Nitrocellulose filter binding: Quantitation of the histidyl-tRNA-ATP phosphoribosyltransferase complex

1979 ◽  
Vol 92 (1) ◽  
pp. 22-30 ◽  
Author(s):  
Jimmy D. Allen ◽  
Stanley M. Parsons
1988 ◽  
Vol 34 (7) ◽  
pp. 1523-1523 ◽  
Author(s):  
Y Endo ◽  
T Iwata ◽  
H Nagasaki ◽  
K Miyai

Gene ◽  
1981 ◽  
Vol 13 (1) ◽  
pp. 75-87 ◽  
Author(s):  
Harlee S. Strauss ◽  
Rebecca S. Boston ◽  
M.Thomas Record ◽  
Richard R. Burgess

2013 ◽  
Vol 60 (2) ◽  
pp. 29-36
Author(s):  
Mikušová V. ◽  
Lukačovičová O. ◽  
Havránek E.

Abstract The contents of selected elements (Mn, Fe, Co, Ni, Cu, Zn, Hg and Pb) in samples of loose tea of agrimony “Repikovy čaj” (Agrimonia eupatoria L.) were studied in this work. Identification and determination of the selected elements content were performed using X-ray fluorescence spectrometry - the nuclear analytical method based on the interaction between low-energy gamma and X radiation and analysed substance. Solid form samples were pressed in tablets and directly analysed. Prepared infusions were analysed after the preconcentration of the elements using precipitation with thioacetamide and ammonium diethyldithiocarbamate with subsequent filtration of created precipitates through a nitrocellulose filter. Detection limits obtained from both methods were compared and their suitability for elemental analysis of plant preparations was discussed.


1979 ◽  
Vol 178 (1) ◽  
pp. 233-236 ◽  
Author(s):  
S Sperti ◽  
L Montanaro

Ricin and modeccin do not affect the total number of ribosomes to which phenylalanyl-tRNA becomes bound in the EF 1-dependent reaction. Previous inconsistencies resulted from the use of the nitrocellulose-filter technique, which overestimates the number of control ribosomes engaged in the binding reaction if trace amounts of EF 2 contaminate the ribosomal preparations.


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