Characterization of cadmium biosorption by inactive biomass of two cadmium-tolerant endophytic bacteria Microbacterium sp. D2-2 and Bacillus sp. C9-3

Characterization of Endophytic Bacillus Isolated from Shallot Root as Biocontrol of Bacterial Leaf Blight Disease. Endophytic Bacillus isolated from the roots of healthy shallots has potential as biological control agent of bacterial leaf blight (BLB) disease. Based on the in planta screening, four endophytic bacteria that capable of controlling BLB diseases from the group of Bacillus were obtained, such as Bacillus cereus P14, Bacillus cereus Se07, Bacillus sp. HI, and Bacillus sp. SJI. The aims of this study were to investigate the characteristics of endophytic Bacillus that capable of controlling BLB disease and to determine the characteristic that most responsible in the disease control. This study method was descriptive. The characteristics observed were production of antibiotics by paper disc method, production of salicylic acid by capillary electrophoresis, and root colonization by in planta method. Linear regression analysis was used to determine the characteristic most responsible in the disease control. The results showed that four endophytic Bacillus were capable of producing salicylic acid and colonizing the root, and three of them were also capable of producing antibiotic. Salicylic acid production varied ranging from 13.96 to 14.72 ppm mL-1. Three endophytic Bacillus were capable of producing antibiotic with inhibition zone of 16.25 to 20.25 mm. Endophytic Bacillus was able to colonize the shallot roots with a population of bacteria ranged from 3.20 × 105-6, 20 × 105 CFU g-1 root. Based on the correlation coefficient of linear regression analysis, the root colonization of endophytic bacteria played the role in decreasing BLB disease.

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Short Communication: Isolation and characterization of the endophytic bacteria, and their potential as maize diseases control

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d210506 ◽

2020 ◽

Vol 21 (5) ◽

Author(s):

Endang Mugiastuti ◽

SUPRAYOGI ◽

NUR PRIHATININGSIH ◽

LOEKAS SOESANTO

Keyword(s):

Biological Control ◽

Bacterial Wilt ◽

Endophytic Bacteria ◽

Short Communication ◽

Plant Protection ◽

Mechanical Test ◽

Plant Growth Promoting Bacteria ◽

Bacillus Sp ◽

Isolation And Characterization

Abstract. Mugiastuti E, Suprayogi, Prihatiningsih N, Soesanto L. 2020. Short Communication: Isolation And Characterization Of The Endophytic Bacteria, And Their Potential As Maize Diseases Control. Biodiversitas 21: 1809-1815. Sheath blight and bacterial wilt are diseases that can reduce maize production. Biological control with the endophytic bacteria offers environmentally friendly control for these pathogens. The study aimed to isolate and characterize the endophytic bacteria morphologically and biochemically and to study their potential to control maize diseases, especially sheat blight and bacterial wilt causing pathogens. The study was conducted at the Plant Protection Laboratory, Faculty of Agriculture, Jenderal Soedirman University, from April to August 2019. The study consisted of four stages: isolation and characterization of endophytic bacteria, the antagonism test of the endophytic bacterial to R solani, the antagonism test of the endophytic bacteria to Pantoea sp., and the mechanical test of the endophytic bacteria as biological control agents and plant growth-promoting bacteria. Based on the research, four endophytic bacteria isolates have been successfully isolated, and characterized successfully and found have the potential to be developed as biopesticides to control maize disease, especially R. solani and Pantoea sp. Bacillus sp, endophytic from the root (BK.A1; BK.A3; PP.A5) and Bacillus sp. endophytic from the stem (PPD.B2) can suppress the growth of R.solani by more than 50%, have a strong antagonistic index against Pantoea sp (> 4), and can produce protease and lipase enzyme, and phosphate solubilization.

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