Establishment and characterization of NCC-ssRMS2-C1: a novel patient-derived cell line of spindle cell/sclerosing rhabdomyosarcoma

Human Cell ◽  
2021 ◽  
Author(s):  
Ryuto Tsuchiya ◽  
Yuki Yoshimatsu ◽  
Rei Noguchi ◽  
Yooksil Sin ◽  
Takuya Ono ◽  
...  
Keyword(s):  
Cell Line ◽  
Spindle Cell ◽  
Sclerosing Rhabdomyosarcoma

Establishment and characterization of NCC-ssRMS1-C1: a novel patient-derived spindle-cell/sclerosing rhabdomyosarcoma cell line

Human Cell ◽  
2020 ◽  
Vol 33 (3) ◽  
pp. 886-893 ◽  
Author(s):  
Yuki Yoshimatsu ◽  
Rei Noguchi ◽  
Ryuto Tsuchiya ◽  
Akane Sei ◽  
Jun Sugaya ◽  
...  
Keyword(s):  
Cell Line ◽  
Spindle Cell ◽  
Rhabdomyosarcoma Cell Line ◽  
Rhabdomyosarcoma Cell ◽  
Sclerosing Rhabdomyosarcoma

Establishment and characterization of a spindle cell squamous carcinoma cell line

2006 ◽  
Vol 35 (8) ◽  
pp. 479-483 ◽  
Author(s):  
Yasusei Kudo ◽  
Ikuko Ogawa ◽  
Masae Kitagawa ◽  
Shojiro Kitajima ◽  
B. S. M. Samadarani Siriwardena ◽  
...  
Keyword(s):  
Cell Line ◽  
Spindle Cell ◽  
Carcinoma Cell ◽  
Squamous Carcinoma ◽  
Carcinoma Cell Line ◽  
Squamous Carcinoma Cell

scholarly journals Establishment and Characterization of a Cell Line (S-RMS1) Derived from an Infantile Spindle Cell Rhabdomyosarcoma with SRF-NCOA2 Fusion Transcript

2021 ◽  
Vol 22 (11) ◽  
pp. 5484
Author(s):  
Marta Colletti ◽  
Angela Galardi ◽  
Evelina Miele ◽  
Virginia Di Paolo ◽  
Ida Russo ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Cell Line ◽  
Genome Sequencing ◽  
Gene Fusion ◽  
Spindle Cell ◽  
Population Doubling ◽  
Genetic Profile ◽  
Whole Genome ◽  
A Cell

Background: Spindle cell rhabdomyosarcoma (S-RMS) is a rare tumor that was previously considered as an uncommon variant of embryonal RMS (ERMS) and recently reclassified as a distinct RMS subtype with NCOA2, NCOA1, and VGLL2 fusion genes. In this study, we established a cell line (S-RMS1) derived from a four-month-old boy with infantile spindle cell RMS harboring SRF-NCOA2 gene fusion. Methods: Morphological and molecular characteristics of S-RMS1 were analyzed and compared with two RMS cell lines, RH30 and RD18. Whole genome sequencing of S-RMS1 and clinical exome sequencing of genomic DNA were performed. Results: S-RMS1 showed cells small in size, with a fibroblast-like morphology and positivity for MyoD-1, myogenin, desmin, and smooth muscle actin. The population doubling time was 3.7 days. Whole genome sequencing demonstrated that S-RMS1 retained the same genetic profile of the tumor at diagnosis. A Western blot analysis showed downregulation of AKT-p and YAP-p while RT-qPCR showed upregulation of endoglin and GATA6 as well as downregulation of TGFßR1 and Mef2C transcripts. Conclusion: This is the first report of the establishment of a cell line from an infantile spindle cell RMS with SRF-NCOA2 gene fusion. S-RMS1 should represent a useful tool for the molecular characterization of this rare and almost unknown tumor.

Characterization of oxytocin receptor in a human granulosa cell line

1998 ◽  
Vol 5 (1) ◽  
pp. 106A-106A
Author(s):  
J COPLAND ◽  
M ZLATMK ◽  
K IVES ◽  
M SOLOFF
Keyword(s):  
Cell Line ◽  
Granulosa Cell ◽  
Oxytocin Receptor ◽  
Human Granulosa Cell

Establishment and Characterization of a Human Glioblastoma Cell Line Which Expresses Somatostatin and its Functional Receptor

1999 ◽  
Vol 11 (3) ◽  
pp. 215-222
Author(s):  
Nobuo HIROTA ◽  
Kiyoshi MATSUMOTO ◽  
Shigeki SUNAGA ◽  
Masataka IIDA ◽  
Hiroshi SAKAGAMI ◽  
...  
Keyword(s):  
Cell Line ◽  
Glioblastoma Cell Line ◽  
Glioblastoma Cell ◽  
Human Glioblastoma ◽  
Human Glioblastoma Cell Line ◽  
Human Glioblastoma Cell ◽  
Functional Receptor

Characterization of a bovine intestinal myofibroblast cell line and stimulation using phytoglycogen-based nanoparticles bound to inosine monophosphate

2021 ◽  
Vol 57 (1) ◽  
pp. 86-94
Author(s):  
K. Jenik ◽  
T. N. Alkie ◽  
E. Moore ◽  
J. D. Dejong ◽  
L. E. J. Lee ◽  
...  
Keyword(s):  
Cell Line ◽  
Inosine Monophosphate
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