scholarly journals Optimization of medium composition for in vitro shoot proliferation and growth of date palm cv. Mejhoul

3 Biotech ◽  
2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Mouaad Amine Mazri ◽  
Reda Meziani ◽  
Jamal El Fadile ◽  
Az-eddine Ezzinbi
Keyword(s):  
Date Palm ◽  
Shoot Proliferation ◽  
Medium Composition ◽  
In Vitro Shoot Proliferation

Effectiveness of LED and fluorescent light on in vitro shoot proliferation ofStaphylea pinnata

2017 ◽  
pp. 375-380
Author(s):  
B. Szewczyk-Taranek ◽  
B. Pawłowska ◽  
B. Prokopiuk ◽  
M. Żupnik
Keyword(s):  
Shoot Proliferation ◽  
Fluorescent Light ◽  
In Vitro Shoot Proliferation

scholarly journals Effect of Media Types and Carbon Sources on In vitro Shoot Proliferation of Spilanthes acmella (L.) Murray

2014 ◽  
Vol 23 (2) ◽  
pp. 283-288
Author(s):  
Veenu Joshi ◽  
S. K. Jadhav
Keyword(s):  
Carbon Sources ◽  
Shoot Proliferation ◽  
Spilanthes Acmella ◽  
Media Types ◽  
In Vitro Shoot Proliferation

D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17529Plant Tissue Cult. & Biotech. 23(2): 283-288, 2013  (December)

scholarly journals In vitro propagation of six selected sugarcane mutant clones through leaf explants

2021 ◽  
Vol 883 (1) ◽  
pp. 012075
Author(s):  
R Purnamaningsih ◽  
D Sukmadjaja ◽  
S Suhesti ◽  
S Rahayu
Keyword(s):  
Callus Induction ◽  
Casein Hydrolysate ◽  
Leaf Explants ◽  
Shoot Proliferation ◽  
Medium Composition ◽  
Culture Techniques ◽  
High Productivity ◽  
Media Formulation ◽  
Number Of Shoots

Abstract Six mutant clones of sugarcane with high productivity have been produced through tissue culture techniques combined with mutations using gamma-ray irradiation and Ethyl Methane Sulfonate. The six mutant clones have been tested for stability in the field. They are proven to have high productivity and yields, so that they are very potential to be developed as superior varieties. To support the planting material sufficiency of these clones, an efficient propagation method was needed. Media formulations with different physical properties and composition of growth regulators were tested to obtain high seedling propagation rates. The media formulation for callus induction was Murashige dan Skoog (MS) + 3 mg/l 2,4-D + 3 g/l casein hydrolysate + 3% sucrose and for shoot regeneration was MS + 0,5 mg/l BA + 0,1 mg/l IBA + 100 mg/l PVP and 2% sucrose. Shoot proliferation was carried out on MS liquid (1, ½) + (0.3; 0.5 mg/l) BA + 0.1 mg/l IBA + 1 mg/l Kinetin + (0; 0.5 mg/l) GA3+ sucrose 2%. The results showed that callus induction, callus regeneration, and shoot proliferation of sugarcane mutant clones were influenced by the genotype and medium composition. The fastest callus induction was obtained from the MSP-4 clone (5.82 days), and the longest was MSB-7 (8.82 days). The largest callus diameter was obtained from MSB-6 clone on MS medium containing 1 mg/l BA, 100 mg/l PVP, and 2% sucrose. The highest number of shoots was obtained from the MSB-6 clone, while the least number of shoots conducted from the MSB-8 clone. The MSB-8 clones were more difficult to regenerate compared to the others. The best media formulation for shoot proliferation was ½ MS containing 0.5 mg/l BA, 1 mg/l Kinetin, and 0.1 mg/l IBA, while the best formulation for rooting was ½ MS.

scholarly journals In vitro shoot proliferation of Passiflora caerulea L. via cotyledonary node and shoot tip explants

2017 ◽  
Vol 2 ◽  
pp. 113-119 ◽  
Author(s):  
Marziyeh Jafari ◽  
Mohammad Hosein Daneshvar ◽  
Amin Lotfi
Keyword(s):  
Shoot Proliferation ◽  
Cotyledonary Node ◽  
Shoot Tip ◽  
Shoot Tip Explants ◽  
In Vitro Shoot Proliferation

scholarly journals In vitro Shoot Proliferation and Plant Regeneration of Phlogacanthus thyrsiflorus Nees. a Rare Medicinal Shrub of Bangladesh

2011 ◽  
Vol 21 (2) ◽  
pp. 135-141 ◽  
Author(s):  
A.K. M. Sayeed Hassan ◽  
Nadira Begum ◽  
Rebeka Sultana ◽  
Rahima Khatun
Keyword(s):  
Survival Rate ◽  
Plant Regeneration ◽  
Room Temperature ◽  
Shoot Proliferation ◽  
Nodal Explants ◽  
Shoot Induction ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
In Vitro Shoot Proliferation

An efficient protocol was developed for shoot proliferation and plant regeneration of Phlogacanthus thyrsiflorus Nees. (Acanthaceae) - a rare medicinal shrub of Bangladesh, through in vitro culture using shoot tip and nodal explants. Best shoot induction was observed on MS with 1.0 mg/l BAP + 0.5 mg/l NAA, in which 84.2% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture. In vitro raised shoots rooted on half-strength MS with 0.5 mg/l IBA + 0.5 mg/l NAA. For acclimation and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. Key words: Phlogacanthus thyrsiflorus, Shoot proliferation, Plant regeneration   D. O. I. 10.3329/ptcb.v21i2.10236   Plant Tissue Cult. & Biotech. 21(2): 135-141, 2011 (December)

scholarly journals In Vitro Shoot Proliferation and Rooting of Several Japanese Persimmon Cultivars

1989 ◽  
Vol 58 (1) ◽  
pp. 55-61 ◽  
Author(s):  
Hideki MURAYAMA ◽  
Ryutaro TAO ◽  
Tatsumi TANAKA ◽  
Akira SUGIURA
Keyword(s):  
Shoot Proliferation ◽  
Japanese Persimmon ◽  
In Vitro Shoot Proliferation

scholarly journals Does dikegulac affect in vitro shoot proliferation  and hyperhydricity incidence in olive explants?

2018 ◽  
Vol 45 (No. 3) ◽  
pp. 125-130
Author(s):  
Chrysovalantou Antonopoulou ◽  
Kortessa Dimassi Dimassi ◽  
Ioannis Therios Therios ◽  
Christos Chatzissavvidis
Keyword(s):  
Olea Europaea ◽  
Shoot Proliferation ◽  
Lateral Shoot ◽  
In Vitro Shoot Proliferation ◽  
Medium Dose ◽  
Olea Europaéa

Dikegulac was tested as a lateral shoot-inducing agent on micropropagated olive (Olea europaea ‘Chondrolia Chalkidikis’) shoots. Rugini olive medium was supplemented with dikegulac at 0, 16.9, 33.8, 66.7, 100.5 or 133.4 μΜ. Dikegulac was not phytotoxic and the explants treated with 100.5 μΜ had higher number, length, and weight of new shoots. Hyperhydricity (or vitrification) symptoms were diminished by increasing dikegulac concentration in the medium dose (66.7–133.4 μΜ). Also, dikegulac stimulated the production of large amounts of callus at the base of olive explants.  

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