First report of Verticillium dahliae causing Verticillium wilt on kiwifruit in Ordu, Turkey

2019 ◽  
Vol 102 (1) ◽  
pp. 221-222 ◽  
Author(s):  
Muharrem Türkkan ◽  
Nusret Şahin ◽  
Göksel Özer ◽  
Zeynep Evgin ◽  
Mehmet Yaman ◽  
...  
Keyword(s):  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
First Report

scholarly journals First Report of Verticillium Wilt Caused by Verticillium dahliae Impacting Ailanthus altissima in Virginia, U.S.A.

Plant Disease ◽  
2020 ◽  
Vol 104 (5) ◽  
pp. 1558 ◽  
Author(s):  
R. K. Brooks ◽  
A. L. Snyder ◽  
E. A. Bush ◽  
S. M. Salom ◽  
A. Baudoin
Keyword(s):  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Ailanthus Altissima ◽  
First Report

scholarly journals First report of Verticillium dahliae causing verticillium wilt on Stevia rebaudiana in Europe

2019 ◽  
Vol 101 (4) ◽  
pp. 1291-1291 ◽  
Author(s):  
Daniele Da Lio ◽  
Luigi De Martino ◽  
Silvia Tavarini ◽  
Barbara Passera ◽  
Luciana Gabriella Angelini ◽  
...  
Keyword(s):  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Stevia Rebaudiana ◽  
First Report

scholarly journals Verticillium Wilt of Tilia japonica and Acer palmatum, the First Report on the Occurrence of Verticillium dahliae on Trees in Japan.

1997 ◽  
Vol 63 (4) ◽  
pp. 345-350 ◽  
Author(s):  
Yukio HARADA ◽  
Tomoya FURUEDA ◽  
Kazutoshi MURATA
Keyword(s):  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
First Report

scholarly journals First report of Verticillium wilt on cultivated radish caused by Verticillium dahliae Kleb. in China

2019 ◽  
Vol 101 (3) ◽  
pp. 777-777
Author(s):  
Wenxue Yan ◽  
Baoju Li ◽  
Ali Chai ◽  
Yanxia Shi ◽  
Jianjun Shi ◽  
...  
Keyword(s):  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
First Report ◽  
Verticillium Dahliae Kleb

scholarly journals First Report of Verticillium Wilt Caused by Verticillium dahliae on Mango Trees (Mangifera indica) in Southern Spain

Plant Disease ◽  
2010 ◽  
Vol 94 (3) ◽  
pp. 380-380 ◽  
Author(s):  
L. Baeza-Montañez ◽  
R. Gómez-Cabrera ◽  
M. D. García-Pedrajas
Keyword(s):  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Mangifera Indica ◽  
Southern Spain ◽  
Reference Sequence ◽  
Universal Primers ◽  
First Report ◽  
Wide Range ◽  
Race 1 ◽  
Disease Rating

Verticillium wilt, primarily caused by Verticillium dahliae Klebahn and V. albo-atrum Reinke & Berthold, affects a wide range of economically important crops. This disease is an increasing problem in areas where young mango trees are planted on land previously planted in vegetable crops. In 2008, symptoms of Verticillium wilt were observed in mango cvs. Kent and Osteen in the subtropical fruit-producing area of Málaga in southern Spain. In a new mango grove of cv. Kent, previously planted in potatoes and tomatoes, ~20% of 200 1-year-old trees had one-sided branch dieback. In many of these trees the symptoms expanded, leading to decline and eventual death. Cross sections of affected branches revealed brown vascular discoloration. Verticillium was isolated from surface-sterilized segments of symptomatic branches placed on acidic potato dextrose agar (PDA). Plates were incubated at 24°C. After 3 days, slow-growing colonies were transferred to PDA. Verticillium was similarly isolated from symptomatic potato plants grown in a nearby field. Identification of V. dahliae was initially based on morphology and further confirmed by molecular methods. All isolates tested produced microsclerotia, a defining feature that distinguishes V. dahliae from V. albo-atrum. For molecular characterization, V. dahliae specific primers 19 and 22 (1) and universal primers ITS1 and ITS4, which amplify the rRNA internal transcribed spacer (ITS) region (4), were used. Bands of expected size were amplified with both primer combinations. ITS fragments were sequenced and identical to the V. dahliae reference sequence (GenBank AY555948) (3). Pathogenicity assays were conducted with a selected isolate from mango using tomato plants from the susceptible line ‘Moneymaker’ and the near isogenic ‘Motabo’ line carrying the Ve gene conferring resistance to race 1 isolates. Five 1-month-old plants (four-leaf stage) were inoculated by root immersion in a suspension of 107 conidia/ml. Five control plants were mock inoculated with distilled water. As a positive control, five plants were inoculated with the previously described race 1 strain Dvd-T5 (2), which induces severe symptoms in susceptible tomato cultivars. Symptoms were scored visually at various time points up to 40 days by a 0 to 5 scale in which 0 = negligible chlorosis or wilting, 1 = chlorosis and wilting and/or curling in individual leaves, 2 = necrosis in leaves, 3 = at least one branch dead, 4 = wilt and/or chlorosis in upper leaves and/or two or more branches dead, and 5 = plant dead or all leaves and most of stem necrotic. The isolate from mango caused typical Verticillium wilt symptoms with a mean disease rating of 3.6 at 40 days postinoculation in both lines. The mean disease rating for Dvd-T5 in Moneymaker 40 days postinoculation was 4.0. V. dahliae was reisolated from symptomatic plants but not from noninoculated controls. To our knowledge, this is the first report of Verticillium wilt on mango in Spain. More problems with Verticillium wilt are expected because of the increasing planting of mango in fields previously dedicated to horticultural crops. References: (1) J. H. Carder et al. Modern Assays for Plant Pathogenic Fungi: Identification, Detection and Quantification. CAB International, Oxford, 1994. (2) K. F. Dobinson et al. Can. J. Plant. Pathol. 18:55, 1996. (3) M. P. Pantou et al. Mycol. Res. 109:889, 2005. (4) T. J. White et al. PCR Protocols: A Guide to Methods and Amplification. Academic Press, San Diego, 1990.

scholarly journals First Report of Verticillium dahliae Causing Artichoke Wilt in California

Plant Disease ◽  
1999 ◽  
Vol 83 (8) ◽  
pp. 782-782 ◽  
Author(s):  
R. G. Bhat ◽  
K. V. Subbarao ◽  
M. A. Bari
Keyword(s):  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Infected Plant ◽  
Potato Dextrose Agar ◽  
The Other ◽  
Central Coast ◽  
Cynara Scolymus ◽  
First Report ◽  
Vascular Discoloration ◽  
Infested Field

In mid-August 1998, artichoke (Cynara scolymus L.) plants of cultivar Imperial Star in a field in the Salinas area of the central coast of California developed wilt symptoms. The plants were stunted with chlorotic, drooping, and dried leaves near the bottom and middle of the plants as previously described in Italy (1). Diseased plants produced smaller edible buds and, in severe cases, buds were discolored with dried outer bracts. Roots exhibited the characteristic vascular discoloration of Verticillium infection. In one part of the infested field, artichoke was near harvest with 85% of plants showing wilt symptoms with vascular discoloration whereas the other part had a 60-day-old crop with 98% of plants infected. Yield in the field was reduced by as much as 50%. Verticillium dahliae was isolated from infected plant samples on NP-10 medium (2), and isolates were single spored before storing on potato dextrose agar at 4°C. Identity of the pathogen was confirmed based on colony morphology and formation of microsclerotia. In root-dip inoculation tests in the greenhouse, two V. dahliae isolates from artichoke infected 1-month-old artichoke seedlings that wilted within 6 weeks of inoculation. V. dahliae was reisolated from plants showing vascular discoloration. Cross-inoculation studies revealed that artichoke isolates caused a moderate level of disease in lettuce, but only a trace of vascular discoloration in cauliflower. Lettuce isolates caused a severe wilt in artichoke. Cauliflower isolates did not cause wilt in lettuce and caused only slight vascular discoloration in artichoke. This is the first report of a Verticillium wilt of artichoke in California. References: (1) M. Cirulli et al. Plant Dis. 78:680, 1994. (2) L. H. Sorensen et al. Phytopathology 81:1347, 1991.

scholarly journals First Report of Verticillium dahliae Causing Verticillium Wilt on Potato in Korea

Plant Disease ◽  
2019 ◽  
Vol 103 (9) ◽  
pp. 2470-2470
Author(s):  
J.-S. Kim ◽  
Y. K. Lee ◽  
S. K. Hong ◽  
H.-W. Choi
Keyword(s):  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
First Report

scholarly journals First Report of Verticillium Wilt caused by Verticillium dahliae Kleb. on New Zealand Spinach (Tetragonia tetragonioides) in Italy

Plant Disease ◽  
2013 ◽  
Vol 97 (1) ◽  
pp. 145-145
Author(s):  
A. Garibaldi ◽  
S. Rapetti ◽  
P. Martini ◽  
L. Repetto ◽  
D. Bertetti ◽  
...  
Keyword(s):  
New Zealand ◽  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
Vascular Tissue ◽  
Spinacia Oleracea ◽  
Morphological Characteristics ◽  
Conidial Suspension ◽  
Potato Dextrose Broth ◽  
First Report ◽  
Tetragonia Tetragonioides

Tetragonia tetragonioides (New Zealand spinach, Aizoaceae) is an Australasian annual species that occurs naturally in Italy, where it is cultivated for the edible young shoots and succulent leaves. In September 2011, a previously unknown wilt was observed in 10 private gardens, each 0.1 to 0.5 ha, near Castellaro, Northern Italy, on 7-month-old New Zealand spinach plants. Leaves wilted, starting from the collar and moving up the plant, and vascular tissues showed brown streaks in the roots, crowns, and stems. Diseased plants were stunted with small, chlorotic leaves. Infected stems and leaves then wilted, and plants often died. Of about 500 plants, 30% were affected. Stems of 10 diseased plants were disinfected with 1% NaOCl for 1 min. Sections of symptomatic vascular tissue were plated on potato dextrose agar. After 3 days at 23 ± 1°C, colonies developed that were white and turned a grey to dark green color. Irregular, black microsclerotia (32.0) 63.1 ± 16.8 μm (106.1) × (18.7) 39.1 ± 12.3 μm (65.8) developed in hyaline hyphae after 8 days. Hyaline, elliptical, single-celled conidia (2.7) 3.8 ± 0.6 μm (4.8) × (1.9) 2.6 ± 0.5 μm (3.5) developed on verticillate conidiophores with three phialides at each node. Based on these morphological characteristics, the fungus was identified as Verticillium dahliae (1). The internal transcribed spacer (ITS) region of rDNA was amplified for one isolate using the primers ITS1/ITS4 (3) and sequenced (GenBank Accession No. JX308315). BLASTn analysis of the 479-bp segment showed 100% homology with the ITS sequence of a V. dahliae isolate (AB551206). Pathogenicity tests were performed twice using 60-day-old plants of T. tetragonioides. Unwounded roots of eight plants were dipped for 1 min in a conidial suspension (5 × 107 conidia/ml) of one isolate of V. dahliae obtained from the original infected New Zealand spinach plants, and grown in potato dextrose broth. The inoculated plants were transplanted into 2-liter pots (1 plant/pot) containing steamed potting mix (sphagnum peat-perlite-pine bark-clay; 50:20:20:10) and maintained in a growth chamber at 20 to 24°C and 50 to 80% RH. Eight plants immersed in sterile water served as a control treatment. Wilt symptoms were observed 30 days after inoculation, with vascular discoloration in the roots, crowns and stems. V. dahliae was reisolated consistently from infected tissues, but not from the control plants that remained healthy. Pathogenicity was also tested using the same method on plants of four cultivars (five plants/cultivar) of Spinacia oleracea (Matador, Asti, Merlo Nero, and America). Wilt symptoms developed on all cultivars and V. dahliae was reisolated from each inoculated plant. No fungal colonies were reisolated from control plants, which remained healthy. To our knowledge, this is the first report of Verticillium wilt caused by V. dahliae on T. tetragonioides in Italy, as well in Europe. V. dahliae was reported on T. tetragonioides in Canada (2). At this time, the economic impact of Verticillium wilt on New Zealand Spinach in Italy is limited, although the use of this vegetable in Italy is increasing. References: (1) G. F. Pegg and B. L. Brady. Verticillium Wilts. CABI Publishing, Wallingford, UK, 2002. (2) M. J. Richardson. Page 387 in: An Annotated List of Seed-Borne Diseases, Fourth Edition. International Seed Testing Association, Zurich, Switzerland, 1990. (3) T. J. White et al. Page 315 in: PCR Protocols. A Guide to Methods and Applications. Academic Press, San Diego, CA, 1990.

scholarly journals First report of verticillium wilt of artichoke caused by Verticillium dahliae in Tunisia

2008 ◽  
Vol 57 (2) ◽  
pp. 377-377 ◽  
Author(s):  
H. Jabnoun-Khiareddine ◽  
M. Daami-Remadi ◽  
F. Ayed ◽  
M. El Mahjoub
Keyword(s):  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
First Report

First report of verticillium wilt of melon caused by Verticillium dahliae in Tunisia

2007 ◽  
Vol 56 (4) ◽  
pp. 726-726 ◽  
Author(s):  
H. Jabnoun-Khiareddine ◽  
M. Daami-Remadi ◽  
F. Ayed ◽  
M. El Mahjoub
Keyword(s):  
Verticillium Wilt ◽  
Verticillium Dahliae ◽  
First Report
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