Host plant resistance profiling of chickpea genotypes against Helicoverpa armigera (Hübner) through SEM and GC–MS studies

2022 ◽  
Author(s):  
M. S. Sai Reddy ◽  
Meena Agnihotri ◽  
S. D. Divija ◽  
Babita Belal ◽  
Somala Karthik
Keyword(s):  
Host Plant ◽  
Helicoverpa Armigera ◽  
Plant Resistance ◽  
Host Plant Resistance ◽  
Helicoverpa Armígera ◽  
Helicoverpa Armigera Hubner

scholarly journals Comparative TMT Proteomic Analysis Unveils Unique Insights into Helicoverpa armigera (Hübner) Resistance in Cajanus scarabaeoides (L.) Thouars

2021 ◽  
Vol 22 (11) ◽  
pp. 5941
Author(s):  
Abigail Ngugi-Dawit ◽  
Isaac Njaci ◽  
Thomas J.V. Higgins ◽  
Brett Williams ◽  
Sita R. Ghimire ◽  
...  
Keyword(s):  
Host Plant ◽  
Helicoverpa Armigera ◽  
Plant Resistance ◽  
Host Plant Resistance ◽  
Proteomic Analysis ◽  
Artificial Diet ◽  
Growth Stages ◽  
Genetic Base ◽  
Helicoverpa Armígera ◽  
Helicoverpa Armigera Hubner

Pigeonpea [Cajanus cajan (L.) Millspaugh] is an economically important legume playing a crucial role in the semi-arid tropics. Pigeonpea is susceptible to Helicoverpa armigera (Hübner), which causes devastating yield losses. This pest is developing resistance to many commercially available insecticides. Therefore, crop wild relatives of pigeonpea, are being considered as potential sources of genes to expand the genetic base of cultivated pigeonpea to improve traits such as host plant resistance to pests and pathogens. Quantitative proteomic analysis was conducted using the tandem mass tag platform to identify differentially abundant proteins between IBS 3471 and ICPL 87 tolerant accession and susceptible variety to H. armigera, respectively. Leaf proteome were analysed at the vegetative and flowering/podding growth stages. H. armigera tolerance in IBS 3471 appeared to be related to enhanced defence responses, such as changes in secondary metabolite precursors, antioxidants, and the phenylpropanoid pathway. The development of larvae fed on an artificial diet with IBS 3471 lyophilised leaves showed similar inhibition with those fed on an artificial diet with quercetin concentrations with 32 mg/25 g of artificial diet. DAB staining (3,3’-diaminobenzidine) revealed a rapid accumulation of reactive oxygen species in IBS 3471. We conclude that IBS 3471 is an ideal candidate for improving the genetic base of cultivated pigeonpea, including traits for host plant resistance.

​Screening of Pigeonpea Varieties through Nylon Bag No-choice Bioassay for Host Plant Resistance to Helicoverpa armigera

2021 ◽  
Author(s):  
B.L. Jat ◽  
K.K. Dahiya ◽  
H.C. Sharma
Keyword(s):  
Host Plant ◽  
Helicoverpa Armigera ◽  
Plant Resistance ◽  
Host Plant Resistance ◽  
Adult Emergence ◽  
Larval Survival ◽  
Trichome Density ◽  
Larval Weight ◽  
Legume Pod Borer ◽  
Helicoverpa Armígera

Background: The legume pod borer, Helicoverpa armigera (Hübner), is one of the most damaging crop pests, including pigeonpea. Host plant resistance is a component of pest management and therefore, we standardize a nylon bag No-Choice Bioassay technique to screen for resistance to H. armigera under field conditions. Methods: Pigeonpea plants were infested with 24 h old 1, 2, 3, 4 and 5 larvae per plant inside the nylon bag. Observations were recorded on pod damage, larval survival, larval weight, pupation, adult emergence, and fecundity after 10 days. Result: Pigeonpea varieties AL-201, H03-41 and PAU-881 exhibited lower pod damage (15.89 to 19.77%) and larval weight (12.02 to 13.82 mg). The expression of resistance to H. armigera was associated with trichome density, pod wall thickness and higher amount of phenolic compounds and condensed tannins. Lower trichome density and thin pod walls and higher amounts of sugars rendered the varieties Paras, Manak and Pussa-992 more susceptible to H. armigera. Nylon bag assay can be used to screen and select pigeonpea cultivars for resistance to H. armigera.

scholarly journals Host Plant Resistance to Helicoverpa armigera Has Been Observed in Australian Wild Cajanus (Pigeonpea) Species

Proceedings ◽  
2020 ◽  
Vol 36 (1) ◽  
pp. 149
Author(s):  
Vanambathina Prameela Rani
Keyword(s):  
Host Plant ◽  
Helicoverpa Armigera ◽  
Plant Resistance ◽  
Host Plant Resistance ◽  
Wild Species ◽  
Insect Pest ◽  
Significant Negative Correlation ◽  
Total Phenolic ◽  
Resistant Species ◽  
Helicoverpa Armígera

: Pigeonpea (Cajanus cajan (L) Millspaugh) is extensively grown in tropical subtropical and warmer equatorial regions of the world and ranks 6th in the global legume production (6.8MT; 2017). It is consumed by billions of people as a major source of protein in developing countries. Despite the increasing demand the crop is facing severe yield losses (2.4 billion US$/Annum) due to the insect pest Helicoverpa.armigera (68% globally). As this pest is polyphagous was exposed to several pesticides and gained strong resistance, it necessities a strong need for identifying host plant resistance towards this pest. Australia is the second hub with 15 out of 32 Cajanus species next to Asia. This is the pilot study exploring the potential of Australian Cajanus species as wild were proven sources for potential trait information in many other crops. My study firstly characterized the Australasian wild species and their derivatives for phenotypic variation and secondly tested for their resistance to Helicoverpa armigera. My research also demonstrated a very high levels of resistance to Helicoverpa armigera in some of the Australian wild species and their derivatives, compared to cultivated species. This research also revealed a high levels of total phenolic compounds in the wild species and a significant negative correlation of total phenols with the pest survival. We were successful in identifying the major phenolic compound in resistant species. Exploring the role of that specific compound related to host plant resistance and identifying the genetic markers associated with phytochemicals triggering the pest resistance is underway.

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