Flow cytometric analysis of nuclear DNA content, mitotic chromosome number and protein separation by SDS-PAGE in three accessions of African locust bean (Parkia biglobosa Benth.)

2011 ◽  
Vol 14 (4) ◽  
pp. 227-232 ◽  
Author(s):  
Edak Aniedi Uyoh ◽  
Ikootobong Sunday Urua ◽  
Valentine Otang Ntui ◽  
Elza Cletus Okpako
1999 ◽  
Vol 98 (8) ◽  
pp. 1344-1350 ◽  
Author(s):  
M. A. Lysák ◽  
M. Dolez˘elová ◽  
J. P. Horry ◽  
R. Swennen ◽  
J. Dolez˘el

1979 ◽  
Vol 27 (1) ◽  
pp. 520-521 ◽  
Author(s):  
E Sprenger ◽  
S Witte

Flow cytometric analysis of cytologic samples from four different organs shows that nuclear DNA content of malignant cell populations depends to a large extent on organ of origin of the tumor. This fact must be considered in planning screening systems.


1995 ◽  
Vol 24 (1) ◽  
pp. 44-47 ◽  
Author(s):  
Tohru Saito ◽  
Tomomi Yamashita ◽  
Ken-ichi Notani ◽  
Hiroshi Fukuda ◽  
Shigetaka Mizuno ◽  
...  

1992 ◽  
Vol 38 (8) ◽  
pp. 1253-1256
Author(s):  
Tatsuo TSUJI ◽  
Kohsuke SASAKI ◽  
Yuka KIMURA ◽  
Tomoyuki MURAKAMI ◽  
Masamichi IDA ◽  
...  

1991 ◽  
Vol 69 (2) ◽  
pp. 239-244 ◽  
Author(s):  
Narender S. Nehra ◽  
Kutty K. Kartha ◽  
Cecil Stushnoff

Callus cultures of strawberry cv. Redcoat (2n = 8x = 56) initiated from greenhouse and in vitro leaf explants were examined at various culture periods for morphogenic response, changes in nuclear DNA content, and isozyme banding patterns of four enzymes. The flow cytometric analysis of nuclear DNA content revealed the occurrence of polyploid and aneuploid changes as a function of ageing of callus cultures. The calli initiated from in vitro leaf explants were more prone to such changes than those initiated from greenhouse leaf explants. The in vitro morphogenic ability of callus cultures was affected by the ploidy changes, but the latter were not the only cause for loss in regeneration potential of long-term callus cultures. The isozyme phenotypes of esterase, phosphoglucomutase, phosphoglucoisomerase, and leucine aminopeptidase did not change with the chromosomal variation in callus cultures. Key words: strawberry, Fragaria × ananassa, callus culture, flow cytometry, nuclear DNA content, isozyme.


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