scholarly journals Comparative assessment of four RNA extraction methods and modification to obtain high-quality RNA from Parthenium hysterophorus leaf

3 Biotech ◽  
2017 ◽  
Vol 7 (6) ◽  
Author(s):  
Javed Ahmad ◽  
M. Affan Baig ◽  
Arlene A. Ali ◽  
Asma Al-Huqail ◽  
M. M. Ibrahim ◽  
...  
Keyword(s):  
Rna Extraction ◽  
Extraction Methods ◽  
Comparative Assessment ◽  
Parthenium Hysterophorus ◽  
High Quality ◽  
Rna Extraction Methods

Comparative assessment of three RNA extraction methods for obtaining high-quality RNA from Candida viswanathii biomass

2021 ◽  
pp. 106200
Author(s):  
Micaele Rodrigues de Souza ◽  
Ronan Cristhian Teixeira ◽  
Matheus Martins Daúde ◽  
Anderson Neiva Lopes Augusto ◽  
Solange Aparecida Ságio ◽  
...  
Keyword(s):  
Rna Extraction ◽  
Extraction Methods ◽  
Comparative Assessment ◽  
High Quality ◽  
Candida Viswanathii ◽  
Rna Extraction Methods

scholarly journals Evaluation of different RNA extraction methods for high-quality total RNA and mRNA from Erwinia amylovora in planta

2016 ◽  
Vol 146 (4) ◽  
pp. 893-899 ◽  
Author(s):  
Monika Kałużna ◽  
Anita Kuras ◽  
Artur Mikiciński ◽  
Joanna Puławska
Keyword(s):  
Erwinia Amylovora ◽  
Rna Extraction ◽  
Extraction Methods ◽  
In Planta ◽  
High Quality ◽  
Total Rna ◽  
Rna Extraction Methods

scholarly journals Big data from small tissues: extraction of high-quality RNA for different plant tissue types during oilseed Brassica spp. seed development for RNA-Sequencing

2019 ◽  
Author(s):  
Laura Siles ◽  
Peter Eastmond ◽  
Smita Kurup
Keyword(s):  
Gene Expression ◽  
Rna Sequencing ◽  
Seed Development ◽  
Developmental Stages ◽  
Rna Extraction ◽  
Rna Isolation ◽  
Extraction Methods ◽  
High Quality ◽  
Gene Expression Analyses ◽  
Rna Extraction Methods

AbstractObtaining high-quality RNA for gene expression analyses from different seed tissues is challenging due to the presence of various contaminants, such as polyphenols, polysaccharides and lipids which interfere with RNA extraction methods. At present, the available protocols for extracting RNA from seeds require high amounts of tissue and are mainly focused on extracting RNA from whole seeds. However, extracting RNA at the tissue level enables more detailed studies regarding tissue specific transcriptome during development. Seeds from heart stage embryo to mature developmental stages of Brassica napus and B. oleracea were sampled for isolation of the embryo, endosperm and seed coat tissues. Ovules and gynoecia wall tissue were also collected at the pre-fertilization stage. After testing several RNA extraction methods, E.Z.N.A. Plant RNA Kit and Picopure RNA Isolation kit extraction methods with some modifications, as well as the use of PVPP for seed coats and endosperms at green stages, resulted in high RNA concentrations with clear 28S and 18S bands and high RIN values. Here, we present efficient and reliable RNA extraction methods for different genotypes of Brassica spp for different tissue types during seed development. The high-quality RNA obtained by using these methodologies is suitable for RNA-Sequencing and gene expression analyses.

scholarly journals Significant compartment‐specific impact of different RNA extraction methods and PCR assays on the sensitivity of Hepatitis E virus detection

2021 ◽  
Author(s):  
Patrick Behrendt ◽  
Birgit Bremer ◽  
Daniel Todt ◽  
Eike Steinmann ◽  
Michael Peter Manns ◽  
...  
Keyword(s):  
Hepatitis E Virus ◽  
Rna Extraction ◽  
Virus Detection ◽  
Hepatitis E ◽  
Extraction Methods ◽  
Rna Extraction Methods ◽  
Pcr Assays ◽  
Specific Impact

scholarly journals Comparison of RNA Extraction Methods for Molecular Analysis of Oral Cytology

2016 ◽  
Vol 50 (2) ◽  
pp. 108-115 ◽  
Author(s):  
Alves Mônica Ghislaine Oliveira ◽  
Mario Pérez-Sayáns ◽  
Maria-Elena Padín-Iruegas ◽  
Maria Dolores Reboiras-López ◽  
José Manuel Suarez-Peñaranda ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Rna Extraction ◽  
Extraction Methods ◽  
Rna Extraction Methods ◽  
Oral Cytology

scholarly journals Optimal RNA Extraction Methods and Development of Synthetic Clones for Seven Strawberry Viruses

2020 ◽  
Vol 26 (3) ◽  
pp. 170-178
Author(s):  
Sun-Jung Kwon ◽  
Ju-Yeon Yoon ◽  
In-Sook Cho ◽  
Bong-Nam Chung
Keyword(s):  
Rna Extraction ◽  
Extraction Methods ◽  
Rna Extraction Methods

scholarly journals Detection of Viable Cryptosporidium parvum in Soil by Reverse Transcription–Real-Time PCR Targetinghsp70mRNA

2011 ◽  
Vol 77 (18) ◽  
pp. 6476-6485 ◽  
Author(s):  
Zhanbei Liang ◽  
Ann Keeley
Keyword(s):  
Cryptosporidium Parvum ◽  
Reverse Transcription ◽  
Rna Binding ◽  
Direct Detection ◽  
Rna Extraction ◽  
Milk Powder ◽  
Extraction Methods ◽  
Content Type ◽  
Total Rna ◽  
Rna Extraction Methods

ABSTRACTExtraction of high-quality mRNA fromCryptosporidium parvumis a key step in PCR detection of viable oocysts in environmental samples. Current methods for monitoring oocysts are limited to water samples; therefore, the goal of this study was to develop a rapid and sensitive procedure forCryptosporidiumdetection in soil samples. The efficiencies of five RNA extraction methods were compared (mRNA extraction with the Dynabeads mRNA Direct kit after chemical and physical sample treatments, and total RNA extraction methods using the FastRNA Pro Soil-Direct, PowerSoil Total RNA, E.Z.N.A. soil RNA, and Norgen soil RNA purification kits) for the direct detection ofCryptosporidiumwith oocyst-spiked sandy, loamy, and clay soils by using TaqMan reverse transcription-PCR. The study also evaluated the presence of inhibitors by synthesis and incorporation of an internal positive control (IPC) RNA into reverse transcription amplifications, used different facilitators (bovine serum albumin, yeast RNA, salmon DNA, skim milk powder, casein, polyvinylpyrrolidone, sodium hexametaphosphate, andSalmonella entericaserovar Typhi) to mitigate RNA binding on soil components, and applied various treatments (β-mercaptoethanol and bead beating) to inactivate RNase and ensure the complete lysis of oocysts. The results of spiking studies showed thatSalmonellacells most efficiently relieved binding of RNA. With the inclusion ofSalmonelladuring extraction, the most efficient mRNA method was Dynabeads, with a detection limit of 6 × 102oocysts g−1of sandy soil. The most efficient total RNA method was PowerSoil, with detection limits of 1.5 × 102, 1.5 × 103, and 1.5 × 104C. parvumoocysts g−1soil for sandy, loamy, and clay samples, respectively.

Rapid Virus Detection Procedure for Molecular Tracing of Shellfish Associated with Disease Outbreaks

2007 ◽  
Vol 70 (4) ◽  
pp. 967-974 ◽  
Author(s):  
ANA MARIA de RODA HUSMAN ◽  
FROUKJE LODDER-VERSCHOOR ◽  
HAROLD H. J. L. van den BERG ◽  
FRANÇOISE S. LE GUYADER ◽  
HILDE van PELT ◽  
...  
Keyword(s):  
Extraction Method ◽  
Rna Extraction ◽  
Virus Detection ◽  
Disease Outbreaks ◽  
Extraction Methods ◽  
Virus Rna ◽  
Pathogenic Viruses ◽  
Rna Extraction Methods ◽  
Digestive Glands ◽  
Outbreak Situation

Detection of pathogenic viruses in oysters implicated in gastroenteritis outbreaks is often hampered by time-consuming, specialist virus extraction methods. Five virus RNA extraction methods were evaluated with respect to performance characteristics and sensitivity on artificially contaminated oyster digestive glands. The two most promising procedures were further evaluated on bioaccumulated and naturally contaminated oysters. The most efficient method was used to trace the source in an outbreak situation. Out of five RNA extraction protocols, PEG precipitation and the RNeasy Kit performed best with norovirus genogroup III–spiked digestive glands. Analyzing 24-h bioaccumulated oysters revealed a slightly better sensitivity with PEG precipitation, but the RNeasy Kit was less prone to concentrate inhibitors. The latter procedure demonstrated the presence of human noroviruses in naturally contaminated oysters and oysters implicated in an outbreak. In this outbreak, in four out of nine individually analyzed digestive glands, norovirus was detected. In one of the oysters and in one of the fecal samples of the clinical cases, identical norovirus strains were detected. A standard and rapid virus extraction method using the RNeasy Kit appeared to be most useful in tracing shellfish as the source in gastroenteritis outbreaks, and to be able to make effective and timely risk management decisions.

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