scholarly journals Effects of NaCl Stress on Callus Proliferation and Plant Regeneration from Mature Embryos of Bread Wheat (Triticum aestivum L.) Cultivars Mahon Demias and Hidhab

1970 ◽  
Vol 17 (1) ◽  
pp. 19-27 ◽  
Author(s):  
L Benderradji ◽  
H Bouzerzou ◽  
A Djekoun ◽  
A Benmahammed
Keyword(s):  
Triticum Aestivum ◽  
Bread Wheat ◽  
Salinity Stress ◽  
Callus Induction ◽  
Selection Process ◽  
Triticum Aestivum L ◽  
Nacl Stress ◽  
Mature Embryos ◽  
Callus Proliferation

An in vitro experiment with two cultivars, Mahon Demias and Hidhab of bread wheat (Triticum aestivum L.) exhibited appreciable callus induction but differed significantly in the capacity of calli proliferation and regeneration under salinity stress; even though Mahon Demias appeared relatively more tolerant than Hidhab. The results indicated the need to optimize a robust protocol for callus induction, maintenance and regeneration before selection process for tolerance to salinity is embarked upon.Key words: Bread wheat, Salinity stress, Callus proliferation, Mature embryoDOI = 10.3329/ptcb.v17i1.1117Plant Tissue Cult. & Biotech. 17(1): 19-27, 2007 (June)

scholarly journals Receiving of genetic-modified wheat plants with heterolous ornitin-δ-aminotransferase gene

2018 ◽  
Vol 22 ◽  
pp. 222-227
Author(s):  
O. M. Honcharuk ◽  
O. V. Dubrovna
Keyword(s):  
Triticum Aestivum ◽  
Transgenic Plants ◽  
Bread Wheat ◽  
Binary Vector ◽  
Triticum Aestivum L ◽  
Callus Cultures ◽  
Pcr Analysis ◽  
Genetic Construct ◽  
Agrobacterium Mediated Transformation

Aim. Receiving of genetically modified plants of bread wheat with heterologous ornithine‑δ‑aminotransferase gene. Methods. Agrobacterium-mediated transformation of callus cultures in vitro, PCR-analysis. Results. By Agrobacterium-mediated transformation of the morphogenic calluses of bread wheat (Triticum aestivum L.) using the AGLO strain containing the binary vector pBi-OAT with the target ornithine-δ-aminotransferase (oat) and selective neomycinphosphotransferase II (nptII), transgenic plants-regenerators have been obtained. Conclusions. As a result of the genetic transformation of Zimoyarka variety, 12 wheat regenerants were obtained in the genome which revealed a complete integration of the genetic construct containing the oat and nptII transgenes. Keywords: Triticum aestivum L., Agrobacterium-mediated transformation, ornithine‑δ‑aminotransferase gene, PCR-analysis.

scholarly journals Callus Induction and Regeneration from Germinating Mature Embryos of Wheat (Triticum aestivum L.)

2021 ◽  
Vol 50 (4) ◽  
pp. 889-896
Author(s):  
Samih M. Tamimi ◽  
Halima Othman
Keyword(s):  
Triticum Aestivum ◽  
Callus Induction ◽  
High Performance ◽  
Triticum Aestivum L ◽  
Mature Embryo ◽  
Callus Cultures ◽  
Differentiation Potential ◽  
Mature Embryos ◽  
The Mean ◽  
Wheat Callus

A high-performance protocol for callus induction was devised using germinating mature embryos of two local wheat (Triticum aestivum L.) landraces as explant. The results showed that callus development from germinating embryos was rapid starting one day after culture with an induction rate 20 to 25% higher than those of soaked embryos. In addition, the mean rate of growth of callus developed from germinating embryos was 60 to 70% higher than those cultured from soaked embryos. This study also demonstrated a higher frequency of green spots formation(48 to 56%)on callus derived from germinating embryos compared to their soaked counterpart (24 to 28%), suggesting a better differentiation potential of callus cultures derived from germinating embryo. These findings indicate that germinating mature embryo is more suitable explant for wheat callus induction and regeneration than the soaked mature embryo commonly employed for wheat callus culture.

Physiological and molecular response under salinity stress in bread wheat (Triticum aestivum L.)

2019 ◽  
Vol 29 (1) ◽  
pp. 125-133
Author(s):  
Priyanka Singh ◽  
Mahesh M. Mahajan ◽  
Nagendra Kumar Singh ◽  
Dinesh Kumar ◽  
Kanika Kumar
Keyword(s):  
Triticum Aestivum ◽  
Bread Wheat ◽  
Salinity Stress ◽  
Triticum Aestivum L ◽  
Molecular Response

scholarly journals Callus Induction, Proliferation, and Plantlets Regeneration of Two Bread Wheat (Triticum aestivum L.) Genotypes under Saline and Heat Stress Conditions

ISRN Agronomy ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Laid Benderradji ◽  
Faiçal Brini ◽  
Kamel Kellou ◽  
Nadia Ykhlef ◽  
Abdelhamid Djekoun ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Salt Stress ◽  
Bread Wheat ◽  
Heat Tolerance ◽  
Callus Induction ◽  
Thermal Stresses ◽  
Mature Embryo ◽  
Stress Conditions ◽  
Base Line

Response of two genotypes of bread wheat (Triticum aestivum), Mahon-Demias (MD) and Hidhab (HD1220), to mature embryo culture, callus production, and in vitro salt and heat tolerance was evaluated. For assessment of genotypes to salt and heat tolerance, growing morphogenic calli were exposed to different concentrations of NaCl (0, 5, 10, and 15 g·L−1) and under different thermal stress intensities (25, 30, 35, and 40°C). Comparison of the two genotypes was reported for callus induction efficiency from mature embryo. While, for salt and heat tolerance, the proliferation efficiency, embryonic efficiency, and regeneration efficiency were used. The results show significant medium and genotype effects for the embryogenesis capacity of calluses induction and plantlets regeneration under saline and thermal stresses. Mahon-Demias showed good callus induction and ability to proliferate and regenerate seedling under heat and salt stress conditions compared to Hidhab. No sizeable differences were observed between the two genotypes at higher salt stress rates. This study will serve as a base line for in vitro screening of several elite wheat cultivars for their ability to induce callus and regenerate plants from mature embryos, and to start selection for tolerance to salinity.

scholarly journals Effect of Ceftriaxone and Timentin antibiotics on morphogensis in the in vitro culture of bread wheat Triticum aestivum L.

2016 ◽  
Vol 32 (5) ◽  
pp. 367-376 ◽  
Author(s):  
M. A. Bannikova ◽  
I. R. Gorbatyuk ◽  
I. S. Hnatiuk ◽  
L. V. Malysheva-Otto ◽  
V. P. Duplij
Keyword(s):  
Triticum Aestivum ◽  
In Vitro Culture ◽  
Bread Wheat ◽  
Triticum Aestivum L

scholarly journals Tissues of immature and mature embryos as morphogenic competent explants for genetic transformation of wheat

2020 ◽  
Vol 26 ◽  
pp. 233-238
Author(s):  
S. I. Mykhalska ◽  
A. G. Komisarenko ◽  
V. M. Kurchii
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Molecular Genetic ◽  
Triticum Aestivum L ◽  
Callus Cultures ◽  
Molecular Genetic Analysis ◽  
Pcr Analysis ◽  
Agrobacterium Mediated Transformation ◽  
Mature Embryos

Aim. To analyze the efficiency of using tissues of immature and mature embryos for Agrobacterium-mediated transformation in vitro of new breeding-valuable genotypes of winter wheat (Triticum aestivum L.) for the purpose of their genetic improvement. Methods. Culture of in vitro, extraction and electrophoresis of DNA, PCR analysis. Results. The efficiency of induction of callusogenesis and regeneration of winter wheat shoots was analyzed. The morphogenetic response of callus cultures obtained from different explants under Agrobacterium-mediated transformation in vitro was investigated. Molecular genetic analysis of wheat regenerants for transgenes was performe. Conclusions. The tissues of immature and mature embryos of novel breeding-valuable wheat genotypes are competent explants for Agrobacterium-mediated transformation in vitro. In this case, the tissues of two daily sprouts of mature wheat germ are characterized by higher morphogenetic parameters, which helps to obtain a greater percentage of genetically modified variants. Keywords: Triticum aestivum L., in vitro, Agrobacterium-mediated transformation, immature embryos, mature embryos.

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