Automated Ribotyping and Pulsed-Field Gel Electrophoresis Reveal a Cluster of Group A Streptococci in Intravenous Drug Abusers

Infection ◽  
2000 ◽  
Vol 28 (5) ◽  
pp. 314-317 ◽  
Author(s):  
S. Brunner ◽  
F. Fleisch ◽  
C. Ruef ◽  
M. Altwegg
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Intravenous Drug ◽  
Drug Abusers ◽  
Group A Streptococci ◽  
Pulsed Field ◽  
Group A ◽  
Automated Ribotyping ◽  
Intravenous Drug Abusers

scholarly journals Typing of Group A Streptococci by Pulsed Field Gel Electrophoresis

1996 ◽  
Vol 70 (3) ◽  
pp. 283-286 ◽  
Author(s):  
Daisuke TANAKA ◽  
Yotaku GYOBU ◽  
Hirohide KODAMA
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Group A Streptococci ◽  
Pulsed Field ◽  
Group A

scholarly journals Genetic relatedness of group A streptococci of the newly designated serotype M90 causing a food-borne outbreak and sporadic infections

2000 ◽  
Vol 125 (2) ◽  
pp. 299-301 ◽  
Author(s):  
S. POURNARAS ◽  
A. EFSTRATIOU ◽  
J. DOUBOYAS ◽  
R. C. GEORGE ◽  
A. TSAKRIS
Keyword(s):  
Gel Electrophoresis ◽  
Genetic Relatedness ◽  
Group A Streptococcus ◽  
Pulsed Field Gel Electrophoresis ◽  
Group A Streptococci ◽  
Pulsed Field ◽  
Food Borne ◽  
Group A ◽  
Pfge Profiles

Twenty-six isolates of the newly designated M90 serotype group A Streptococcus (GAS) from a large food-borne outbreak of pharyngitis in Greece and six M90 sporadic isolates from UK, were typed by pulsed-field gel electrophoresis (PFGE). Twenty-four outbreak isolates were identical and two closely related. The Greek isolates were possibly related with one UK isolate, while other sporadic isolates exhibited distinct PFGE profiles from the former isolates.

scholarly journals Emergence of Macrolide Resistance in Throat Culture Isolates of Group A Streptococci in Ontario, Canada, in 2001

2003 ◽  
Vol 47 (7) ◽  
pp. 2370-2372 ◽  
Author(s):  
Kevin C. Katz ◽  
Allison J. McGeer ◽  
Carla L. Duncan ◽  
Aisha Ashi-Sulaiman ◽  
Barbara M. Willey ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Macrolide Resistance ◽  
Group A Streptococci ◽  
Throat Culture ◽  
Pulsed Field ◽  
Group A

ABSTRACT Of 500 group A streptococci isolated from pharyngeal swabs, 72 (14.4%) were macrolide resistant, compared to 2.1% in 1997. Of these, 66 (92%) were of the M phenotype and 6 (8.3%) were of the MLS phenotype. Pulsed-field gel electrophoresis found that two clones, with patterns identical to those of serotypes M1 and M4, accounted for 19.4 and 68.1% of the macrolide-resistant isolates, respectively.

A Comparison ofListeria monocytogenesSerovar 4b Isolates of Clinical and Food Origin in Austria by Automated Ribotyping and Pulsed-Field Gel Electrophoresis

2006 ◽  
Vol 3 (1) ◽  
pp. 138-141 ◽  
Author(s):  
Katharina Grif ◽  
Ingrid Heller ◽  
Martin Wagner ◽  
Manfred Dierich ◽  
Reinhard Würzner
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Automated Ribotyping

scholarly journals Genetic Features of Streptococcus agalactiae Strains Causing Severe Neonatal Infections, as Revealed by Pulsed-Field Gel Electrophoresis and hylB Gene Analysis

1999 ◽  
Vol 37 (6) ◽  
pp. 1892-1898 ◽  
Author(s):  
Karine Rolland ◽  
Corinne Marois ◽  
Veronique Siquier ◽  
Blandine Cattier ◽  
Roland Quentin
Keyword(s):  
Streptococcus Agalactiae ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pcr Analysis ◽  
Enzyme Electrophoresis ◽  
Invasive Infection ◽  
Gene Encoding ◽  
Pulsed Field ◽  
Asymptomatic Patients ◽  
Group A

A collection of 114 independent Streptococcus agalactiae strains, including 54 strains isolated from the cerebrospinal fluid (CSF) samples of neonates and 60 strains from asymptomatic patients, was characterized by pulsed-field gel electrophoresis (PFGE) of DNA restricted with SmaI and by PCR analysis of the hylB gene. All strains were previously studied by multilocus enzyme electrophoresis (MLEE) (R. Quentin, H. Huet, F.-S. Wang, P. Geslin, A. Goudeau, and R. K. Selander, J. Clin. Microbiol. 33:2576–2581, 1995). Among these 114 strains, there were 92 PFGE patterns. Eleven genetic groups (A to K) were identified with 38% divergence. A more homogeneous group (PFGE group A) was defined, consisting of 73% of the strains previously identified as belonging to a particular MLEE phylogenetic group. A 162-kb fragment was identified as a marker of strains that invaded the central nervous system of neonates. It was detected in 69% of the PFGE patterns obtained with CSF isolates and in only 1.8% of the PFGE patterns obtained with carrier strains. The hylB gene encoding hyaluronate lyase was amplified for all strains in our collection. Ten of 15 isolates belonging to an MLEE subgroup, previously described as being likely to cause invasive infection, had an insertion in the hylB gene (IS1548).

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