Abortion of early pregnancy by the intravaginal administration of prostaglandin F2α

1973 ◽  
Vol 117 (2) ◽  
pp. 246-250 ◽  
Author(s):  
Ronald J. Bolognese ◽  
Stephen L. Corson
Keyword(s):  
Early Pregnancy ◽  
Prostaglandin F2α ◽  
Intravaginal Administration

Oxytocin-induced prostaglandin F2-alpha release is low in early bovine pregnancy but increases during the second month of pregnancy†

2019 ◽  
Author(s):  
Jéssica N Drum ◽  
Milo C Wiltbank ◽  
Pedro L J Monteiro ◽  
Alexandre B Prata ◽  
Rodrigo S Gennari ◽  
...  
Keyword(s):  
Corpus Luteum ◽  
Early Pregnancy ◽  
Artificial Insemination ◽  
Peripheral Blood ◽  
Prostaglandin F2α ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Interferon Stimulated Genes ◽  
Prostaglandin F2 Alpha ◽  
Oxytocin Receptors

Abstract Circulating prostaglandin F2α metabolite (PGFM) after an oxytocin challenge was evaluated throughout the first 2 months of pregnancy in lactating Holstein cows. On day 11, 18, and 25 after artificial insemination (AI), and on days 32, 39, 46, 53, and 60 of pregnancy, cows were challenged with 50 IU oxytocin, i.m. Blood was collected before (0 min), 30, 60, 90, and 120 min after oxytocin for plasma PGFM concentrations. Ultrasound evaluations were performed for pregnancy diagnosis on day 32–60 post-AI. Nonpregnant (NP) cows on day 18 were designated by a lack of interferon-stimulated genes in peripheral blood leukocytes and Pregnant (P) based on day 32 ultrasound. On day 11, P and NP were similar with low PGFM and no effect of oxytocin on PGFM. On day 18, oxytocin increased PGFM (3-fold) in NP with little change in P cows. Comparing only P cows from day 11 to 60, basal circulating PGFM increased as pregnancy progressed, with day 11 and 18, lower than all days from day 25 to 60 of pregnancy. Oxytocin-induced PGFM in P cows on day 25 was greater than P cows on day 18 (2.9-fold). However, oxytocin-induced PGFM was lower on day 25 compared to day 53 and 60, with intermediate values on day 32, 39, and 46 of pregnancy. Thus, the corpus luteum (CL) of early pregnancy (day 11, 18) is maintained by suppression of PGF, as reflected by suppressed PGFM in this study. However, during the second month of pregnancy, uterine PGF secretion was not suppressed since basal PGFM and oxytocin-induced PGFM secretion were elevated. Apparently, mechanisms other than suppression of oxytocin receptors maintain CL after day 25 of pregnancy.

Levels of prostaglandin F2α metabolites in blood and urine during early pregnancy

1984 ◽  
Vol 7 (1-3) ◽  
pp. 133-148 ◽  
Author(s):  
H. Kindahl ◽  
S. Basu ◽  
G. Fredriksson ◽  
A. Goff ◽  
A. Kunavongkrit ◽  
...  
Keyword(s):  
Early Pregnancy ◽  
Prostaglandin F2α

Intrauterine injection of 15(S)-15-methyl-prostaglandin F2α for termination of early pregnancy in out-patient

1975 ◽  
Vol 10 (2) ◽  
pp. 333-341 ◽  
Author(s):  
O. Ylikorkala ◽  
P. Kirkinen ◽  
P. Jouppila ◽  
P.A. Järvinen
Keyword(s):  
Early Pregnancy ◽  
Prostaglandin F2α

PROGESTERONE, PROSTAGLANDIN F2α, AND OESTRADIOL IN THE UTERO-OVARIAN VENOUS PLASMA OF NON-PREGNANT AND EARLY, UNILATERALLY PREGNANT GUINEA-PIGS

1975 ◽  
Vol 67 (2) ◽  
pp. 225-229 ◽  
Author(s):  
F. R. BLATCHLEY ◽  
F. M. MAULE WALKER ◽  
N. L. POYSER
Keyword(s):  
Guinea Pig ◽  
Early Pregnancy ◽  
Guinea Pigs ◽  
Prostaglandin F2α ◽  
Progesterone Secretion ◽  
Prostaglandin F ◽  
Venous Plasma

SUMMARY Levels of progesterone, prostaglandin F2α (PGF2α) and oestradiol in the utero-ovarian venous plasma of non-pregnant and early, unilaterally pregnant guinea-pigs were compared. Progesterone levels fell from day 12 to day 15 in the non-pregnant animals, while levels of PGF2α and oestradiol increased during this period. In contrast, in the pregnant guinea-pigs, progesterone levels remained high up to day 15 and PGF2α and oestradiol levels remained low in plasma from both the pregnant and non-pregnant sides. These findings support the hypothesis that the guinea-pig conceptus secretes an anti-luteolytic factor in early pregnancy, which reduces the amount of PGF2α produced by the uterus and released into the uterine veins and thereby allows luteal maintenance and continued progesterone secretion. Oestradiol, in the presence of progesterone, is regarded as the physiological stimulus for normal uterine PGF2α synthesis and release. Consequently, the inhibition of ovarian oestradiol secretion seen in early pregnancy is probably at least part of the mechanism by which the anti-luteolytic factor produced by the conceptus reduces PGF2α synthesis by and release from the uterus.

Effect of Prostaglandin F2α Administration on Early Pregnancy in Ewes

1987 ◽  
Vol 64 (4) ◽  
pp. 1127-1133 ◽  
Author(s):  
P. E. Naasz ◽  
A. L. Slyter
Keyword(s):  
Early Pregnancy ◽  
Prostaglandin F2α

Oxytocin-stimulated phosphoinositide hydrolysis and prostaglandin F2α secretion by luminal epithelial, glandular epithelial and stromal cells from pig endometrium. II. Responses of cyclic, pregnant and pseudopregnant pigs on Days 12 and 1

2000 ◽  
Vol 12 (4) ◽  
pp. 157 ◽  
Author(s):  
Mehmet Uzumcu ◽  
Kevin G. Carnahan ◽  
Gheorghe T. Braileanu ◽  
Mark A. Mirando
Keyword(s):  
Epithelial Cells ◽  
Early Pregnancy ◽  
Stromal Cells ◽  
Prostaglandin F2α ◽  
Secretory Response ◽  
Reproductive Status ◽  
Phosphoinositide Hydrolysis ◽  
Pregnancy Recognition ◽  
Luminal Epithelial Cells

In pigs, the exact mechanism for the shift in endometrial PGF 2α secretion from an endocrine to an exocrine mode during pregnancy recognition is not known. The objective of this study was to examine whether this shift involved a change in the responsiveness of luminal epithelial, glandular epithelial and stromal cells to 0 or 100 nM oxytocin. Luminal epithelial cells, glandular epithelial cells and stromal cells were isolated from cyclic, pregnant or oestrogen-induced pseudopregnant gilts on Day 12 (Experiment 1) or Day 16 (Experiment 2) post oestrus (oestrus = Day 0). For cells obtained on Day 12, oxytocin stimulated PGF2α secretion by stromal cells (P<0.01) similarly for each reproductive status, whereas oxytocin stimulated PGF 2α secretion from luminal and glandular epithelial cells (P<0.05) from pregnant and pseudopregnant gilts but not from cyclic gilts. For both concentrations of oxytocin, mean PGF2α secretion was less (P<0.05) from stromal cells of pregnant than cyclic gilts. For cells obtained on Day 16, oxytocin stimulated PGF 2α release from stromal cells of cyclic gilts but not from stromal cells of pregnant gilts. Mean PGF 2α secretion also was less (P<0.05) from stromal cells of pregnant gilts than cyclic gilts. Oxytocin tended to stimulate PGF 2α release (P<0.07) from glandular epithelial cells of cyclic but not pregnant or pseudopregnant gilts. Luminal epithelial cells from all reproductive statuses were similarly unresponsive to oxytocin. In conclusion, the increased PGF2α secretory response to oxytocin of luminal and glandular epithelial cells from pregnant gilts on Day 12, combined with the decreased response of stromal cells from pregnant gilts on Days 12 and 16, may contribute, in part, to the shift in endometrial PGF2α secretion from an endocrine to an exocrine direction during early pregnancy in pigs.

34 COMPARISON OF IN VIVO DEVELOPMENT DURING EARLY PREGNANCY OF CLONED FETUSES DERIVED FROM BOVINE FETAL FIBROBLASTS AT THE EARLY G1 AND G0 PHASES

2009 ◽  
Vol 21 (1) ◽  
pp. 117
Author(s):  
A. Ideta ◽  
K. Hayama ◽  
M. Urakawa ◽  
K. Tsuchiya ◽  
Y. Nakamura ◽  
...  
Keyword(s):  
Amniotic Fluid ◽  
Early Pregnancy ◽  
Prostaglandin F2α ◽  
Fluid Volume ◽  
Efficient Production ◽  
Morphological Development ◽  
Amniotic Fluid Volume ◽  
Fetal Fibroblasts ◽  
G0 Phase ◽  
Parthenogenetic Embryos

Enhanced development of bovine somatic cell nuclear transfer (NT) embryos to full term has been achieved using fibroblasts at the early G1 (eG1) phase instead of cells at the quiescent (G0) phase (Urakawa et al. 2004 Theriogenology 62, 714–728). The high abortion rate and abnormal placental development of NT embryos using G0 phase cells is related to the low formation rate of embryonic disks and the aberrant development of the trophectoderm in utero until Day 14 of gestation (Ideta et al. 2007 Cloning Stem Cells 9, 571–580). The purpose of this study was to examine the morphological development of conceptuses such as fetuses and fetal membranes in the early pregnancy of NT embryos using eG1 phase cells (eG1-NT embryos) and G0 phase cells (G0-NT embryos). Blastocysts derived from eG1-NT and G0-NT embryos were transferred to recipient heifers, and the conceptuses at Day 50 of gestation were retrieved nonsurgically using prostaglandin F2α (PGF2α) and oxytocin (Lavoir and Betteridge 1996 J. Reprod. Fertil. 106, 95–100). In vitro-fertilized (IVF), parthenogenetic and artificially inseminated (AI) embryos were used as controls. Data were analyzed using chi-square test and Student’s t-test. Pregnancy rates at Day 30 of recipient heifers carrying eG1-NT, G0-NT, IVF, parthenogenetic, and AI embryos were similar (57 to 100%; 4/7 to 8/8). Two recipient heifers carrying parthenogenetic embryos returned to estrus between Day 30 and 50 of gestation, whereas all other pregnancies remained viable. Most fetuses at Day 50 of gestation of all experiment groups (20/24) were recovered nonsurgically by several PGF2α and oxytocin treatments. The recovery rates of normal fetuses derived from eG1-NT embryos (83%, 5/6), IVF embryos (80%, 4/5), and AI embryos (88%, 7/8) were greater than those of G0-NT embryos (33%, 2/6) and parthenogenetic embryos (0%, 0/7). The amniotic fluid volume of G0-NT embryos was significantly greater than that of AI embryos (P < 0.05). But the amniotic fluid volume of eG1-NT embryos was the same as that of AI embryos (P > 0.05). The fetal weights of eG1-NT and IVF embryos were significantly greater than the fetal weight of AI embryos (P < 0.05). Our results suggest that efficient production of cloned offspring is possible by NT using donor cells that are in the early G1 phase.

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