In vitro regeneration, antioxidant potential, and genetic fidelity analysis of Asystasia gangetica (L.) T.Anderson

The present study reports a simple protocol for in vitro regeneration of Aglaonema ‘Valentine’ using axillary shoot explants for rapid multiplication and production of true-to-type plants. Different concentrations of benzyladenine (BA; 0, 1, 3, 5, and 7 mg·L−1), kinetin (Kin; 0, 1, 3, 5, and 7 mg·L−1), thidiazuron (TDZ; 0, 0.5, 1.0, 1.5, and 2.0 mg·L−1), naphthalene acetic acid (NAA; 0, 0.5, and 1.0 mg·L−1), and indole-3-butyric acid (IBA; 0, 0.5, and 1.0 mg·L−1) were used for shoot regeneration. The highest shoot proliferation (5.0) was obtained on Murashige and Skoog (MS) medium supplemented with 1.5 mg·L−1 TDZ and 1 mg·L−1 NAA. In vitro rooting was easily achieved with 100% at all concentrations of NAA and IBA supplemented to half- or full-strength MS medium. Regenerated plantlets were acclimatized in greenhouse with 100% survival rate. Randomly amplified polymorphic DNA (RAPD) analysis confirmed the genetic fidelity of the regenerated plantlets and mother plant.

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In vitro regeneration and assessment of genetic fidelity of acclimated plantlets by using ISSR markers in PPR-1 (Morus sp.): An economically important plant

Scientia Horticulturae ◽

10.1016/j.scienta.2018.07.012 ◽

2018 ◽

Vol 241 ◽

pp. 313-321 ◽

Cited By ~ 16

Author(s):

Gulab Khan Rohela ◽

Phanikanth Jogam ◽

Aftab Ahmad Shabnam ◽

Pawan Shukla ◽

Sadanandam Abbagani ◽

...

Keyword(s):

In Vitro Regeneration ◽

Genetic Fidelity ◽

Issr Markers

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The influential role of polyamines on the in vitro regeneration of pea (Pisum sativum L.) and genetic fidelity assessment by SCoT and RAPD markers

Plant Cell Tissue and Organ Culture (PCTOC) ◽

10.1007/s11240-019-01699-z ◽

2019 ◽

Vol 139 (3) ◽

pp. 547-561 ◽

Cited By ~ 2

Author(s):

Chandrasekaran Ajithan ◽

Venkatachalam Vasudevan ◽

Dorairaj Sathish ◽

Selvam Sathish ◽

Veda Krishnan ◽

...

Keyword(s):

Pisum Sativum ◽

Rapd Markers ◽

In Vitro Regeneration ◽

Genetic Fidelity ◽

Pisum Sativum L ◽

Fidelity Assessment

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Do magnetic field applications affect in vitro regeneration, growth, phenolic profiles, antioxidant potential and defense enzyme activities (SOD, CAT and PAL) in lemon balm (Melissa officinalis L.)?

Industrial Crops and Products ◽

10.1016/j.indcrop.2021.113624 ◽

2021 ◽

Vol 169 ◽

pp. 113624

Author(s):

Canan Ulgen ◽

Arzu Birinci Yildirim ◽

Günce Sahin ◽

Arzu Ucar Turker

Keyword(s):

Magnetic Field ◽

Enzyme Activities ◽

In Vitro Regeneration ◽

Antioxidant Potential ◽

Melissa Officinalis ◽

Lemon Balm ◽

Defense Enzyme ◽

Phenolic Profiles

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In Vitro Regeneration and ISSR-Based Genetic Fidelity Analysis of Orthosiphon stamineus Benth

Agronomy ◽

10.3390/agronomy9120778 ◽

2019 ◽

Vol 9 (12) ◽

pp. 778 ◽

Cited By ~ 2

Author(s):

Hanisah Ali ◽

Izzah Farhanah Musa ◽

Nurul Atikhah Abu Bakar ◽

Saiful Anuar Karsani ◽

Jamilah Syafawati Yaacob

Keyword(s):

In Vitro Regeneration ◽

Genetic Fidelity ◽

Epileptic Seizures ◽

Black Soil ◽

Inter Simple Sequence Repeat ◽

Issr Markers ◽

Direct Regeneration ◽

Nodal Segment ◽

Orthosiphon Stamineus

Orthosiphon stamineus has been widely used as traditional remedy for various illnesses and diseases, such as cardiovascular diseases and epileptic seizures. In this study, direct regeneration through nodal segment of this species was attempted using Kinetin (6-Furfurylaminopurine) and IAA (indole-3-acetic acid). Optimum regeneration media was identified as MS media supplemented with 2.0 mg L−1 Kin plus 0.5 mg L−1 IAA. This yielded the highest number of shoots (5.57 ± 0.42) and leaves (20.53 ± 1.91) per explant. Acclimatization of the resulting in vitro regenerants was successful in all potting mixtures tested. However, potting mixture PF (1:1:1 ratio of black soil/red soil/compost) was identified as the best medium for acclimatization of this species, as it yielded 100% survival percentage after 90 days of acclimatization. Ten in vitro regenerants of O. stamineus were randomly collected after the third subculture and subjected to genetic variation analysis using inter-simple sequence repeat (ISSR) markers. Out of 20 ISSR markers tested, 10 working primers were observed to produce satisfactory amplification of bands, with an average of 7.11 bands per primer. A total of 610 bands were produced by the 10 primers. The percentage of polymorphism was observed to be very low, yielding only 7.32% polymorphism among all samples. Jaccard dissimilarity analysis was also conducted and very low genetic distance (about 0.1) was found among the in vitro regenerants and between the regenerants with the mother plant, thus ascertaining the clonal nature of the plantlets produced in this study.

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