Pengetahuan dan Sikap Remaja Anak Jalanan tentang Dismenore Primer dan Senam Dismenore di Komunitas Peduli Anak Jalanan Medan

Primary dysmenorrhea is defined as cramping pain in the lower abdomen that occurs at the start of menstruation in the absence of identifiable pelvic disease. It is one of the most common gynecological symptoms among adolescents and adult women with a prevalence of 45%-95%. Overproduction of uterine prostaglandins is the most widely accepted explanation for the pathogenesis. Excessive release of prostaglandins, especially prostaglandin F2 alpha, which is derived from endometrial secretions from menstrual fluid, is believed to cause the condition. Primary dysmenorrhea has different degrees of negative impact on women's physical, psychological and social functioning, which can result in adolescent and adult women being out of school and absent from work. The purpose of this study was to identify the knowledge and attitudes of street children towards primary dysmenorrhea and dysmenorrhea exercise. The research design is descriptive. The sampling technique is total sampling. The number of samples is 45 people. The sample criteria for street children are teenage girls aged 10-18 years, unmarried, already menstruating, can read and write well. Data analysis is descriptive. The results of the study obtained that the majority of street children's knowledge of adolescents was good (53.3%), dysmenorrhea intensity was moderate pain with a score range of 4-7 (68.9%) and adolescent attitudes were negative (71.1%). Knowledge of respondents still needs to be improved to form a positive attitude. It is recommended to the head of KOPA to cooperate with health workers to conduct health education about primary dysmenorrhea on a regular basis to adolescent street children.

Meloxicam and Dexamethasone Administration as Anti-Inflammatory Compounds to Sows Prior to Farrowing Does Not Improve Lactation Performance

The aim of this experiment was to determine whether administration of an anti-inflammatory compound to sows prior to farrowing would, via reduced pain and inflammation, increase piglet survival and growth. At day 114 of gestation, multiparous sows were randomly allocated to one of the following treatments: Control (n = 43), which received 10 mL saline, NSAID (n = 55) which received 0.4 mg/kg meloxicam and SAID (n = 54) which received 0.1 mg/kg dexamethasone. Treatments were applied again on day 116 if farrowing had not occurred. There was no treatment effect on piglets born alive or dead from parity two to four sows but in those of parity five and older, NSAID administration reduced the number of piglets born alive and increased the number of piglets born dead (p < 0.05). Sow rectal temperature and incidence of mastitis were unaffected by treatment (p > 0.05). Lactation day two plasma concentrations of cortisol, prostaglandin F2 alpha metabolite and haptoglobin did not differ among treatments (p > 0.05). Treatment effects were not observed in liveborn piglet mortality at any age, or litter weight at day 21 (p > 0.05). Average feed intake during lactation was increased by both NSAID and SAID treatments (p = 0.001). The use of meloxicam prior to farrowing should be avoided as it reduced the number of piglets born alive and did not improve piglet survival and growth.

Evaluation of the 7 and 7 synch protocol for control of the estrous cycle among beef cows prior to fixed-time artificial insemination with sex-sorted or conventional semen

Experiment 1 was conducted to compare 7 and 7 Synch and the 7-day CO-Synch + controlled internal drug release (CIDR[copyright]) protocols prior to fixed-time artificial insemination (FTAI) of beef cows with conventional or sex-sorted semen. Cows (n = 1,538) were blocked based on age and days postpartum (DPP) and randomly assigned to protocol and semen type. Cows assigned to 7-day CO-Synch + CIDR (n = 769) received administration of gonadotropin-releasing hormone (GnRH) and insertion of an intravaginal progesterone-releasing insert (CIDR) on Day -10, and administration of prostaglandin F2[alpha] (PG) coincident with CIDR removal on Day -3. Cows assigned to 7 and 7 Synch (n = 769) received PG and insertion of CIDR on Day -17, GnRH on Day -10, and PG coincident with CIDR removal on Day -3. Estrus detection aids were applied to all cows. Cows received FTAI 66 h after CIDR removal with conventional (20 x 106 cells per unit) or sex-sorted (4 x 106 cells per unit) semen. Estrus expression was affected by protocol (P = 0.01) by protocol x DPP (P = 0.0004), with 7 and 7 Synch (82 percent; 629/769) resulting in a greater proportion of cows expressing estrus (82 percent [629/769] versus 64 percent [492/769]), especially among cows with greater DPP. Pregnancy rates to FTAI were reduced (P < 0.0001) when using sex-sorted semen but greater among cows treated with 7 and 7 Synch (conventional semen: 72 percent [280/389]; sex-sorted semen: 52 percent [199/380]) compared with 7-day CO-Synch + CIDR (conventional semen: 61 percent [233/383]; sex-sorted semen: 44 percent [171/386]). Experiment 2 was designed to evaluate later timing of fixed-time artificial insemination (FTAI) with sex-sorted semen among postpartum beef cows following the 7 and 7 Synch protocol, with the hypothesis that later timing would result in increased pregnancy rates (P/AI) among cows that expressed estrus prior to FTAI. Beef cows (n=414) were blocked based on age and days postpartum (DPP) and randomly assigned to receive FTAI at 66 or 72 h after administration of prostaglandin F2[alpha] (PG). Estrus was synchrized using the 7 and 7 Synch protocol, which consists of administration of PG (500 [mu]g cloprostenol) and insertion of an intravaginal progesterone-releasing insert (CIDR; 1.38 g progesterone) on Day 0, gonadotropin-releasing hormone (GnRH; 100 [mu]g gonadorelin) on Day 7, and PG coincident with CIDR removal on Day 14. Estrus detection aids (EstrotectTM) were applied to all cows on Day 14, and activation status was recorded at fixed-time artificial insemination (FTAI) on Day 17. All cows that expressed estrus prior to FTAI received sex-sorted semen (4 x 106 cells per unit; SexedULTRA 4MTM). The proportion of cows expressing estrus prior to FTAI did not differ between treatments at this power of test (66 h: 71 percent [146/205]; 72 h: 76 percent [158/209]). Additionally, P/AI of estrous cows inseminated with sex-sorted semen did not differ between treatments (66 h: 45 percent [68/146]; 72 h: 40 percent [63/158]). In conclusion, later timing of FTAI following the 7 and 7 Synch protocol failed to improve P/AI of estrous cows inseminated h sex-sorted semen.

The Role of Prostaglandins in Different Types of Cancer

The prostaglandins constitute a family of lipids of 20 carbon atoms that derive from polyunsaturated fatty acids such as arachidonic acid. Traditionally, prostaglandins have been linked to inflammation, female reproductive cycle, vasodilation, or bronchodilator/bronchoconstriction. Recent studies have highlighted the involvement of these lipids in cancer. In this review, existing information on the prostaglandins associated with different types of cancer and the advances related to the potential use of them in neoplasm therapies have been analyzed. We can conclude that the effect of prostaglandins depends on multiple factors, such as the target tissue, their plasma concentration, and the prostaglandin subtype, among others. Prostaglandin D2 (PGD2) seems to hinder tumor progression, while prostaglandin E2 (PGE2) and prostaglandin F2 alpha (PGF2α) seem to provide greater tumor progression and aggressiveness. However, more studies are needed to determine the role of prostaglandin I2 (PGI2) and prostaglandin J2 (PGJ2) in cancer due to the conflicting data obtained. On the other hand, the use of different NSAIDs (non-steroidal anti-inflammatory drugs), especially those selective of COX-2 (cyclooxygenase 2), could have a crucial role in the fight against different neoplasms, either as prophylaxis or as an adjuvant treatment. In addition, multiple targets, related to the action of prostaglandins on the intracellular signaling pathways that are involved in cancer, have been discovered. Thus, in depth research about the prostaglandins involved in different cancer and the different targets modulated by them, as well as their role in the tumor microenvironment and the immune response, is necessary to obtain better therapeutic tools to fight cancer.

Some Aspects of Reproductive Performance of Red Sokoto Goat Does Post Synchronization with Prostaglandin F2-Alpha And Progesterone Sponges

A study on reproduction of 52 Red Sokoto Goat (RSG) does was conducted to evaluate some aspects of their reproductive performance for clinical application and as an update. Does were randomly divided into 18, 18 and 16 as prostaglandin F2-alpha (PGF2α), progesterone sponges (P4S) and control groups respectively. Double injection protocol of PGF2α, 12-days apart, and P4S inserted for 12-days were used to synchronize oestrus, while the control group received no treatment. Thirteen bucks were used, seven as breeders and six as heat detectors. Oestrus detection employed visual observation and apronisation. Standing to be mounted was the cardinal sign of oestrus. Breeding was by hand-mating and at detected oestrus. Results indicated heterosexual and homosexual mounting, thin stringy clear mucous discharge and standing-to-be-mounted as signs of oestrus. Oestrus response rate was 100 %, 94.4 % and 75.0 % for PGF2α, P4S and Control respectively; P4S retention rate was 94%. Effect of synchronization agent on on-set of oestrus was 15.86 + 0.73 h (PGF2α), 15.08 + 0.84 h (P4S) and 17.73 + 0.85 h (Control), while parity on on-set of oestrus was 12.12 + 1.87 h (first), 17.77 + 0.77 h (second) and 18.79 + 1.95 h (third). Effect of synchronization agent on duration of oestrus was 44.76 + 2.13 h, 45.78 + 2.46 h and 42.40 + 2.50 h for PGF2Α P4S and Control respectively, while parity on duration of oestrus was 42.26 + 5.48 h (first), 45.02 + 2.27 h (second) and 45.67 + 5.73 h (third). There were no significant differences (P > 0.05) for oestrus on-set and duration. Overall pregnancy and conception rates were 65.4 % and 72.3 % respectively, kidding rate was 76.5 %, abortion rate was 23.5 % and late embryonic mortality rate was 26.5 %. Mean gestation were 146.29 + 1.59 and 146.63 + 1.64 for single and twin births respectively. Age, parity and body condition score of dam had significant effect on litter size (P < 0.05). It was concluded that some aspects of the reproductive performance of the RSG does studied following oestrus synchronization with PGF2α and P4S had clinical application, good and acceptable. Key Words: Reproductive, Red Sokoto, Does, Prostaglandin F2-alpha and Progesterone.

Prostaglandin F2 Alpha Triggers the Disruption of Cell Adhesion with Cytokeratin and Vimentin in Bovine Luteal Theca Cells

Intermediate filaments (IFs) maintain cell–cell adhesions and are involved in diverse cellular processes such as cytokinesis, cell migration and the maintenance of cell structure. In this study, we investigated the influence of prostaglandin F2 alpha (PGF2α) on cytokeratin and vimentin IFs, Rho-associated protein kinase (ROCK), and cell-cell adhesion in bovine luteal theca cells (LTCs). The luteal cells were isolated from bovine corpus luteum (CL), and the LTCs were treated with 0, 0.01, 0.1 and 1.0 mM PGF2α. Cytokeratin, vimentin and desmoplakin proteins were disrupted and the ROCK protein was significantly increased in PGF2α-treated LTCs. In addition, cell–cell adhesion was significantly (p < 0.05) decreased in the PGF2α-induced LTCs compared to control group (0 mM PGF2α). In conclusion, PGF2α affected the adhesion of cell to cell via disruption of desmoplakin, cytokeratin and vimentin, additionally increasing ROCK in bovine LTCs. These results may provide a better understanding of the mechanism of bovine CL regression.

Deciphering the functional role of EGR1 in Prostaglandin F2 alpha induced luteal regression applying CRISPR in corpus luteum of buffalo

Background PGF2α is essential for the induction of the corpus luteum regression which in turn reduces progesterone production. Early growth response (EGR) proteins are Cys2-His2-type zinc-finger transcription factor that are strongly linked to cellular proliferation, survival and apoptosis. Rapid elevation of EGR1 was observed after luteolytic dose of PGF2α. EGR1 is involved in the transactivation of many genes, including TGFβ1, which plays an important role during luteal regression. Methods The current study was conducted in buffalo luteal cells with the aim to better understand the role of EGR1 in transactivation of TGFβ1 during PGF2α induced luteal regression. Luteal cells from mid stage corpus luteum of buffalo were cultured and treated with different doses of PGF2α for different time durations. Relative expression of mRNAs encoding for enzymes within the progesterone biosynthetic pathway (3βHSD, CYP11A1 and StAR); Caspase 3; AKT were analyzed to confirm the occurrence of luteolytic event. To determine if EGR1 is involved in the PGF2α induced luteal regression via induction of TGFβ1 expression, we knocked out the EGR1 gene by using CRISPR/Cas9. Result The present experiment determined whether EGR1 protein expression in luteal cells was responsive to PGF2α treatment. Quantification of EGR1 and TGFβ1 mRNA showed significant up regulation in luteal cells of buffalo at 12 h post PGF2α induction. In order to validate the role of PGF2α on stimulating the expression of TGFβ1 by an EGR1 dependent mechanism we knocked out EGR1. The EGR1 ablated luteal cells were stimulated with PGF2α and it was observed that EGR1 KO did not modulate the PGF2α induced expression of TGFβ1. In PGF2α treated EGR1 KO luteal cell, the mRNA expression of Caspase 3 was significantly increased compared to PGF2α treated wild type luteal cells maintained for 12 h. We also studied the influence of EGR1 on steroidogenesis. The EGR1 KO luteal cells with PGF2α treatment showed no substantial difference either in the progesterone concentration or in StAR mRNA expression with PGF2α-treated wild type luteal cells. Conclusion These results suggest that EGR1 signaling is not the only factor which plays a role in the regulation of PGF2α induced TGFβ1 signaling for luteolysis.

Attainment and Maintenance of Pubertal Cyclicity May Predict Reproductive Longevity in Beef Heifers†

We hypothesized the manner that heifers achieve puberty may indicate their future reproductive longevity. Heifers with discontinued or delayed cyclicity during puberty attainment may have irregular reproductive cycles, anovulation, and infertility in their first breeding season contributing to a shorter reproductive lifespan. Therefore, plasma progesterone (P4) was measured from weaning to breeding on 611 heifers born 2012–2017 and four pubertal classifications were identified: 1) Early; P4 ≥ 1 ng/ml &lt; March 12 with continued cyclicity, 2) Typical; P4 ≥ 1 ng/ml ≥ March 12 with continued cyclicity, 3) Start-Stop; P4 ≥ 1 ng/ml but discontinued cyclicity, and 4) Non-Cycling; no P4 ≥ 1 ng/ml. Historical herd records indicated that 25% of heifers achieved puberty prior to March 12th in the 10 years prior to the study. Start-Stop and Non-Cycling yearling heifers were lighter indicating reduced growth and reproductive maturity traits compared to Early/Typical heifers. In addition, Non-Cycling/Start-Stop heifers were less responsive to prostaglandin F2 alpha (PGF2α) to initiate estrous behavior and ovulation to be artificially inseminated. Non-Cycling heifers had fewer reproductive tract score-5 and reduced numbers of calves born in the first 21-days-of-calving during their first breeding season. Within the Start-Stop classification, 50% of heifers reinitiated cyclicity with growth traits and reproductive parameters that were similar to heifers in the Early/Typical classification while those that remained non-cyclic were more similar to heifers in the Non-Cycling group. Thus, heifers with discontinued cyclicity or no cyclicity during puberty attainment had delayed reproductive maturity resulting in subfertility and potentially a shorter reproductive lifespan.

Effects of Beta-carotene Administration on Fertility in Lactating Dairy Cows

Background: Beta-(β-) carotene, is the precursor to vitamin A, in particular, has some potential benefits on reproduction. The main objective of this study was to investigate the efficacy of β-carotene administration on fertility following either prostaglandin F2 alpha (PGF) induced estrus or Ovsynch protocol in lactating dairy cows.Methods: Cows with at 47±3 postpartum days were divided into two groups: β-carotene group (βC, n=139) was treated with injectable β-carotene while untreated cows served as control (CON, n=227). In both groups, PGF was administered and heatmount detectors were applied at 54±3 days postpartum. Cows detected in estrus after PGF were inseminated. Cows that had not been detected in estrus were divided into two groups 7 days after PGF administration; βC-OVS (n=137) and CON-OVS (n=89). Ovsynch protocol was initiated 4 days after β-carotene administration.Result: The estrus detection rate was similar between the βC and CON groups (P = 0.19). Pregnancy per AI (P/AI) on d 31 was also similar between groups (P = 0.93). In the Ovsynch protocol, ovulation to the first GnRH and ovulatory follicle diameter at the time of insemination did not differ between groups. No difference was observed in P/AI at d 31 (P = 0.13). The results of this study indicated that β-carotene administration had no effect on fertility either PGF induced estrus or Ovsynch protocol in dairy cows.

Profiles of progesterone and bovine interferon-τ in repeat breeding and non-repeat breeding Aceh cows

Aim: This study aimed at determining the profiles of progesterone and bovine interferon-τ (bIFN-τ) and the correlation between the two in repeat breeding (RB) Aceh cattle and non-RB Aceh cattle. Materials and Methods: The study was performed on five RB and five non-RB Aceh cows. These cows were subjected to estrous synchronization using the prostaglandin F2 alpha hormone, which was followed by artificial insemination (AI). Serum samples were collected on days 5, 6, 7, 15, 16, and 17 after AI to measure the concentration of progesterone at the beginning and end of the luteal phase and from days 14 to 18 after AI to measure the concentration of bIFN-τ. The concentrations of progesterone and bIFN-τ were determined using enzyme-linked immunosorbent assay. Pregnancy examinations were performed by ultrasonography on days 25, 35, 45, and 55 after AI. Data for progesterone and bIFN-τ concentrations were analyzed using the Mann–Whitney and t-tests, and the correlation between progesterone and bIFN-τ was analyzed using the Spearman correlation test. Results: The average concentration of progesterone in RB Aceh cows tended to be lower than non-RB Aceh cows, but it was not significantly different (p>0.05). Similar results also found in the concentration of bIFN-τ which RB Aceh cows tended to have lower bIFN-τ concentrations compared to non-RB Aceh cows, but it was also not significantly different (p>0.05). Moreover, the concentrations of progesterone and bIFN-τ in RB and non-RB Aceh cows did not show a significant correlation (p>0.05). These results of the ultrasonography showed that non-RB Aceh cows were pregnant from day 25 to day 55 after AI, whereas RB Aceh cows were not pregnant and had early embryonic death. Conclusion: The concentrations of progesterone and bIFN-τ in non-RB Aceh cows tended to be higher than those in RB Aceh cows, although, it was not significantly different. Non-RB Aceh cows were able to maintain pregnancy until day 55, whereas RB Aceh cows were diagnosed with early embryonic death before day 25 after AI.