-Quantitative PCR Assay for Detection of Salivirus/Klassevirus

2013 ◽  
Vol 79 (11) ◽  
pp. 3529-3532 ◽  
Author(s):  
Eiji Haramoto ◽  
Masaaki Kitajima ◽  
Mikie Otagiri
Keyword(s):  
Quantitative Pcr ◽  
Reverse Transcription ◽  
Water Samples ◽  
Maximum Concentration ◽  
Pcr Assay ◽  
River Water Samples ◽  
Treated Sewage ◽  
Reverse Transcription Quantitative Pcr ◽  
Highly Sensitive ◽  
Quantitative Pcr Assay

ABSTRACTA broadly reactive and highly sensitive reverse transcription-quantitative PCR assay to detect salivirus/klassevirus was developed. By means of the developed assay, salivirus/klassevirus was detected in 13 (93%) raw sewage, 4 (29%) secondary-treated sewage, and 9 (16%) river water samples, with a maximum concentration of 9.7 × 106copies/liter.

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