Coimmobilized enzyme columns in determining serum creatinine using creatininase, creatinase and sarcosine oxidase by flow-injection analysis and chemiluminescence detection

1992 ◽  
Vol 262 (2) ◽  
pp. 315-321 ◽  
Author(s):  
Masayoshi Tabata ◽  
Masayuki Totani ◽  
Jiro Endo
1979 ◽  
Vol 25 (9) ◽  
pp. 1635-1638 ◽  
Author(s):  
G Rule ◽  
W R Seitz

Abstract In "flow-injection analysis," the sample is inserted into a stream of reagent by use of a sample-injection valve. Mixing occurs downstream from the valve in a coil of tubing. With chemiluminescence detection this coil is positioned in front of a photomultiplier. We have evaluated the system for detection of hydrogen peroxide, using the luminol reaction with cupric ion as a catalyst. The effects of flow rate, sample volume, and reaction kinetics on the magnitude, duration, and repeatability of the chemiluminescent response have been evaluated. Precisions of 1 to 2% relative standard deviation on replicate measurements are readily achievable. A sample throughput of six samples per minute is possible with very little peak overlap. This detection system can be coupled to any process in which peroxide is generated.


1993 ◽  
Vol 65 (23) ◽  
pp. 3489-3492 ◽  
Author(s):  
Xincheng. Hu ◽  
Norimichi. Takenaka ◽  
Shiro. Takasuna ◽  
Masaru. Kitano ◽  
Hiroshi. Bandow ◽  
...  

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