Development of a multiplex quantitative PCR assay for detection and quantification of DNA from Fasciola hepatica and the intermediate snail host, Austropeplea tomentosa, in water samples

2018 ◽  
Vol 259 ◽  
pp. 17-24 ◽  
Author(s):  
Vignesh Rathinasamy ◽  
Chris Hosking ◽  
Lily Tran ◽  
Jane Kelley ◽  
Genevieve Williamson ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Water Samples ◽  
Fasciola Hepatica ◽  
Snail Host ◽  
Pcr Assay ◽  
Intermediate Snail Host ◽  
Quantitative Pcr Assay ◽  
Detection And Quantification

scholarly journals Development of a Quantitative PCR Assay for Arcobacter spp. and its Application to Environmental Water Samples

2018 ◽  
Vol 33 (3) ◽  
pp. 309-316 ◽  
Author(s):  
Rajani Ghaju Shrestha ◽  
Yasuhiro Tanaka ◽  
Bikash Malla ◽  
Sarmila Tandukar ◽  
Dinesh Bhandari ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Water Samples ◽  
Environmental Water ◽  
Environmental Water Samples ◽  
Pcr Assay ◽  
Quantitative Pcr Assay

scholarly journals A short interspersed nuclear element-based quantitative PCR assay for simultaneous human and dog DNA detection and quantification

BioTechniques ◽  
2020 ◽  
Author(s):  
James W Liang ◽  
Heather Miller Coyle
Keyword(s):  
Quantitative Pcr ◽  
Positive Control ◽  
Direct Application ◽  
Pcr Assay ◽  
Forensic Casework ◽  
Internal Positive Control ◽  
Labeled Probe ◽  
High Degree ◽  
Quantitative Pcr Assay ◽  
Detection And Quantification

This study aimed to develop a quantitative PCR assay to simultaneously quantify human and dog DNA in a multispecies mixture to inform downstream analyses in routine forensic casework and scientific research. The human target is the Alu Yb8 element, which has approximately 2000 copies per cell, and the canine target is the SINEC_Cf element, which has approximately 200,000 copies per cell. The internal positive control is a universal exogenous assay consisting of forward and reverse primers, a NED-labeled probe with an MGBNFQ quencher and a 65-bp synthetic template. Results suggest a potentially robust assay with a fast run time and a high degree of sensitivity, precision and species specificity, with direct application to domestic pet samples in veterinary genetics and forensics.

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