Evaluation of carbon sources, gelling agents, growth hormones and additives for efficient callus induction and plant regeneration in Indian wheat (Triticum aestivum L.) genotypes using mature embryos

2017 ◽  
Vol 20 (3) ◽  
pp. 185-192 ◽  
Author(s):  
Kapil Malik ◽  
Deepshikha Birla ◽  
Honey Yadav ◽  
Manish Sainger ◽  
Darshna Chaudhary ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Callus Induction ◽  
Carbon Sources ◽  
Triticum Aestivum L ◽  
Growth Hormones ◽  
Gelling Agents ◽  
Mature Embryos

scholarly journals Callus Induction and Regeneration from Germinating Mature Embryos of Wheat (Triticum aestivum L.)

2021 ◽  
Vol 50 (4) ◽  
pp. 889-896
Author(s):  
Samih M. Tamimi ◽  
Halima Othman
Keyword(s):  
Triticum Aestivum ◽  
Callus Induction ◽  
High Performance ◽  
Triticum Aestivum L ◽  
Mature Embryo ◽  
Callus Cultures ◽  
Differentiation Potential ◽  
Mature Embryos ◽  
The Mean ◽  
Wheat Callus

A high-performance protocol for callus induction was devised using germinating mature embryos of two local wheat (Triticum aestivum L.) landraces as explant. The results showed that callus development from germinating embryos was rapid starting one day after culture with an induction rate 20 to 25% higher than those of soaked embryos. In addition, the mean rate of growth of callus developed from germinating embryos was 60 to 70% higher than those cultured from soaked embryos. This study also demonstrated a higher frequency of green spots formation(48 to 56%)on callus derived from germinating embryos compared to their soaked counterpart (24 to 28%), suggesting a better differentiation potential of callus cultures derived from germinating embryo. These findings indicate that germinating mature embryo is more suitable explant for wheat callus induction and regeneration than the soaked mature embryo commonly employed for wheat callus culture.

scholarly journals Effects of NaCl Stress on Callus Proliferation and Plant Regeneration from Mature Embryos of Bread Wheat (Triticum aestivum L.) Cultivars Mahon Demias and Hidhab

1970 ◽  
Vol 17 (1) ◽  
pp. 19-27 ◽  
Author(s):  
L Benderradji ◽  
H Bouzerzou ◽  
A Djekoun ◽  
A Benmahammed
Keyword(s):  
Triticum Aestivum ◽  
Bread Wheat ◽  
Salinity Stress ◽  
Callus Induction ◽  
Selection Process ◽  
Triticum Aestivum L ◽  
Nacl Stress ◽  
Mature Embryos ◽  
Callus Proliferation

An in vitro experiment with two cultivars, Mahon Demias and Hidhab of bread wheat (Triticum aestivum L.) exhibited appreciable callus induction but differed significantly in the capacity of calli proliferation and regeneration under salinity stress; even though Mahon Demias appeared relatively more tolerant than Hidhab. The results indicated the need to optimize a robust protocol for callus induction, maintenance and regeneration before selection process for tolerance to salinity is embarked upon.Key words: Bread wheat, Salinity stress, Callus proliferation, Mature embryoDOI = 10.3329/ptcb.v17i1.1117Plant Tissue Cult. & Biotech. 17(1): 19-27, 2007 (June)

Substitution analysis of callus induction and plant regeneration from anther culture in wheat (Triticum aestivum L.)

1988 ◽  
Vol 7 (2) ◽  
pp. 127-129 ◽  
Author(s):  
�va Szak�cs ◽  
G�za Kov�cs ◽  
J�nos Pauk ◽  
Be�ta Barnab�s
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Anther Culture ◽  
Callus Induction ◽  
Triticum Aestivum L
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