Efficient In Vitro Regeneration System for Tecoma stans L., Using Shoot Tip and Assessment of Genetic Fidelity Among Regenerants

2019 ◽  
Vol 90 (1) ◽  
pp. 171-178 ◽  
Author(s):  
Sheikh Altaf Hussain ◽  
Mohammad Anis ◽  
Abdulrahman A. Alatar
Keyword(s):  
In Vitro Regeneration ◽  
Genetic Fidelity ◽  
Shoot Tip ◽  
Regeneration System

Establishment of in vitro regeneration system for Acaciella angustissima (Timbe) a shrubby plant endemic of México for the production of phenolic compounds

2016 ◽  
Vol 86 ◽  
pp. 49-57 ◽  
Author(s):  
Jannette Alonso-Herrada ◽  
Félix Rico-Reséndiz ◽  
Juan Campos-Guillén ◽  
Ramón G. Guevara-González ◽  
Irineo Torres-Pacheco ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
In Vitro Regeneration ◽  
Regeneration System

scholarly journals A rapid and efficient in vitro regeneration system for lettuce (Lactuca sativa L.)

Plant Methods ◽  
2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Isabel Armas ◽  
Natalia Pogrebnyak ◽  
Ilya Raskin
Keyword(s):  
Lactuca Sativa ◽  
In Vitro Regeneration ◽  
Regeneration System ◽  
Lactuca Sativa L

scholarly journals CLONAL PROPAGATION OF NEEM (Azadirachta indica A. Juss.) VIA DIRECT AND INDIRECT IN VITRO REGENERATION

2015 ◽  
Vol 39 (3) ◽  
pp. 439-445 ◽  
Author(s):  
Laureen Michelle Houllou ◽  
Robson Antônio de Souza ◽  
Elizabete Cristina Pacheco dos Santos ◽  
José Jackson Pereira da Silva ◽  
Marta Ribeiro Barbosa ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Callus Formation ◽  
In Vitro Regeneration ◽  
Regenerative Process ◽  
Shoot Tips ◽  
Shoot Tip ◽  
Stem Base ◽  
Different Characteristics

ABSTRACTThe study was conducted with shoot tip explants of neem (Azadirachta indica A. Juss) to identify a viable regenerative process. Shoot tips were obtained from neem embryos cultured alternatingly in DKW medium supplemented with BAP and medium without hormones. Initial shoot development was influenced by cotyledon presence. Basal callus, excised from in vitro stem base, also presented organogenic potential. In some cases, plant lines, obtained from each seed, presented different characteristics. The most common characteristic observed in vitro was callus formation at the stem base. However, the rarest characteristics were stem callus formation and leaf senescence. The regenerated shoot tips were further subculture and rooted on a medium supplemented with IBA so that complete plants could be obtained. The rooted plants were transplanted to a greenhouse and successfully acclimatized. No significant differences in in vivo development were observed between neem plants from callus and from shoot tip propagation.

scholarly journals In vitro regeneration protocol of Rauvolfia serpentina L.

2018 ◽  
Vol 53 (2) ◽  
pp. 133-138 ◽  
Author(s):  
S Khan ◽  
TA Banu ◽  
S Akter ◽  
B Goswami ◽  
M Islam ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Regeneration System ◽  
Rauvolfia Serpentina ◽  
Number Of Shoots

An efficient in vitro regeneration system was developed for Rauvolfia serpentina L. through direct and indirect organogenesis from nodal and leaf explants. Among the different growth regulators, MS medium supplemented with 2.0 mg/l BAP, 0.5mg/l IAA and 0.02mg/l NAA found best for the multiple shoot formation from nodal segments. In this combination 98% explants produced multiple shoots and the average number of shoots per explants is 13∙4. The frequency of callus induction and multiple shoot induction from leaves was highest 88% in MS medium supplemented with 2.0 mg/l BAP, where mean number of shoots/explants was 12.5. The highest frequency of root induction (80%) and mean number of roots/plantlets (10) were obtained on half strength of MS medium containing 0.2 mg/l IBA. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully established in the field.Bangladesh J. Sci. Ind. Res.53(2), 133-138, 2018

Evaluation of the Enhanced Regeneration System for in vitro regeneration in barley

2006 ◽  
Vol 86 (1) ◽  
pp. 63-69
Author(s):  
Seedhabadee Ganeshan ◽  
Brian J Weir ◽  
Monica Båga ◽  
Brian G Rossnagel ◽  
Ravindra N Chibbar
Keyword(s):  
Hordeum Vulgare ◽  
Embryogenic Callus ◽  
Culture Media ◽  
In Vitro Regeneration ◽  
Cold Treatment ◽  
Regeneration System ◽  
Embryogenic Calli ◽  
Hordeum Vulgare L ◽  
Best Response

A simple two-step model for evaluation of in vitro regeneration protocols is proposed based on callus induction and regeneration from immature scutella of two Canadian barley (Hordeum vulgare L.) genotypes, AC Metcalfe and SB92559 using the Enhanced Regeneration System (ERS). The number of explants producing embryogenic callus, the number of plants per embryogenic callus and the number of plants per explant were considered. Tissue culture parameters included three combinations of growth regulators, two carbon sources in culture media, and three cold treatment regimes of spikes prior to scutella isolation. Culture medium containing 5 µM 2,4-dichlorophenoxy acetic acid (2,4-D) and 0.5 µM benzyl adenine (BA) induced the highest percent of embryogenic calli and the highest number of shoots per embryogenic callus from AC Metcalfe. Medium containing 3.75 µM 2,4-D and 0.75 µM BA gave the best response for SB92559. Both genotypes produced more shoots on maltose than on sucrose medium. A 2-d treatment of spikes at 4°C resulted in best response for SB92559. Regeneration response from AC Metcalfe scutella from spikes was unaffected by being subjected to 2, 4 or 6 d of cold. Conditions resulting in best responses from both genotypes were tested on four commercial barley varieties. However, these lines showed inferior regeneration compared to SB92559 and AC Metcalfe. Key words: Hordeum vulgare, scutella, embryogenic callus, shoot production

Effective and simple in vitro regeneration system of Miscanthus sinensis , M . × giganteus and M . sacchariflorus for planting and biotechnology purposes

2017 ◽  
Vol 107 ◽  
pp. 219-226 ◽  
Author(s):  
Aurelia Ślusarkiewicz-Jarzina ◽  
Aleksandra Ponitka ◽  
Joanna Cerazy-Waliszewska ◽  
Maria Katarzyna Wojciechowicz ◽  
Karolina Sobańska ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Miscanthus Sinensis ◽  
Regeneration System
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