Valorization of agro-wastes for the biosynthesis and characterization of polyhydroxybutyrate by Bacillus sp. isolated from rice bran dumping yard

3 Biotech ◽  
2021 ◽  
Vol 11 (4) ◽  
Author(s):  
Sivakumar Krishnan ◽  
Gandhi Shree Chinnadurai ◽  
Kartik Ravishankar ◽  
Dhamodharan Raghavachari ◽  
Palani Perumal
Keyword(s):  
2018 ◽  
Vol 54 (4A) ◽  
pp. 115
Author(s):  
Đào Thị Thanh Xuân

The Bacillus sp. strain G4 isolated from termites gut can produce cellulase with high activity in rice bran medium after 72 h of incubation at 37 °C. The crude enzymewas collected by centrifugation at 6,000 rpmfor 15 minutes.  The precipitation of cellulasewith ammonium sulfate 60 – 90 % saturation and acetone at the concentration 60 % – 90 % was studied. Results showed that ammonium sulfate 90 % saturation gave cellulase with highest purification factor 8.87 but the yield was only 59.9 %, whereas acetone at 90 % concentration gave highest yield 83.57 % with purification factor 4.38. CMCase activity of cellulasepreparation obtained by acetone precipitation at 90 % was optimum at pH 7 and 60 °C. Furthermore, CMCase was stable at pH 6.0 - 7.0 and at temperature lower than 50 °C. The CMCase was activated by ion Ca2+ but inhibited by Co2+, Zn2+, Cu2+, Fe2+, and was not affected by Mg2+ and Ba2+. The CMCase was inhibited by high concentration of SDS while EDTA and Tween 80 played activated role.


Heliyon ◽  
2021 ◽  
pp. e07715
Author(s):  
Punam Thapa ◽  
Alina Thapa ◽  
Sujan Khadka ◽  
Sanjeep Sapkota ◽  
Om Prakash Panta ◽  
...  

2021 ◽  
Vol 49 ◽  
pp. 56-63
Author(s):  
Mostafa Elmahmoudy ◽  
Nora Elfeky ◽  
Pu Zhongji ◽  
Yue Zhang ◽  
Yongming Bao

2013 ◽  
Vol 6 (11) ◽  
pp. 876-883 ◽  
Author(s):  
MBS Donio ◽  
SFA Ronica ◽  
V Thanga Viji ◽  
S Velmurugan ◽  
J Adlin Jenifer ◽  
...  

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