Pulsed-field gel electrophoresis comparison of pharyngeal and sterile site group A streptococcal isolates

2002 ◽  
Keyword(s):  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pulsed Field ◽  
Site Group ◽  
Group A ◽  
Sterile Site

scholarly journals Genetic Features of Streptococcus agalactiae Strains Causing Severe Neonatal Infections, as Revealed by Pulsed-Field Gel Electrophoresis and hylB Gene Analysis

1999 ◽  
Vol 37 (6) ◽  
pp. 1892-1898 ◽  
Author(s):  
Karine Rolland ◽  
Corinne Marois ◽  
Veronique Siquier ◽  
Blandine Cattier ◽  
Roland Quentin
Keyword(s):  
Streptococcus Agalactiae ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Pcr Analysis ◽  
Enzyme Electrophoresis ◽  
Invasive Infection ◽  
Gene Encoding ◽  
Pulsed Field ◽  
Asymptomatic Patients ◽  
Group A

A collection of 114 independent Streptococcus agalactiae strains, including 54 strains isolated from the cerebrospinal fluid (CSF) samples of neonates and 60 strains from asymptomatic patients, was characterized by pulsed-field gel electrophoresis (PFGE) of DNA restricted with SmaI and by PCR analysis of the hylB gene. All strains were previously studied by multilocus enzyme electrophoresis (MLEE) (R. Quentin, H. Huet, F.-S. Wang, P. Geslin, A. Goudeau, and R. K. Selander, J. Clin. Microbiol. 33:2576–2581, 1995). Among these 114 strains, there were 92 PFGE patterns. Eleven genetic groups (A to K) were identified with 38% divergence. A more homogeneous group (PFGE group A) was defined, consisting of 73% of the strains previously identified as belonging to a particular MLEE phylogenetic group. A 162-kb fragment was identified as a marker of strains that invaded the central nervous system of neonates. It was detected in 69% of the PFGE patterns obtained with CSF isolates and in only 1.8% of the PFGE patterns obtained with carrier strains. The hylB gene encoding hyaluronate lyase was amplified for all strains in our collection. Ten of 15 isolates belonging to an MLEE subgroup, previously described as being likely to cause invasive infection, had an insertion in the hylB gene (IS1548).

scholarly journals Comparison of pulsed-field gel electrophoresis and ribotyping for subtyping ofVibrio choleraeO139 isolated in Thailand

1996 ◽  
Vol 117 (1) ◽  
pp. 51-58 ◽  
Author(s):  
A. Dalsgaard ◽  
M. N. Skov ◽  
O. Serichantalergs ◽  
P. Echeverria
Keyword(s):  
Vibrio Cholerae ◽  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Pulsed Field Gel Electrophoresis ◽  
Evolutionary Divergence ◽  
Pulsed Field ◽  
Group A ◽  
Group B

SummaryPulsed-field gel electrophoresis (PFGE) ofCpoI-digested genomic DNA and ribotyping (BglI) were applied to 60Vibrio choleraestrains including 48V. choleraeO139 from Thailand to compare their value in differentiating strains of the presentV. choleraeO139 epidemic. PFGE patters were divided into groups A and B representing five and four subtypes, respectively, while ribotyping showed four different patterns. PFGE group B subtypes were only presented among O139 isolates from Thailand, whereas four O139 strains from Bangladesh and India showed identical PFGE group A subtypes observed in O139 isolates from Thailand. Two nontoxigenic O139 isolates from Thailand showed different and unique PFGE types as did fiveV. choleraenon-Ol non-O139 isolates containing a gene virulence complex found inV. choleraeO139. These results indicate that PFGE (CpoI) can resolve recent evolutionary divergence withinV. choleraeO139 and offers a useful supplementary tool for following the progressingV. choleraeO139 epidemic.

scholarly journals Genetic relatedness of group A streptococci of the newly designated serotype M90 causing a food-borne outbreak and sporadic infections

2000 ◽  
Vol 125 (2) ◽  
pp. 299-301 ◽  
Author(s):  
S. POURNARAS ◽  
A. EFSTRATIOU ◽  
J. DOUBOYAS ◽  
R. C. GEORGE ◽  
A. TSAKRIS
Keyword(s):  
Gel Electrophoresis ◽  
Genetic Relatedness ◽  
Group A Streptococcus ◽  
Pulsed Field Gel Electrophoresis ◽  
Group A Streptococci ◽  
Pulsed Field ◽  
Food Borne ◽  
Group A ◽  
Pfge Profiles

Twenty-six isolates of the newly designated M90 serotype group A Streptococcus (GAS) from a large food-borne outbreak of pharyngitis in Greece and six M90 sporadic isolates from UK, were typed by pulsed-field gel electrophoresis (PFGE). Twenty-four outbreak isolates were identical and two closely related. The Greek isolates were possibly related with one UK isolate, while other sporadic isolates exhibited distinct PFGE profiles from the former isolates.

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