scholarly journals A rapid and efficient in vitro regeneration system for lettuce (Lactuca sativa L.)

Plant Methods ◽  
2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Isabel Armas ◽  
Natalia Pogrebnyak ◽  
Ilya Raskin
Keyword(s):  
Lactuca Sativa ◽  
In Vitro Regeneration ◽  
Regeneration System ◽  
Lactuca Sativa L

Establishment of in vitro regeneration system for Acaciella angustissima (Timbe) a shrubby plant endemic of México for the production of phenolic compounds

2016 ◽  
Vol 86 ◽  
pp. 49-57 ◽  
Author(s):  
Jannette Alonso-Herrada ◽  
Félix Rico-Reséndiz ◽  
Juan Campos-Guillén ◽  
Ramón G. Guevara-González ◽  
Irineo Torres-Pacheco ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
In Vitro Regeneration ◽  
Regeneration System

scholarly journals In vitro regeneration protocol of Rauvolfia serpentina L.

2018 ◽  
Vol 53 (2) ◽  
pp. 133-138 ◽  
Author(s):  
S Khan ◽  
TA Banu ◽  
S Akter ◽  
B Goswami ◽  
M Islam ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Multiple Shoot ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Regeneration System ◽  
Rauvolfia Serpentina ◽  
Number Of Shoots

An efficient in vitro regeneration system was developed for Rauvolfia serpentina L. through direct and indirect organogenesis from nodal and leaf explants. Among the different growth regulators, MS medium supplemented with 2.0 mg/l BAP, 0.5mg/l IAA and 0.02mg/l NAA found best for the multiple shoot formation from nodal segments. In this combination 98% explants produced multiple shoots and the average number of shoots per explants is 13∙4. The frequency of callus induction and multiple shoot induction from leaves was highest 88% in MS medium supplemented with 2.0 mg/l BAP, where mean number of shoots/explants was 12.5. The highest frequency of root induction (80%) and mean number of roots/plantlets (10) were obtained on half strength of MS medium containing 0.2 mg/l IBA. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully established in the field.Bangladesh J. Sci. Ind. Res.53(2), 133-138, 2018

Evaluation of the Enhanced Regeneration System for in vitro regeneration in barley

2006 ◽  
Vol 86 (1) ◽  
pp. 63-69
Author(s):  
Seedhabadee Ganeshan ◽  
Brian J Weir ◽  
Monica Båga ◽  
Brian G Rossnagel ◽  
Ravindra N Chibbar
Keyword(s):  
Hordeum Vulgare ◽  
Embryogenic Callus ◽  
Culture Media ◽  
In Vitro Regeneration ◽  
Cold Treatment ◽  
Regeneration System ◽  
Embryogenic Calli ◽  
Hordeum Vulgare L ◽  
Best Response

A simple two-step model for evaluation of in vitro regeneration protocols is proposed based on callus induction and regeneration from immature scutella of two Canadian barley (Hordeum vulgare L.) genotypes, AC Metcalfe and SB92559 using the Enhanced Regeneration System (ERS). The number of explants producing embryogenic callus, the number of plants per embryogenic callus and the number of plants per explant were considered. Tissue culture parameters included three combinations of growth regulators, two carbon sources in culture media, and three cold treatment regimes of spikes prior to scutella isolation. Culture medium containing 5 µM 2,4-dichlorophenoxy acetic acid (2,4-D) and 0.5 µM benzyl adenine (BA) induced the highest percent of embryogenic calli and the highest number of shoots per embryogenic callus from AC Metcalfe. Medium containing 3.75 µM 2,4-D and 0.75 µM BA gave the best response for SB92559. Both genotypes produced more shoots on maltose than on sucrose medium. A 2-d treatment of spikes at 4°C resulted in best response for SB92559. Regeneration response from AC Metcalfe scutella from spikes was unaffected by being subjected to 2, 4 or 6 d of cold. Conditions resulting in best responses from both genotypes were tested on four commercial barley varieties. However, these lines showed inferior regeneration compared to SB92559 and AC Metcalfe. Key words: Hordeum vulgare, scutella, embryogenic callus, shoot production

Effective and simple in vitro regeneration system of Miscanthus sinensis , M . × giganteus and M . sacchariflorus for planting and biotechnology purposes

2017 ◽  
Vol 107 ◽  
pp. 219-226 ◽  
Author(s):  
Aurelia Ślusarkiewicz-Jarzina ◽  
Aleksandra Ponitka ◽  
Joanna Cerazy-Waliszewska ◽  
Maria Katarzyna Wojciechowicz ◽  
Karolina Sobańska ◽  
...  
Keyword(s):  
In Vitro Regeneration ◽  
Miscanthus Sinensis ◽  
Regeneration System

An efficient in vitro regeneration system for Lythrum salicaria

2009 ◽  
Vol 53 (4) ◽  
pp. 750-754 ◽  
Author(s):  
A. U. Turker ◽  
B. Yucesan ◽  
E. Gurel
Keyword(s):  
In Vitro Regeneration ◽  
Lythrum Salicaria ◽  
Regeneration System

scholarly journals 117 An In Vitro Regeneration System for Basil (Ocimum basilicum L.)

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 461E-461
Author(s):  
Winthrop B. Phippen ◽  
James E. Simon
Keyword(s):  
In Vitro Regeneration ◽  
Morphological Characteristics ◽  
Leaf Explants ◽  
Basal Medium ◽  
Induction Medium ◽  
Shoot Induction ◽  
Regeneration System ◽  
Transformation Protocol ◽  
Regeneration Procedure

A plant regeneration protocol was successfully developed for basil (O. basilicum L.). Explants from 1-month-old seedlings yielded the highest frequency of regeneration of shoots (37%) with an average number of 3.6 shoots per explant. Calli and shoot induction were initiated on Murashige and Skoog (MS) basal medium supplemented with thidiazuron (TDZ) (4 mg/L) for ≈30 days. Shoot induction and development was achieved by refreshing the induction medium once after 14 days. The most morphogenetically responsive explants were basal leaf explants from the first fully expanded true leafs of greenhouse-grown basil seedlings. Developing shoots were then rooted on MS media in the dark without TDZ. Within 20 days, rooted plantlets were transferred and acclimatized under greenhouse conditions where they developed normal morphological characteristics. This is the first report of a successful in vitro regeneration system for basil through primary callus. The establishment of a reliable regeneration procedure is critical when developing a transformation protocol for enhancing the production of basil for insect and disease resistance and improved essential oil constituents.

scholarly journals Avaliação da capacidade de produzir fitotoxinas in vitro por parte de fungos com propriedades antagônicas a nematóides

2006 ◽  
Vol 30 (6) ◽  
pp. 1230-1235
Author(s):  
Daniel Diego Costa Carvalho ◽  
Denilson Ferreira de Oliveira ◽  
Vicente Paulo Campos ◽  
Moacir Pasqual ◽  
Renato Mendes Gimarães ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Lactuca Sativa ◽  
Fusarium Moniliforme ◽  
Triticum Aestivum L ◽  
Paecilomyces Lilacinus ◽  
Penicillium Sp ◽  
Lactuca Sativa L

Em trabalho preliminar foram obtidos 24 isolados fúngicos com propriedades antagônicas a nematóides parasitas de plantas segundo experimentos realizados em laboratório. Para dar continuidade a tal trabalho, tornou-se desejável dispor de metodologia simples e de baixo custo para selecionar aqueles sem capacidade de produção de substâncias fitotóxicas, para serem empregados em experimentos em casa-de-vegetação com fitonematóides. Conseqüentemente, cultivaram-se os fungos em meio líquido e, após filtração, as fases líquidas foram liofilizadas e extraídas com acetato de etila/metanol. Os extratos foram concentrados sob vácuo e dissolvidos em água e solução de sacarose para serem submetidos a testes in vitro com sementes de alface (Lactuca sativa L.) e com coleóptilos de trigo (Triticum aestivum L.), respectivamente. Vinte e três isolados pertencentes aos gêneros Arthrobotrys, Aspergillus, Coniothyrium, Cunninghamella, Cylindrocarpon, Fusarium, Monacrosporium, Mortierella, Paecilomyces, Penicillium, Sclerotinia, Trichoderma e Verticillium, apresentaram atividade tóxica contra os coleóptilos de trigo. Apenas os isolados de Cylindrocarpon magnusianum (Sacc.) Wollenw., Fusarium moniliforme Shelden, Mortierella sp., Paecilomyces lilacinus (Thom.) Samson e Penicillium sp. apresentaram atividade fitotóxica no teste com sementes de alface. Somente um isolado de F. moniliforme se mostrou inativo em ambos os testes, o que sugere que não seja produtor de fitotoxinas.

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